An analysis of low-frequency component in microacceleration measurements made onboard the <Emphasis Type="Italic">Foton M-2</Emphasis> satellite

The low-frequency component is investigated in the data of measurements performed onboard the Foton M-2 satellite with the three-component accelerometer TAS-3. Investigations consisted in comparison of this component with its calculated analog found from a reconstruction of the satellite’s attitude motion. The influence of the Earth’s magnetic field on the accelerometer readings is discovered by way of spectral analysis of the functions representing the results of determining the low-frequency microacceleration by two methods. After making correction for this influence, the results obtained by these two methods coincided within a root-mean-square error of less than 10^?6 m/s².     

Electric currents from thunderstorms to the ionosphere during a solar cycle: Quasi-static modeling of the coupling mechanism

Conduction and displacement currents, and their sum the Maxwell current, generated over a thunderstorm (TS) with recurrent lightning discharges are investigated theoretically. The aim is to study better the influence of different factors on these currents, which form the link between thunderstorms and the ionosphere in the global atmospheric electrical circuit. The factors studied concern the thunderstorm characteristics (the charge separation current, and the lightning discharge parameters), as well as the atmospheric and cloud conductivity. Some of these factors may show long-term changes with the 11-year solar cycle, possibly realized through an inverse dependence of the cosmic ray flux on solar activity. Earlier investigations have suggested that the lightning-related charge redistribution and subsequent relaxation, rather than the high intensity current, is mainly the source of the energy coupled to the ionosphere. With respect to this, a quasi-electrostatic analytical model is proposed, based on Maxwell’s equations. The currents are generated by a TS modeled as a positive vertical dipole with charges which are first accumulated and then destroyed by lightning. Our computations show that the mean and peak values of the conduction and total Maxwell currents to the ionosphere depend significantly on the charge moment change. The mean currents are also sensitive to the reduction of the conductivity in thunderclouds. Small variations of the stratospheric conductivity (20% at geomagnetic latitude 40° and 40–50% at 55°) with the solar activity do not influence the currents to the ionosphere very much.     

Host–parasite interactions in closed and open microbial cultivation system

The study addresses interaction of bacteria and phages in the host–parasite system in batch and continuous cultures. The study system consists of the auxotrophic strain of Brevibacterium – Brevibacterium sp. 22L – and the bacteriophage of Brevibacterium sp., isolated from the soil by the enrichment method.

1.

Closed system. In the investigation of the relationship between the time of bacterial lysis and multiplicity of phage infection it has been found that at a lower phage amount per cell it takes a longer time for the lysis of the culture to become discernible. Another important factor determining cytolysis in liquid medium is the physiological state of bacterial population. Specific growth rate of bacteria at the moment of phage infection has been chosen as an indicator of the physiological state of bacteria. It has been shown that the shortest latent period and the largest output of the phage are observed during the logarithmic growth phase of bacteria grown under favorable nutrient conditions. In the stationary phase, bacterial cells become “a bad host” for the phage, whose reproduction rate decreases, and the lysis either slows down significantly or does not occur at all.     

Which solar EUV indices are best for reconstructing the solar EUV irradiance?

The solar EUV irradiance is of key importance for space weather. Most of the time, however, surrogate quantities such as EUV indices have to be used by lack of continuous and spectrally resolved measurements of the irradiance. The ability of such proxies to reproduce the irradiance from different solar atmospheric layers is usually investigated by comparing patterns of temporal correlations. We consider instead a statistical approach. The TIMED/SEE experiment, which has been continuously operating since February 2002, allows for the first time to compare in a statistical manner the EUV spectral irradiance to five EUV proxies: the sunspot number, the f10.7, Ca K, and Mg II indices, and the He I equivalent width.     

Current status of X-ray spectrometer development in the SELENE project

The X-ray spectrometer (XRS) on the SELENE (SELenological and ENgineering Explorer) spacecraft, XRS, will observe fluorescent X-rays from the lunar surface. The energy of the fluorescent X-ray depends on the elements of which the lunar soil consists, therefore we can determine elemental composition of the upper most lunar surface. The XRS consists of three components: XRF-A, SOL-B, and SOL-C. XRF-A is the main sensor to observe X-rays from the lunar surface. SOL-B is direct monitor of Solar X-ray using Si-PIN photodiode. SOL-C is another Solar X-ray monitor but observes the X-rays from the standard sample attached on the base plate. This enables us to analyze by a comparative method similar to typical laboratory XRF methods. XRF-A and SOL-C adopt charge coupled device as an X-ray detector which depletion layer is deep enough to detect X-rays. The X-ray spectra were obtained by the flight model of XRS components, and all components has been worked well to analyze fluorescent X-rays. Currently, development of the hardware and software of the XRS has been finished and we are preparing for system integration test for the launch.     

