The legacy of Biosphere 2 for the study of biospherics and closed ecological systems.

The unprecedented challenges of creating Biosphere 2, the world's first laboratory for biospherics, the study of global ecology and long-term closed ecological system dynamics, led to breakthrough developments in many fields, and a deeper understanding of the opportunities and difficulties of material closure. This paper will review accomplishments and challenges, citing some of the key research findings and publications that have resulted from the experiments in Biosphere 2. Engineering accomplishments included development of a technique for variable volume to deal with pressure differences between the facility and outside environment, developing methods of atmospheric leak detection and sealing, while achieving new standards of closure, with an annual atmospheric leakrate of less than 10%, or less than 300 ppm per day. This degree of closure permitted detailed tracking of carbon dioxide, oxygen, and trace gases such as nitrous oxide and ethylene over the seasonal variability of two years. Full closure also necessitated developing new approaches and technologies for complete air, water, and wastewater recycle and reuse within the facility. The development of a soil-based highly productive agricultural system was a first in closed ecological systems, and much was learned about managing a wide variety of crops using non-chemical means of pest and disease control. Closed ecological systems have different temporal biogeochemical cycling and ranges of atmospheric components because of their smaller reservoirs of air, water and soil, and higher concentration of biomass, and Biosphere 2 provided detailed examination and modeling of these accelerated cycles over a period of closure which measured in years. Medical research inside Biosphere 2 included the effects on humans of lowered oxygen: the discovery that human productivity can be maintained with good health with lowered atmospheric oxygen levels could lead to major economies on the design of space stations and planetary/lunar settlements. The improved health resulting from the calorie-restricted but nutrient dense Biosphere 2 diet was the first such scientifically controlled experiment with humans. The success of Biosphere 2 in creating a diversity of terrestrial and marine environments, from rainforest to coral reef, allowed detailed studies with comprehensive measurements such that the dynamics of these complex biomic systems are now better understood. The coral reef ecosystem, the largest artificial reef ever built, catalyzed methods of study now being applied to planetary coral reef systems. Restoration ecology advanced through the creation and study of the dynamics of adaptation and self-organization of the biomes in Biosphere 2. The international interest that Biosphere 2 generated has given new impetus to the public recognition of the sciences of biospheres (biospherics), biomes and closed ecological life systems. The facility, although no longer a materially-closed ecological system, is being used as an educational facility by Columbia University as an introduction to the study of the biosphere and complex system ecology and for carbon dioxide impacts utilizing the complex ecosystems created in Biosphere '.The many lessons learned from Biosphere 2 are being used by its key team of creators in their design and operation of a laboratory-sized closed ecological system, the Laboratory Biosphere, in operation as of March 2002, and for the design of a Mars on Earth(TM) prototype life support system for manned missions to Mars and Mars surface habitats. Biosphere 2 is an important foundation for future advances in biospherics and closed ecological system research.     

“Dakota Sun”-the next generation solar powered racingvehicle

Based on the experience gained from Sunrayce '95, the Solar Motion Team has made many changes to the design of the next generation solar car. These changes have resulted in a vehicle that is very different from the “Solar Rolar”, The Dakota Sun is a three wheeled vehicle with separate cab and solar array. This design allows for improved aerodynamics, decreased weight, lower rolling resistance, and ease of manufacture compared to the four wheeled catamaran used in the last race. However, this design sacrifices total enclosed wheel base area, additional room for components, and added power from side solar panels, The major objectives for the team's redesigned Sunrayce '97 entry are: systems integration; decrease the weight of the car; decrease aerodynamic drag; more efficient use of available energy; and increased driver safety. The team has set a standard to use the latest available technology. Although this increases the complexity of the components, by using a systems engineering approach the “Dakota Sun” has evolved into a more integrated vehicle. This philosophy of integrated design has resulted in great improvements in mechanical design and manufacturing techniques, as well as electrical innovations. The major design changes evident from the original Sunraycen '95 vehicle are the result of an evolutionary design process that has produced the highly competitive Sunraycel '97 design outlined in this article     

Anthropogenic and natural causes of twentieth century temperature change

We analyse spatio-temporal patterns of near-surface temperature change to provide an attribution of twentieth century climate change. We apply an ``optimal detection' methodology to seasonal and annual data averaged over a range of spatial and temporal scales. We find that solar effects may have contributed significantly to the warming in the first half of the century although this result is dependent on the reconstruction of total solar irradiance that is used. In the latter half of the century, we find that anthropogenic increases in greenhouses gases are largely responsible for the observed warming, balanced by some cooling due to anthropogenic sulphate aerosols, with no evidence for significant solar effects.     

Children's control of reference systems in spatial tasks: Foundations of spatial cognitive skill?

Two experiments are presented, one that focused on the abilities of 3- through 8-year-old children to use frames of reference as means for solving a search task in a small-scale perceived space and one that focused on the abilities of 8- through 11-year-old children to use different frames of reference to answer questions about the locations of objects in an environment they had read about. Experiment 1 revealed developmental improvement in the consistent application of object-, place-, and viewer-based frames of reference, with scale of space impacting the pattern of age-related improvement. Experiment 2 showed that young readers' first successes with switching from one reference system to another in representational space involved a vantage point perspective, a viewer-based ``gaze tour' of a small-scale area. Although disparate in focus and method, the studies point to the need for a new theoretical framework for examining developmental issues in the application of spatial frames of reference.     

