Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

The Lightning And Radio Emission Detector (LRD) instrument

The Lightning and Radio Emission Detector (LRD) instrument will be carried by the Galileo Probe into Jupiter's atmosphere. The LRD will verify the existence of lightning in the atmosphere and will determine the details of many of its basic characteristics. The instrument, operated in its magnetospheric mode at distances of about 5, 4, 3, and 2 planetary radii from Jupiter's center, will also measure the radio frequency (RF) noise spectrum in Jupiter's magnetosphere. The LRD instrument is composed of a ferritecore radio frequency antenna ( 100 Hz to 100 kHz) and two photodiodes mounted behind individual fisheye lenses. The output of the RF antenna is analyzed both separately and in coincidence with the optical signals from the photodiodes. The RF antenna provides data both in the frequency domain (with three narrow-band channels, primarily for deducing the physical properties of distant lightning) and in the time domain with a priority scheme (primarily for determining from individual RF waveforms the physical properties of closeby-lightning).     

Development of plant protoplasts during the IML-1 mission.

During the 8 day IML-1 mission, regeneration of cell walls and cell divisions in rapeseed protoplasts were studied using the Biorack microscope onboard the Space Shuttle "Discovery". Samples from microgravity and 1g protoplast cultures were loaded on microscope slides. Visual microscopic observations were reported by the payload specialist Roberta Bondar, by down-link video transmission and by use of a microscope camera. Protoplasts grown under microgravity conditions do regenerate cell walls but to a lesser extent than under 1g. Cell divisions are delayed under microgravity. Few cell aggregates with maximum 4-6 cells per aggregate are formed under microgravity conditions, indicating that microgravity may have a profound influence on plant cell differentiation.     

Nonlinear problems of physics of the geomagnetic pulsations

The current state of research involving manifestations of nonlinearity in geomagnetic pulsations is reviewed. Traditionally, the attention of researchers was focused on the effects of resonant interaction of geomagnetic pulsations with small groups of energetic particles, which actually means the study of the quasi-linear relaxation of radiation belt ions, the modulation of auroral electron fluxes, etc. The present review concentrates on the problem of the nonlinear effect influence of pulsations on the backgroud (cold) plasma and on the geomagnetic field. This kind of interaction results in a significant modification of the plasma distribution in the magnetosphere. Self-consistent wave structures—solitons and vortices may occur as well. Such nonlinear effects contribute to physics of geomagnetic pulsations and are also of fundamental importance for general physics. Another set of more narrow problems considered in the review, is related to phenomenological modeling of fluctuational and critical phenomena in the magnetosphere. The essence of our approach is to present the magnetosphere as a black box, whose properties should be determined by the statistical characteristics of its output signals. This approach to phenomenology can be a useful supplement to the methods of microscopic modeling aimed at detecting nonlinear manifestations of geomagnetic pulsations.     

The complex Hα profile of the hypergiant HR 8752 during 1970–1992

The profiles of H observed during the 1970–1992 period in the binary hypergiant HR 8752 (G0 Ia) are presented. We distinguish five typical H profiles designated as A, B, C, D and E types according to the number of emission and absorption features. The profiles of H are complex and contain several emission and absorption components, with: –130 km/s in emission or absorption, –84 km/s in absorption, –49 km/s in emission and about +6 km/s in emission. All of them are rather stable in radial velocities except of the main absorption component in the P Cygni profile with –84 km/s. The frequency of appearance and the periods of duration of the occurrence of the components is discussed. The duration times range between about 3 to 10 months for various components. The red emission component E₂ is particularly interesting. Possible explanations of its origin are discussed.A long-term acceleration of the absorption component in the P Cygni profile is found; it can be interpreted as monotonous acceleration of the stellar wind.     

The Genesis Solar-Wind Collector Materials

Genesis (NASA Discovery Mission #5) is a sample return mission. Collectors comprised of ultra-high purity materials will be exposed to the solar wind and then returned to Earth for laboratory analysis. There is a suite of fifteen types of ultra-pure materials distributed among several locations. Most of the materials are mounted on deployable panels (‘collector arrays’), with some as targets in the focal spot of an electrostatic mirror (the ‘concentrator’). Other materials are strategically placed on the spacecraft as additional targets of opportunity to maximize the area for solar-wind collection. Most of the collection area consists of hexagonal collectors in the arrays; approximately half are silicon, the rest are for solar-wind components not retained and/or not easily measured in silicon. There are a variety of materials both in collector arrays and elsewhere targeted for the analyses of specific solar-wind components. Engineering and science factors drove the selection process. Engineering required testing of physical properties such as the ability to withstand shaking on launch and thermal cycling during deployment. Science constraints included bulk purity, surface and interface cleanliness, retentiveness with respect to individual solar-wind components, and availability. A detailed report of material parameters planned as a resource for choosing materials for study will be published on a Genesis website, and will be updated as additional information is obtained. Some material is already linked to the Genesis plasma data website (genesis.lanl.gov). Genesis should provide a reservoir of materials for allocation to the scientific community throughout the 21st Century. This revised version was published online in August 2006 with corrections to the Cover Date.     

Transient Modeling of Synchronous Machine-Transformer-SCR Bridge

A rigorous mathematical model of a synchronouschine-machine-transformer-thyristor bridge unit is presented in state-space form with coefficients that are explicit functions of standard parameters. The developed model has a minimum order for each of the various operation modes of the bridge, and its form is such that it can be readily interfaced with any type of dc network connected to the bridge. In addition, the model has the capability to consider different types of transformer winding connections. In a digital simulation, the explicit form of the state model eliminates the need of matrix inversions at each integration step. This and the use of the ?, ? reference frame result in a high computing efficiency and accuracy. As an illustration, simulation results are shown of a case study where the unit is part of a high-power pulse generating system.     

Variable Speed Wind Energy Conversion Using Synchronous Machine

A power generation scheme using a synchronous generator with an excitation control system driven by a variable-speed wind turbine is described. The proposed scheme has been analyzed over a wide speed range and under wind gusting conditions. Performance characteristics show that it is feasible to use variable-speed fixed-pitch wind turbines to generate electric power.     

Observational Manifestation of Chaos in the Gaseous Disk of the Grand Design Spiral Galaxy NGC 3631

The main goal of the paper is to demonstrate the presence of chaotic trajectories in the gaseous disk of a real spiral galaxy. As an example we have chosen NGC 3631. First, we show the stationarity of the 3-D velocity field restored from the observed line-of-sight velocity field of the gaseous disk. That allows to analyse behaviour of the trajectories of the fluid particles (gas clouds) in the disk, calculating the corresponding observed streamlines. We estimate the Lyapunov characteristic numbers using their independence of the metrics and show the existence of chaotic trajectories outside the vortices which are present in the velocity field, and in the vicinity of the saddle point. Related spectra of the stretching numbers for some trajectories are also calculated.     

Augmenting the Linear Variance Equation

A technique is developed to convert the covariance analysis ofcertain time-varying linear systems into time-invariant form, thusallowing an arbitrary time step for the solution of the differentialequations. The result is accomplished by augmenting the linearvariance equation and augmenting the state vector if necessary. Themethod is illustrated with an example showing the covariancepropagation of ring laser gyro errors in a strapdown inertial systeminto navigation errors during turns.