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171.
Chromosome aberrations as biomarkers of radiation exposure: modelling basic mechanisms. 总被引:1,自引:0,他引:1
F Ballarini A Ottolenghi 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,31(6):1557-1568
The space radiation environment is a mixed field consisting of different particles having different energies, including high charge and energy (HZE) ions. Conventional measurements of absorbed doses may not be sufficient to completely characterise the radiation field and perform reliable estimates of health risks. Biological dosimetry, based on the observation of specific radiation-induced endpoints (typically chromosome aberrations), can be a helpful approach in case of monitored exposure to space radiation or other mixed fields, as well as in case of accidental exposure. Furthermore, various ratios of aberrations (e.g. dicentric chromosomes to centric rings and complex exchanges to simple exchanges) have been suggested as possible fingerprints of radiation quality, although all of them have been subjected to some criticisms. In this context a mechanistic model and a Monte Carlo code for the simulation of chromosome aberration induction were developed. The model, able to provide dose-responses for different aberrations (e.g. dicentrics, rings, fragments, translocations, insertions and other complex exchanges), was further developed to assess the dependence of various ratios of aberrations on radiation quality. The predictions of the model were compared with available data, whose experimental conditions were faithfully reproduced. Particular attention was devoted to the scoring criteria adopted in different laboratories and to possible biases introduced by interphase death and mitotic delay. This latter aspect was investigated by taking into account both metaphase data and data obtained with Premature Chromosome Condensation (PCC). 相似文献
172.
Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation. 总被引:1,自引:0,他引:1
C Baumstark-Khan J Heilmann H Rink 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,31(6):1583-1591
The lens epithelium is the initiation site for the development of radiation induced cataracts. Radiation in the cortex and nucleus interacts with proteins, while in the epithelium, experimental results reveal mutagenic and cytotoxic effects. It is suggested that incorrectly repaired DNA damage may be lethal in terms of cellular reproduction and also may initiate the development of mutations or transformations in surviving cells. The occurrence of such genetically modified cells may lead to lens opacification. For a quantitative risk estimation for astronauts and space travelers it is necessary to know the relative biological effectiveness (RBE), because the spacial and temporal distribution of initial physical damage induced by cosmic radiation differ significantly from that of X-rays. RBEs for the induction of DNA strand breaks and the efficiency of repair of these breaks were measured in cultured diploid bovine lens epithelial cells exposed to different LET irradiation to either 300 kV X-rays or to heavy ions at the UNILAC accelerator at GSI. Accelerated ions from Z=8 (O) to Z=92 (U) were used. Strand breaks were measured by hydroxyapatite chromatography of alkaline unwound DNA (overall strand breaks). Results showed that DNA damage occurs as a function of dose, of kinetic energy and of LET. For particles having the same LET the severity of the DNA damage increases with dose. For a given particle dose, as the LET rises, the numbers of DNA strand breaks increase to a maximum and then reach a plateau or decrease. Repair kinetics depend on the fluence (irradiation dose). At any LET value, repair is much slower after heavy ion exposure than after X-irradiation. For ions with an LET of less than 10,000 keV micrometers-1 more than 90 percent of the strand breaks induced are repaired within 24 hours. At higher particle fluences, especially for low energetic particles with a very high local density of energy deposition within the particle track, a higher proportion of non-rejoined breaks is found, even after prolonged periods of incubation. At the highest LET value (16,300 keV micrometers-1) no significant repair is observed. These LET-dependencies are consistent with the current mechanistic model for radiation induced cataractogenesis which postulates that genomic damage to the surviving fraction of epithelial cells is responsible for lens opacification. 相似文献
173.