Supraglacial sulfur springs and associated biological activity in the Canadian high arctic-signs of life beneath the ice

Unique springs, discharging from the surface of an arctic glacier, release H(2)S and deposit native sulfur, gypsum, and calcite. The presence of sulfur in three oxidation states indicates a complex series of redox reactions. Physical and chemical conditions of the spring water and surrounding environment, as well as mineralogical and isotopic signatures, suggest biologically mediated reactions. Cell counts and DNA analyses confirm bacteria are present in the spring system, and a limited number of sequenced isolates suggests that complex communities of bacteria live within the glacial system.     

Starting to partner with NASA in space and Earth science

NASA research programs offer many opportunities for productive partnerships with investigators in other countries. While spacecraft projects are complex and very expensive, there are other, lower-cost partnerships that can yield important scientific results and offer excellent opportunities for building up new space and Earth science programs and for training new researchers.     

The space exploration initiative Perspective from the US space science community

As NASA struggles to fund and execute its ambitious Space Station Freedom and Mission to Planet Earth programmes, President Bush has pledge the nation to a programme to return humans to the Moon to stay and to explore Mars. While not predicted on scientific grounds, this Space Exploration Initiative welcomes the support and participation of the scientific community. Success in establishing this relationship will depend on how the initiative is structured, sold and managed within the context of scientists' past experience with large, manned flight programmes.     

Synthesis of Computationally Efficient Sequential Linear Estimators

The Kalman sequential linear estimation theory, although not always utilized because the number of computations required for many systems of practical importance becomes prohibitive, allows straight-forward synthesis of optimal estimators for many complex systems. Some systems designers have chosen to ignore variables and by such a reduction in system dimension have been able to economize with regard to the number of computations. The purpose of this paper is to demonstrate a method which allows economy of computation by partitioning the system state vector; the variables to be eliminated are placed in one subsystem and the remaining variables in one or more additional subsystems. The resultant system is computationally more efficient if some variables are eliminated. This is so because the remaining states have been partitioned into two or more subsystems. The number of computations for a subsystem varies approximately as the cube of the dimension of its state vector. By operating on several subsystems of lesser dimension than that of the unpartitioned system, the number of computations is decreased; performance will deteriorate. The method for determining the partitioning tends to keep this deterioration under control; it is illustrated by application to a marine-type inertial navigation system.     

A bacterial enrichment study and overview of the extractable lipids from paleosols in the Dry Valleys, Antarctica: implications for future Mars reconnaissance

The Dry Valleys of Antarctica are one of the coldest and driest environments on Earth with paleosols in selected areas that date to the emplacement of tills by warm-based ice during the Early Miocene. Cited as an analogue to the martian surface, the ability of the Antarctic environment to support microbial life-forms is a matter of special interest, particularly with the upcoming NASA/ESA 2018 ExoMars mission. Lipid biomarkers were extracted and analyzed by gas chromatography--mass spectrometry to assess sources of organic carbon and evaluate the contribution of microbial species to the organic matter of the paleosols. Paleosol samples from the ice-free Dry Valleys were also subsampled and cultivated in a growth medium from which DNA was extracted with the explicit purpose of the positive identification of bacteria. Several species of bacteria were grown in solution and the genus identified. A similar match of the data to sequenced DNA showed that Alphaproteobacteria, Gammaproteobacteria, Bacteriodetes, and Actinobacteridae species were cultivated. The results confirm the presence of bacteria within some paleosols, but no assumptions have been made with regard to in situ activity at present. These results underscore the need not only to further investigate Dry Valley cryosols but also to develop reconnaissance strategies to determine whether such likely Earth-like environments on the Red Planet also contain life.     

Effects of simulated space radiation on immunoassay components for life-detection experiments in planetary exploration missions

Abstract The Life Marker Chip (LMC) instrument is part of the proposed payload on the ESA ExoMars rover that is scheduled for launch in 2018. The LMC will use antibody-based assays to detect molecular signatures of life in samples obtained from the shallow subsurface of Mars. For the LMC antibodies, the ability to resist inactivation due to space particle radiation (both in transit and on the surface of Mars) will therefore be a prerequisite. The proton and neutron components of the mission radiation environment are those that are expected to have the dominant effect on the operation of the LMC. Modeling of the radiation environment for a mission to Mars led to the calculation of nominal mission fluences for proton and neutron radiation. Various combinations and multiples of these values were used to demonstrate the effects of radiation on antibody activity, primarily at the radiation levels envisaged for the ExoMars mission as well as at much higher levels. Five antibodies were freeze-dried in a variety of protective molecular matrices and were exposed to various radiation conditions generated at a cyclotron facility. After exposure, the antibodies' ability to bind to their respective antigens was assessed and found to be unaffected by ExoMars mission level radiation doses. These experiments indicated that the expected radiation environment of a Mars mission does not pose a significant risk to antibodies packaged in the form anticipated for the LMC instrument. Key Words: Life-detection instruments-Planetary habitability and biosignatures-Radiation-Mars-Life in extreme environments. Astrobiology 12, 718-729.