The effects of simulated microgravity on cultured chicken embryonic chondrocytes.

Using the cultured chicken embryonic chondrocytes as a model, the effects of simulated microgravity on the microtubular system of the cellular skeleton, extracellular matrix, alkaline phosphatase activity, intracellular free calcium concentration and mitochondrial ATP synthase activity with its oligomycin inhibition rate were studied with a clinostat. The microtubular content was measured by a flow cytometer. The decrease of microtubular content showed the impairment of the cellular skeleton system. Observation on the extracellular matrix by the scanning electron microscopy showed that it decreased significantly after rotating, and the fibers in the extracellular matrix were more tiny and disorderly than that of the control group. It can be concluded that the simulated microgravity can affect the secreting and assembly of the extracellular matrix. In contrast to the control, there was a time course decrease in alkaline phosphatase activity of chondrocytes, a marker of matrix mineralization. Meanwhile a significant drop in the intracellular calcium concentration happened at the beginning of rotation. These results indicate that simulated microgravity can suppress matrix calcification of cultured chondrocytes, and intracellular free calcium may be involved in the regulation of matrix calcification as the second signal transmitter. No significant changes happened in the mitochondrial ATP synthase activity and its oligomycin inhibition rate. Perhaps the energy metabolism wasn't affected by the simulated microgravity. The possible mechanisms about them were discussed.     

Demonstration of a zinc bromine battery in an electric vehicle

The zinc bromine battery is a high energy density battery that utilizes low cost materials. The battery is of unique construction utilizing plastic storage tanks for the zinc bromide electrolyte and plastic bipolar electrode stacks. This paper briefly describes the zinc bromine battery technology and the experience gained in installing and operating an electric vehicle with this advanced system. The described electric vehicle (The “T-Star”) was tested in March 1993 on the Chrysler Proving Grounds in Phoenix, Arizona and it participated in the May 1993 American Tour de Sol capturing second place over all and first place in the student division     

Galileo Net Flux Radiometer experiment

The Galileo Net Flux Radiometer (NFR) is a Probe instrument designed to measure the vertical profile of upward and net radiation fluxes in five spectral bands spanning the range from solar to far infrared wavelengths. These unique measurements within Jupiter's atmosphere, from which radiative heating and cooling profiles will be derived, will contribute to our understanding of Jovian atmospheric dynamics, to the detection of cloud layers and determination of their opacities, and to the estimation of water vapor abundance. The NFR uses an array of pyroelectric detectors and individual bandpass filters in a sealed detector package. The detector package and optics rotate as a unit to provide chopping between views of upward and downward radiation fluxes. This arrangement makes possible the measurement of small net fluxes in the presence of large ambient fluxes. A microprocessor-controlled electronics package handles instrument operation.     

Pade approximations of probability density functions

The analysis of radar detection systems often requires extensive knowledge of the special functions of applied mathematics, and their computation. Yet, the moments of the detection random variable are often easily obtained. We demonstrate here how to employ a limited number of exactly specified moments to approximate the probability density and distribution functions of various random variables. The approach is to use the technique of Pade approximations (PA) which creates a pole-zero model of the moment generating function (mgf). This mgf is inverted using residues to obtain the densities     

Cometary origin of carbon and water on the terrestrial planets.

An early high-temperature phase of the protosolar accretion disk is implied by at least three different telltales in chondrites and confirmed by peculiarities in the dust grains of comet Halley. The existence this high-temperature phase implies a large accretion rate hence a massive early disk. This clarifies the origin of the Kuiper Belt and of the Oort cloud, those two cometary populations of different symmetry that subsist today. Later, when the dust sedimented and was removed from the thermal equilibrium with the gas phase, a somewhat lower temperature of the disk explains the future planets' densities as well as the location beyond 2.6 AU of the carbonaceous chondrite chemistry. This lower temperature remains however large enough to require an exogenous origin for all carbon and all water now present in the Earth. The later orbital diffusion of planetesimals, which is required by protoplanelary growth, is needed to explain the origin of the terrestrial biosphere (atmosphere, oceans, carbonates and organic compounds) by a veneer mostly made of comets.     

A biophysical model for estimating the frequency of radiation-induced mutations resulting from chromosomal translocations.

Gene mutations can be induced by radiation as a result of chromosomal translocations. A biophysical model is developed to estimate the frequency of this type of mutation induced by low-LET radiation. Mutations resulting from translocations are assumed to be formed by misrejoining of two DNA double strand breaks (DSB), one within the gene and one on a different chromosome. The chromosome containing the gene is assumed to occupy a spherical territory and does not overlap spatially with other chromosomes. Misrejoining between two DSB can occur only if the two DSB are closer than an interaction distance at the time of their induction. Applying the model to mutations of the hprt gene induced in G0 human lymphocyte cells by low-LET radiation, it is calculated that mutations resulting from translocations account for about 14% of the total mutations. The value of the interaction distance is determined to be 0.6 micrometers by comparing with the observed frequency of translocations in the X-chromosome.     

Fallacies in computational testing of matrix positivedefiniteness/semidefiniteness

The status of computational tests for establishing matrix positive semidefiniteness and positive definiteness is reviewed. Two pervasive real-time tests that have been used for many years in varied applications to ensure that computed covariances encountered in Kalman filter applications are positive definite and discussed. Structural representations of covariance matrices are reviewed as a prelude to constructing a counterexample and demonstrating that it refutes these real-time tests. It is maintained that the latter are bogus approaches despite the fact that they are pervasive. It is suggested that such bogus tests arose as an attempt to fill the need for a quick check (over the entire mission time) of the massive number of matrices computationally encountered in real-time applications     

Accelerating hypersonic airplanes with ground-power

The possible benefits of using ground power to accelerate the airplane on a launch ramp are quantified. A passenger-carrying hypersonic airplane leaving its loading ramp carries fuel for acceleration, cruise and landing. For example, a 230-passenger plane starting a Mach 5 trans-Pacific flight will be loaded with 80 metric tons of liquid hydrogen, up to 40% of which is burned in generating kinetic energy. Catapulting this airplane to cruise speed with ground power would reduce weight and liquid-hydrogen volume. However, there are limits. A ramp for launching a 150-metric-ton vehicle to Mach 5 with 4 g acceleration would be 38 km long and require up to 10 GW of power. In the final second, 2.8 MWh of energy is consumed along 1720 m of ramp. Possible scenarios and acceleration mechanisms are evaluated     

Magnetic resonance image enhancement using V-filter

A method for enhancing the boundaries of magnetic resonance images (MRIs) using a V-filter (a spatial nonlinear filter) is presented. By iterated V-filtering the signal-to-noise ratio of the MRI can be increased while preserving edge sharpness. A simulation experiment was carried out to evaluate the filter, using clinical brain images. The boundaries of the brain tumor and edematous region were precisely extracted by the region segmentation techniques     

Issues in deep space radiation protection.

The exposures in deep space are largely from the Galactic Cosmic Rays (GCR) for which there is as yet little biological experience. Mounting evidence indicates that conventional linear energy transfer (LET) defined protection quantities (quality factors) may not be appropriate for GCR ions. The available biological data indicates that aluminum alloy structures may generate inherently unhealthy internal spacecraft environments in the thickness range for space applications. Methods for optimization of spacecraft shielding and the associated role of materials selection are discussed. One material which may prove to be an important radiation protection material is hydrogenated carbon nanofibers.