Presynaptic transporter-mediated release of glutamate evoked by the protonophore FCCP increases under altered gravity conditions

High-affinity Na⁺-dependent glutamate transporters of the plasma membrane mediate the glutamate uptake into neurons, and thus maintain low levels of extracellular glutamate in the synaptic cleft. The study focused on the release of glutamate by reversal of Na⁺-dependent glutamate transporters from rat brain nerve terminals (synaptosomes) under conditions of centrifuge-induced hypergravity. Flow cytometric analysis revealed similarity in the size and cytoplasmic granularity between synaptosomal preparations obtained from control and G-loaded animals (10 G, 1 h). The release of cytosolic l-[¹⁴C]glutamate from synaptosomes was evaluated using the protonophore FCCP, which dissipated synaptic vesicle proton gradient, thus synaptic vesicles were not able to keep glutamate inside and the latter enriched cytosol. FCCP per se induced the greater release of l-[¹⁴C]glutamate in hypergravity as compared to control (4.8 ± 1.0% and 8.0 ± 1.0% of total label). Exocytotic release of l-[¹⁴C]glutamate evoked by depolarization was reduced down to zero after FCCP application under both conditions studied. Depolarization stimulated release of cytosolic l-[¹⁴C]glutamate from synaptosomes preliminary treated with FCCP was considerably increased from 27.0 ± 2.2% of total label in control to 35.0 ± 2.3% in hypergravity. Non-transportable inhibitor of glutamate transporter dl-threo-β-benzyloxyaspartate was found to significantly inhibit high-KCl and FCCP-stimulated release of l-[¹⁴C]glutamate, confirming the release by reversal of glutamate transporters. The enhancement of transporter-mediated release of glutamate in hypergravity was found to result at least partially from the inhibition of the activity of Na/K-ATPase in the plasma membrane of synaptosomes. We suggested that hypergravity-induced alteration in transporter-mediated release of glutamate indicated hypoxic injury of neurons.     

Fluorescence microscopy as a tool for in situ life detection

The identification of extant and, in some cases, extinct bacterial life is most convincingly and efficiently performed with modern high-resolution microscopy. Epifluorescence microscopy of microbial autofluorescence or in conjunction with fluorescent dyes is among the most useful of these techniques. We explored fluorescent labeling and imaging of bacteria in rock and soil in the context of in situ life detection for planetary exploration. The goals were two-fold: to target non-Earth-centric biosignatures with the greatest possible sensitivity and to develop labeling procedures amenable to robotic implementation with technologies that are currently space qualified. A wide panel of commercially available dyes that target specific biosignature molecules was screened, and those with desirable properties (i.e., minimal binding to minerals, strong autofluorescence contrast, no need for wash steps) were identified. We also explored the potential of semiconductor quantum dots (QDs) as bacterial and space probes. A specific instrument for space implementation is suggested and discussed.     

Some ecological mechanisms to generate habitability in planetary subsurface areas by chemolithotrophic communities: the Río Tinto subsurface ecosystem as a model system

Chemolithotrophic communities that colonize subsurface habitats have great relevance for the astrobiological exploration of our Solar System. We hypothesize that the chemical and thermal stabilization of an environment through microbial activity could make a given planetary region habitable. The MARTE project ground-truth drilling campaigns that sampled cryptic subsurface microbial communities in the basement of the Río Tinto headwaters have shown that acidic surficial habitats are the result of the microbial oxidation of pyritic ores. The oxidation process is exothermic and releases heat under both aerobic and anaerobic conditions. These microbial communities can maintain the subsurface habitat temperature through storage heat if the subsurface temperature does not exceed their maximum growth temperature. In the acidic solutions of the Río Tinto, ferric iron acts as an effective buffer for controlling water pH. Under anaerobic conditions, ferric iron is the oxidant used by microbes to decompose pyrite through the production of sulfate, ferrous iron, and protons. The integration between the physical and chemical processes mediated by microorganisms with those driven by the local geology and hydrology have led us to hypothesize that thermal and chemical regulation mechanisms exist in this environment and that these homeostatic mechanisms could play an essential role in creating habitable areas for other types of microorganisms. Therefore, searching for the physicochemical expression of extinct and extant homeostatic mechanisms through physical and chemical anomalies in the Mars crust (i.e., local thermal gradient or high concentration of unusual products such as ferric sulfates precipitated out from acidic solutions produced by hypothetical microbial communities) could be a first step in the search for biological traces of a putative extant or extinct Mars biosphere.     

The Dynamic Albedo of Neutrons (DAN) experiment for NASA's 2009 Mars Science Laboratory

We present a summary of the physical principles and design of the Dynamic Albedo of Neutrons (DAN) instrument onboard NASA's 2009 Mars Science Laboratory (MSL) mission. The DAN instrument will use the method of neutron-neutron activation analysis in a space application to study the abundance and depth distribution of water in the martian subsurface along the path of the MSL rover.