Lichens survive in space: results from the 2005 LICHENS experiment

This experiment was aimed at establishing, for the first time, the survival capability of lichens exposed to space conditions. In particular, the damaging effect of various wavelengths of extraterrestrial solar UV radiation was studied. The lichens used were the bipolar species Rhizocarpon geographicum and Xanthoria elegans, which were collected above 2000 m in the mountains of central Spain and as endolithic communities inhabiting granites in the Antarctic Dry Valleys. Lichens were exposed to space in the BIOPAN-5 facility of the European Space Agency; BIOPAN-5 is located on the outer shell of the Earth-orbiting FOTON-M2 Russian satellite. The lichen samples were launched from Baikonur by a Soyuz rocket on May 31, 2005, and were returned to Earth after 16 days in space, at which time they were tested for survival. Chlorophyll fluorescence was used for the measurement of photosynthetic parameters. Scanning electron microscopy in back-scattered mode, low temperature scanning electron microscopy, and transmission electron microscopy were used to study the organization and composition of both symbionts. Confocal laser scanning microscopy, in combination with the use of specific fluorescent probes, allowed for the assessment of the physiological state of the cells. All exposed lichens, regardless of the optical filters used, showed nearly the same photosynthetic activity after the flight as measured before the flight. Likewise, the multimicroscopy approach revealed no detectable ultrastructural changes in most of the algal and fungal cells of the lichen thalli, though a greater proportion of cells in the flight samples had compromised membranes, as revealed by the LIVE/DEAD BacLight Bacterial Viability Kit. These findings indicate that most lichenized fungal and algal cells can survive in space after full exposure to massive UV and cosmic radiation, conditions proven to be lethal to bacteria and other microorganisms. The lichen upper cortex seems to provide adequate protection against solar radiation. Moreover, after extreme dehydration induced by high vacuum, the lichens proved to be able to recover, in full, their metabolic activity within 24 hours.     

An examination of the carbon isotope effects associated with amino acid biosynthesis

Stable carbon isotope ratios (delta(13)C) were determined for alanine, proline, phenylalanine, valine, leucine, isoleucine, aspartate (aspartic acid and asparagine), glutamate (glutamic acid and glutamine), lysine, serine, glycine, and threonine from metabolically diverse microorganisms. The microorganisms examined included fermenting bacteria, organotrophic, chemolithotrophic, phototrophic, methylotrophic, methanogenic, acetogenic, acetotrophic, and naturally occurring cryptoendolithic communities from the Dry Valleys of Antarctica. Here we demonstrated that reactions involved in amino acid biosynthesis can be used to distinguish amino acids formed by life from those formed by nonbiological processes. The unique patterns of delta(13)C imprinted by life on amino acids produced a biological bias. We also showed that, by applying discriminant function analysis to the delta(13)C value of a pool of amino acids formed by biological activity, it was possible to identify key aspects of intermediary carbon metabolism in the microbial world. In fact, microorganisms examined in this study could be placed within one of three metabolic groups: (1) heterotrophs that grow by oxidizing compounds containing three or more carbon-to-carbon bonds (fermenters and organotrophs), (2) autotrophs that grow by taking up carbon dioxide (chemolitotrophs and phototrophs), and (3) acetoclastic microbes that grow by assimilation of formaldehyde or acetate (methylotrophs, methanogens, acetogens, and acetotrophs). Furthermore, we demonstrated that cryptoendolithic communities from Antarctica grouped most closely with the autotrophs, which indicates that the dominant metabolic pathways in these communities are likely those utilized for CO(2 )fixation. We propose that this technique can be used to determine the dominant metabolic types in a community and reveal the overall flow of carbon in a complex ecosystem.     

Superlow-Frequency MHD-Eigenoscillations With a Discrete Spectrum in Parabolic Model of Magnetosphere

A new concept of the global magnetospheric resonator is suggested for fast magnetosonic waves in which the role of the resonator is played by the near part of the plasma sheet. It is shown that the magnetosonic wave is confined in this region of the magnetosphere throughout its boundaries. The representative value of the resonator's eigenfrequency estimated at f ≈ 1 mHz is in good agreement with observational data on ultraThe theory explains the ground-based localization of the oscillations observed in the midnight-morning sector of the high-latitude magnetosphere.      

A Mechanism of Solar Variability Effect on Radiative Balance of the Earth Atmosphere

Possible mechanisms of solar-climatic connections, which may be of importance as over short and long time intervals, are discussed. The variations of energetic balance of Earth's climatic system for the last fifty years are estimated. It is ascertained that the disbalance between the flux of solar energy that comes to the Earth and radiates to space is of 0.1% for the last ten years. The suggested mechanism makes it possible to explain not only the observed variation of the enthalpy of the Earth's climatic system for the period 1910-1980, but also the climate anomalies during last thousand years: the climate optimum in 12 century, and"small glacial period" in 16-17 centuries.      

巴尔迪克-径向铆接--满足最精确的铆接成形要求

巴尔迪克的铆接法可铆接以前被认为不可铆接的材料,并能得到完美的铆接面。材料沿径向滚动可防止产生扭应力,从而得到高质量的铆接面并具有很高的抗疲劳强度     

一种利用功率反演和线性约束最小方差算法的自适应天线

介绍了一种新的基于功率反演和线性约束最小方差的算法,以高度抑制GPS接收机的干扰信号。这种结构通过提调整天线阵列的权值,实时地接收并改变来自各方向的GPS信号,同时对不同方向的干扰信号有高的抑制比。对固定和移动的干扰都做了仿真,仿真表明这种结构有很深的零点,对固定干扰信号的抑制比可达到115dB,对移动干扰信号的抑制比可达到94dB。     

A numerical optimization of high altitude testing facility for wind tunnel experiments

High altitude test facilities are required to test the high area ratio nozzles operating at the upper stages of rocket in the nozzle full flow conditions.It is typically achieved by creating the ambient pressure equal or less than the nozzle exit pressure.On average,air/GN2is used as active gas for ejector system that is stored in the high pressure cylinders.The wind tunnel facilities are used for conducting aerodynamic simulation experiments at/under various flow velocities and operating conditions.However,constructing both of these facilities require more laboratory space and expensive instruments.Because of this demerit,a novel scheme is implemented for conducting wind tunnel experiments by using the existing infrastructure available in the high altitude testing(HAT)facility.This article presents the details about the methods implemented for suitably modifying the sub-scale HAT facility to conduct wind tunnel experiments.Hence,the design of nozzle for required area ratio A/A*,realization of test section and the optimized configuration are focused in the present analysis.Specific insights into various rocket models including high thrust cryogenic engines and their holding mechanisms to conduct wind tunnel experiments in the HAT facility are analyzed.A detailed CFD analysis is done to propose this conversion without affecting the existing functional requirements of the HAT facility.     

SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.