Gravity effects on connective tissue biosynthesis by cultured mesenchymal cells.

Quantitative and qualitative aspects of collagen synthesis under microgravity, normal gravity and hypergravity conditions were investigated during the spacelab D-2 mission by incubating human fibroblast cultures with [3H]-proline for 0, 4, 7, 10 and 20 hours. Quantitative analysis revealed an increase of collagen synthesis under microgravity conditions, being 40% higher than 1g controls. Hypergravity samples at 1.44g, 6.6g and 10g showed a decrease in collagen synthesis with increasing g, being down to about 15% at 10g. The relative proportion of collagen from total protein synthesized, the secretion of collagen by the cells, proline hydroxylation of individual collagen alpha-chains and the relative proportions of collagens I, III and V synthesized were not affected at any of the applied conditions.     

Spectroscopic Measurement of Coronal Compositions

Although the elemental composition in all parts of the solar photosphere appears to be the same this is clearly not the case with the solar upper atmosphere (SUA). Spectroscopic studies show that in the corona elemental composition along solar equatorial regions is usually different from polar regions; composition in quiet Sun regions is often different from coronal hole and active region compositions and the transition region composition is frequently different from the coronal composition along the same line of sight. In the following two issues are discussed. The first involves abundance ratios between the high-FIP O and Ne and the low-FIP Mg and Fe that are important for meaningful comparisons between photospheric and SUA compositions and the second involves a review of composition and time variability of SUA plasmas at heights of 1.0≤h≤1.5R _⊙.     

Space Shuttle UHF communications performance evaluation

Computational investigations and experimental measurements were performed to evaluate the Space Shuttle UHF communication system performance for payload bay antenna at the proposed new location. To insure adequate communication coverage at relocated new location, the link margin for the Extravehicular Activity (EVA) astronauts and between Space Shuttle Orbiter and International Space Station (SSO-ISS) during rendezvous were analyzed. The multipath effects from the vehicle structures surrounding the antenna were investigated. The Radio Frequency (RF) electromagnetic radiation to the Orbiter Docking System (ODS) pyrotechnics was also analyzed to ensure the EMC/EMI compliances.     

Road profile recognition for autonomous car navigation and Navstar GPS support

We discuss autonomous car navigation based on updating dead reckoning (DR) by road profile recognition (RPR). The navigation system requires sensors to detect changes in altitude and driving direction which are installed in modern cars for different purposes (e.g. ABS sensors). The layout of the navigation system is discussed and simulations are carried out over driving distances of approximately 150 km on the basis of realistic road data and ordinary sensor accuracies. Positioning errors of lower than 10 m (standard deviation) are observed. To achieve this accuracy the synchronization error between measured and mapped data must be continually estimated. The introduced navigation method is ideal to complete present commercial car navigation systems using Navstar GPS.     

Biological responses to space: results of the experiment "Exobiological Unit" of ERA on EURECA I.

Spores of different strains of Bacillus subtilis and the Escherichia coli plasmid pUC19 were exposed to selected conditions of space (space vacuum and/or defined wavebands and intensities of solar ultraviolet radiation) in the experiment ER 161 "Exobiological Unit" of the Exobiology Radiation Assembly (ERA) on board of the European Retrievable Carrier (EURECA). After the approximately 11 months lasting mission, their responses were studied in terms of survival, mutagenesis in the his (B. subtilis) or lac locus (pUC19), induction of DNA strand breaks, efficiency of DNA repair systems, and the role of external protective agents. The data were compared with those of a simultaneously running ground control experiment. The survival of spores treated with the vacuum of space, however shielded against solar radiation, is substantially increased, if they are exposed in multilayers and/or in the presence of glucose as protective, whereas all spores in "artificial meteorites", i.e. embedded in clays or simulated Martian soil, are killed. Vacuum treatment leads to an increase of mutation frequency in spores, but not in plasmid DNA. Extraterrestrial solar ultraviolet radiation is mutagenic, induces strand breaks in the DNA and reduces survival substantially; however, even at the highest fluences, i.e. 3 x 10(8) J m-2, a small but significant fraction of spores survives the insolation. Action spectroscopy confirms results of previous space experiments of a synergistic action of space vacuum and solar UV radiation with DNA being the critical target.     

