Identification of specific gravity sensitive signal transduction pathways in human A431 carcinoma cells.

Epidermal growth factor (EGF) activates a well characterized signal transduction cascade in human A431 epidermoid carcinoma cells. The influence of gravity on EGF-induced EGF-receptor clustering and early gene expression as well as on actin polymerization and actin organization have been investigated. Different signalling pathways induced by the agents TPA, forskolin and A23187 that activate gene expression were tested for sensitivity to gravity. EGF-induced c-fos and c-jun expression were decreased in microgravity. However, constitutive beta-2 microglobulin expression remained unaltered. Under simulated weightlessness conditions EGF- and TPA-induced c-fos expression was decreased, while forskolin- and A23187-induced c-fos expression was independent of the gravity conditions. These results suggest that gravity affects specific signalling pathways. Preliminary results indicate the EGF-induced EGF-receptor clustering remained unaltered irrespective of the gravity conditions. Furthermore, the relative filamentous actin content of steady state A431 cells was enhanced under microgravity conditions and actin filament organization was altered. Under simulated weightlessness actin filament organization in steady state cells as well as in EGF-treated cells was altered as compared to the 1 G reference experiment. Interestingly the microtubule and keratin organization in untreated cells showed no difference with the normal gravity samples. This indicates that gravity may affect specific components of the signal transduction circuitry.     

Three Mile Island? Lessons for America

The great fear of a nuclear castastrophe is the prime lesson learned from the Three Mile Island accident. This fear is real and creates a major risk to our society. Although the probability of a major release of radioactivity occurring at a given plant in a given year is low, the rapidly increasin number of plants around the world increases the probability that such a catastrophe will occur someplace in the next few years. When a major release does occur, people will demand an end to the use of energy from nuclear power. We are at grave risk in not having an alternative, in not providing a choice in time. The alternatives are clearly defined. But we need to see that the resources are made available to develop them.     

High Power Needs for Commercial Satellites

This overview paper presents estimates of the photovoltaic power systems needed on commercial communications spacecraft in the year 2000. These are developed in the form of power requirements based on extrapolation of the historical growth in communications traffic and are about 5 to 15 kW. The paper also addresses the key technology drivers in these photovoltaic systems. The importance of reducing mass in the power system is described in terms of the tradeoff with communications systems mass to maximize communications revenue. It surveys solar array components and subsystems to meet these future requirements and attempts to identify the development candidates with a large payoff potential and a high probability of successful development.     

A review of long drop tubes as a supplement/alternative to space experiments

The 100 meter high drop tube at the Marshall Space Flight Center has proven to be a viable facility for studies of containerless solidification. Advantages are that experiments are inexpensive and large numbers of specimens can be processed rapidly. It would not be unusual to run ten specimens in a day. Another significant advantage is that the undercooling behavior can be followed with sufficient sensitivity to easily detect the onset of recalescence and subsequent events.Disadvantages are the restrictions on specimen sizes and types of alloys that can be run in a microgravity environment. Practical specimen sizes range between 50 mg and 500 mg depending on the type of furnace being used. Refractory alloys can be processed in a vacuum (about 10^?5 torr) and therefore at microgravity. Non-refractory alloys demand an atmosphere (about 200 torr) to obtain appreciable undercooling before impact at the bottom of the tube. Under these conditions significant g forces result.Because of the present limitations of the 100 meter drop tube, the most definitive work has been done on niobium based alloys. Large amounts of undercooling have been observed routinely and the effects of undercooling on microstructure have been characterized in detail. X-ray diffraction, scanning electron microscopy, and transmission electron microscopy have been used to determine types of phases, amounts of phases, and compositions of phases. It is clear, as would be expected, that the results bear some resemblance to rapid solidification processing by quenching. However, there are dissimilarities due to the uniqueness of solidification by deep undercooling without quenching in long drop tubes and accompanying recalescence effects.     