R. Schmidt H. Arends W. Riedler K. Torkar F. Rüdenauer M. Fehringer B. Maehlum B. Narheim 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1992,12(12):61-64
Future space missions aiming at the accurate measurement of cold plasmas and DC to very low frequency electric fields will require that the potential of their conductive surfaces be actively controlled to be near the ambient plasma potential. In the near-Earth space these spacecraft are usually solar-cell powered; consequently, parts of their surface are most of the time exposed to solar photons. Outside the plasmasphere, a positive surface potential due the dominance of surface-emitted photoelectrons over ambient plasma electrons is to be expected. Photo- and ambient electrons largely determine the potential and positive values between a few Volts up to 100 V have been observed. Active ion emission is the obvious solution of this problem. A liquid metal ion emitter and a saddle field ion emitter are nearing the stage of flight unit fabrication. We will attempt to clamp the spacecraft potential to values close to the plasma potential. We present first results from vacuum chamber tests and describe the emission behaviour and characteristics of emitters producing, respectively, In+ and N2+ beams with an energy of ≥ 5 keV. 相似文献
174.
Long-term survival of bacterial spores in space. 总被引:8,自引:0,他引:8
G Horneck H Bucker G Reitz 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1994,14(10):41-45
On board of the NASA Long Duration Exposure Facility (LDEF), spores of Bacillus subtilis in monolayers (10(6)/sample) or multilayers (10(8)/sample) were exposed to the space environment for nearly six years and their survival was analyzed after retrieval. The response to space parameters, such as vacuum (10(-6) Pa), solar electromagnetic radiation up to the highly energetic vacuum-ultraviolet range (10(9) J/m2) and/or cosmic radiation (4.8 Gy), was studied and compared to the results of a simultaneously running ground control experiment. If shielded against solar ultraviolet (UV)-radiation, up to 80 % of spores in multilayers survive in space. Solar UV-radiation, being the most deleterious parameter of space, reduces survival by 4 orders of magnitude or more. However, up to 10(4) viable spores were still recovered, even in completely unprotected samples. Substances, such as glucose or buffer salts serve as chemical protectants. With this 6 year study in space, experimental data are provided to the discussion on the likelihood of "Panspermia". 相似文献
175.
Annual solar UV exposure and biological effective dose rates on the Martian surface. 总被引:4,自引:0,他引:4
M R Patel A Bérces T Kerékgyárto Gy Rontó H Lammer J C Zarnecki 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2004,33(8):1247-1252
The ultraviolet (UV) environment of Mars has been investigated to gain an understanding of the variation of exposure throughout a Martian year, and link this flux to biological effects and possible survival of organisms at the Martian surface. To gain an idea of how the solar UV radiation varies between different regions, including planned landing sites of two future Mars surface missions, we modelled the total solar UV surface flux throughout one Martian year for two different dust scenarios. To understand the degree of solar UV stress on micro-organisms and/or molecules essential for life on the surface of Mars, we also calculated the biologically effective dose (BED) for T7 and Uracil in relevant wavelength regions at the Martian surface as a function of season and latitude, and discuss the biological survival rates in the presence of Martian solar UV radiation. High T7/Uracil BED ratios indicate that even at high latitudes where the UV flux is significantly reduced, the radiation environment is still hostile for life due to the persisting UV-C component of the flux. 相似文献
176.
T.L. Zhang H. Zhao C.T. Russell S.M. Petrinec K. Schwingenschun W. Riedler 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1997,19(12):1943-1946
The northward and southward orientation of the interplanetary magnetic field (IMF) is usually considered as providing the external boundary conditions in the solar wind interaction with the Earth's magnetopause but it is the magnetic field in the magnetosheath that interacts with the Earth's magnetic field. In this paper, we consider the possibility that the wave activity in the foreshock region may affect the magnetic field orientation in the magnetosheath with time scales that might be geomagnetically effective. If magnetosheath magnetic field becomes disturbed on plasma streamlines which are connected to the quasi-parallel bow shock and foreshock, the magnetic field orientation on the inner magnetosheath may differ significantly from the undisturbed IMF. We present a model of dayside reconnection which may occur when the IMF northward and illustrate its effects on the erosion of the magnetopause. 相似文献
177.