Experimental Aspects of Mid-Frequency Pulsations (f≈10–100 mHz) in the Southern Polar Cap

We review the results obtained in the frequency range of Pc3 (22-100 mHz) and Pc4 (7-22 mHz) pulsations at Italian Antarctic stations in the southern polar cap (“Mario Zucchelli”, at Terra Nova Bay, TNB, 80^˚.S; “Concordia”, the Italian/French base at Dome C, DMC, 89^˚.S). The absence of a midnight enhancement in the pulsation power suggests a negligible substorm influence at extreme latitudes, while the sharp noon enhancement, which appears only at TNB, is determined by the closer proximity of the station to cusp related phenomena. The relationship between the frequency of the band-limited signals and the interplanetary magnetic field strength, the cone angle influence, and the higher correlation of the Pc3 power with the solar wind speed in the morning hours suggest a global scenario in which upstream waves would be mainly responsible for the mid-frequency activity in the polar cap. However, the polarization pattern is odd with respect to the predictions for tailward propagating modes.     

Filtering of discontinuous processes arising in marine integratednavigation systems

A refined stochastic model for the errors of the Loran-C radio navigation aid is described, and it is shown how this model can be used to improve the performance of integrated navigation systems. In addition to the usual propagation errors, Loran-C time of arrival measurements are occasionally plagued with sudden intermittent errors of a particular magnitude and caused by receiver cycle selection errors. These result in sudden large jumps in the calculated position solution. The Loran-C error has been modeled as the sum of a diffusion process, representing the normal propagating errors, and a pure jump process of Poisson type, representing the cycle selection errors. A simple integrated navigation system is then described, based on the Loran-C model and the standard dead reckoning (heading and speed) system model. Assuming that the observed process is governed by a linear stochastic difference equation, a recursive linear unbiased minimum variance filter is developed, from which the Loran-C and dead reckoning errors, and hence position and velocity, can be estimated     

The Plasma Environment of Comet 67P/Churyumov-Gerasimenko Throughout the Rosetta Main Mission

The plasma environment of comet 67P/Churyumov-Gerasimenko, the Rosetta mission target comet, is explored over a range of heliocentric distances throughout the mission: 3.25 AU (Rosetta instruments on), 2.7 AU (Lander down), 2.0 AU, and 1.3 AU (perihelion). Because of the large range of gas production rates, we have used both a fluid-based magnetohydrodynamic (MHD) model as well as a semi-kinetic hybrid particle model to study the plasma distribution. We describe the variation in plasma environs over the mission as well as the differences between the two modeling approaches under different conditions. In addition, we present results from a field aligned, two-stream transport electron model of the suprathermal electron flux when the comet is near perihelion.     

A regional tropospheric model for airborne GPS applications

A regional tropospheric model can be constructed using surveys from GPS ground networks. Using this model the tropospheric delays of a kinematic station within the region can be interpolated. However, such a model is generally not suitable for an airborne platform high above the ground networks. In this paper, a method of constructing a regional tropospheric model for airborne GPS applications is described. First, the kinematic station in the air is projected onto the ground. Then the tropospheric delays at projected point are interpolated from those of the ground networks. Finally, the tropospheric delays at projected point are extended upward to the airborne platform using pressure and temperature gradients and humidity exponential function. For validation of this method, the data of airborne campaign carried out by BKG in cooperation with GFZ and BGR 2008 in the northern Alps are used. The results show that GPS kinematic positioning precision in height component can be improved using this method.     

Fuel Savings in the WHECON Attitude Control System Using an Asymmetric Controller

This paper investigates the possibility of generating an on-off limit cycle mode in the WHECON attitude control system. lt is shown that, by eliminating the off set angle ? in the roll-actuated control torquer, the WHECON system can be reduced to the structure considered earlier by Mendel and, therefore, an on-off limit cycle can be generated simply by introducing an appropriate amount of asymmetry into the deadzone characteristic of the WHECON controller. Because with this mode of on-off limit cycle the thruster jets will fire only once in each cycle of the oscillation, it is claimed that a considerable amount of fuel can be saved by the proposed modifications to the original WHECON design. Dynamic simulation study was conducted with the actual nonlinear model of the WHECON system considered and the results indicate that, for both zero and nonzero values of the off set angle ?, substantial fuel savings can be achieved by using the asymmetric controller.