Saturn's E ring

The E ring is a loose collection of debris orbiting Saturn well outside the classical ring system. Compared to the classical ring system, the E ring is extraordinarily tenuous but occupies an enormous volume. The optical depth has a pronounced maximum near the orbit of Enceladus, which is therefore widely assumed to be the source of ring material. Optical properties of the ring suggest a collection of micron-sized spheroids whose composition is generally supposed to be water ice in accordance with the assumed Enceladus source.Apart from its unusual physical properties, the very existence of the E ring as a permanent feature of the Saturn system is problematical. The particles of the ring are susceptible to erosion by ion sputtering and outward transport by ion drag. Lifetimes against either of these processes in the presence of observed ion populations are estimated to be no more than a few thousand years, and it is not clear that Enceladus can replenish the ring particles continuously on such a time scale. It has thus been suggested that the E ring is a transient feature of the Saturn system, resulting from an episodic disturbance of Enceladus (e.g., a sizeable meteoroid impact or tectonic event) in the recent past. In any case, the E ring presently provides a convenient natural probe of magnetospheric transport processes because it presents a marginally significant cross section for the absorption of magnetospherically-trapped particles traversing its volume.     

Ralicon anodes fabricated by electron beam lithography for image photon counting detectors

The Anger wedge and strip anode event location system developed for microchannel plate image photon detectors at the Space Sciences Laboratory of the University of California, Berkeley, has been extended in the present work by the use of electron beam lithography (EBL). Computer-aided design methods have been used to develop several types of RALICON ($\text{R}$eadout $\text{A}$nodes of $\text{Lit}$hographic $\text{Con}$struction) for use in photon counting microchannel plate imaging detectors. These anodes are suitable for linear, two dimensional or radial position measurements and they incorporate novel design features made possible by the EBL fabrication technique which significantly extend their application relative to published wedge-strip anode designs.     

Comparative neutral composition instrumentation and new results

Composition and gas density measurement at all altitudes in the atmospheres of earth and other planets are made by mass spectrometers. Because of the impartiality and large dynamic range they are particularly favored for exploratory missions. Measurements of trace constituents, inert gases and height profiles, especially below clouds, are objectives where mass spectrometry is most useful. Significant advances have been made in recent years in development of light weight automated instruments. Experiments conducted in rarified atmospheres have been more successful or results were less controversial than in attempts to analyze high pressure atmospheres. Gas sampling and conditioning techniques are highly specific because of measurement environments and engineering constraints on the mission, and are usually the most critical elements in the experiment. Chemical sample enrichment and scrubbing for noble gas enhancement are additional sample conditioning techniques now employed. Dynamic range of more than 10⁸ is achievable. Reliable measurements of complex organic or chemically active trace constituents with mixing ratios of less than 10^?9 still require significant instrument development particularly where weight, power and sampling time are severely restricted. Adaptation of familiar and proven laboratory techniques for flight instruments is usually not straightforward and practical.     

Software Maintenance Criteria for Small Microprocessor-Based Systems

Criteria is provided for designing and implementing small microprocessor-based programs with the objective of enhancing their maintainability properties. Since microprocessor-based programs typically have relatively short schedules and low budgets. it is necessary to adapt maintainability enhancing practices for these projects in such a manner that their impact in these critical areas is not excessive. Since many of the practices which can be used to enhance maintainability actually coincide with those which improve productivity during software development, application of those practices should incur little or no added cost or duration to a microprocessor-based program. This paper suggests practical steps which can be applied to the software design and implementation activities, to the software documentation, and to the development environment which will enhance the maintainability of the resultant computer programs.     

Analysis of gravity-wave induced instabilities and turbulence viscosity parameters from optical emissions

The effects of gravity waves on OH and 5577 0I emissions have been used to investigate (1) the possible production of “secondary” waves or “ripples” by a “primary” wave; (2) the possible application of such observed optical emissions for computing atmospheric parameters such as turbulence viscosity in the mesospheric regions.