V Bluem F Paris 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,31(1):77-86
Water is the essential precondition of life in general and also for the establishment of a Martian base suitable for long duration stays of humans. It is not yet proven if there is indeed a "frozen ocean" under the surface of Mars but if this could be verified it would open innovative aspects for the construction of bioregenerative life support systems (BLSS). In a general concept higher plants will play the predominant role in a Martian BLSS. It is not clear, however, how these will grow and bring seed in reduced gravity and there may be differences in the productivity in comparison to Earth conditions. Therefore, organisms which are already adapted to low gravity conditions, namely non-gravitropic aquatic plants and also aquatic animals may be used to enhance the functionality of the Martian BLSS as a whole. It has been shown already with the so-called C.E.B.A.S. MINIMODULE in the STS-89 and STS-90 spaceshuttle missions that the water plant Ceratophyllum demersum has an undisturbed and high biomass production under space conditions. Moreover, the teleost fish species Xiphophorus helleri adapted easily to the micro-g environment and maintained its normal reproductive functions. Based on this findings a possible scenario is presented in which aquatic plant production modules and combined animal-plant production systems may be used for human food production and water and air regeneration in a Martian base. 相似文献
178.
D Voeste L H Levine H G Levine V Blum 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2003,31(1):211-214
The Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) Mini-Module, a Space Shuttle middeck locker payload which supports a variety of aquatic inhabitants (fish, snails, plants and bacteria) in an enclosed 8.6 L chamber, was tested for its biological stability in microgravity. The aquatic plant, Ceratophyllum demersum L., was critical for the vitality and functioning of this artificial mini-ecosystem. Its photosynthetic pigment concentrations were of interest due to their light harvesting and protective functions. "Post-flight" chlorophyll and carotenoid concentrations within Ceratophyllum apical segments were directly related to the quantities of light received in the experiments, with microgravity exposure (STS-89) failing to account for any significant deviation from ground control studies. 相似文献
179.
The effect of gravity on surface temperature and net photosynthetic rate of plant leaves. 总被引:3,自引:0,他引:3
Y Kitaya M Kawai J Tsuruyama H Takahashi A Tani E Goto T Saito M Kiyota 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》2001,28(4):659-664
To clarify the effects of gravity on heat/gas exchange between plant leaves and the ambient air, the leaf temperatures and net photosynthetic rates of plant leaves were evaluated at 0.01, 1.0, 1.5 and 2.0 G of 20 seconds each during a parabolic airplane flight. Thermal images of leaves were captured using infrared thermography at an air temperature of 26 degrees C, a relative humidity of 15% and an irradiance of 260 W m-2. The net photosynthetic rates were determined by using a chamber method with an infrared gas analyzer at an air temperature of 20 degrees C, a relative humidity of 50% and a photosynthetic photon flux of 0.5 mmol m-2 s-1. The mean leaf temperature increased by 1 degree C and the net photosynthetic rate decreased by 13% with decreasing gravity levels from 1.0 to 0.01 G. The leaf temperature decreased by 0.5 degree C and the net photosynthetic rate increased by 7% with increasing gravity levels from 1.0 to 2.0 G. Heat/gas exchanges between leaves and the ambient air were more retarded at lower gravity levels. A restricted free air convection under microgravity conditions in space would limit plant growth by retarding heat and gas exchanges between leaves and the ambient air. 相似文献
180.
J L Garland C L Mackowiak R F Strayer B W Finger 《Advances in Space Research (includes Cospar's Information Bulletin, Space Research Today)》1997,20(10):1821-1826
After initial emphasis on large-scale baseline crop tests, the Kennedy Space Center (KSC) Breadboard project has begun to evaluate long-term operation of the biomass production system with increasing material closure. Our goal is to define the minimum biological processing necessary to make waste streams compatible with plant growth in hydroponic systems, thereby recycling nutrients into plant biomass and recovering water via atmospheric condensate. Initial small and intermediate-scale studies focused on the recycling of nutrients contained in inedible plant biomass. Studies conducted between 1989-1992 indicated that the majority of nutrients could be rapidly solubilized in water, but the direct use of this crop "leachate" was deleterious to plant growth due to the presence of soluble organic compounds. Subsequent studies at both the intermediate scale and in the large-scale Biomass Production Chamber (BPC) have indicated that aerobic microbiological processing of crop residue prior to incorporation into recirculating hydroponic solutions eliminated any phytotoxic effect, even when the majority of the plant nutrient demand was provided from recycled biomass during long term studies (i.e. up to 418 days). Current and future studies are focused on optimizing biological processing of both plant and human waste streams. 相似文献