Presynaptic transporter-mediated release of glutamate evoked by the protonophore FCCP increases under altered gravity conditions

High-affinity Na⁺-dependent glutamate transporters of the plasma membrane mediate the glutamate uptake into neurons, and thus maintain low levels of extracellular glutamate in the synaptic cleft. The study focused on the release of glutamate by reversal of Na⁺-dependent glutamate transporters from rat brain nerve terminals (synaptosomes) under conditions of centrifuge-induced hypergravity. Flow cytometric analysis revealed similarity in the size and cytoplasmic granularity between synaptosomal preparations obtained from control and G-loaded animals (10 G, 1 h). The release of cytosolic l-[¹⁴C]glutamate from synaptosomes was evaluated using the protonophore FCCP, which dissipated synaptic vesicle proton gradient, thus synaptic vesicles were not able to keep glutamate inside and the latter enriched cytosol. FCCP per se induced the greater release of l-[¹⁴C]glutamate in hypergravity as compared to control (4.8 ± 1.0% and 8.0 ± 1.0% of total label). Exocytotic release of l-[¹⁴C]glutamate evoked by depolarization was reduced down to zero after FCCP application under both conditions studied. Depolarization stimulated release of cytosolic l-[¹⁴C]glutamate from synaptosomes preliminary treated with FCCP was considerably increased from 27.0 ± 2.2% of total label in control to 35.0 ± 2.3% in hypergravity. Non-transportable inhibitor of glutamate transporter dl-threo-β-benzyloxyaspartate was found to significantly inhibit high-KCl and FCCP-stimulated release of l-[¹⁴C]glutamate, confirming the release by reversal of glutamate transporters. The enhancement of transporter-mediated release of glutamate in hypergravity was found to result at least partially from the inhibition of the activity of Na/K-ATPase in the plasma membrane of synaptosomes. We suggested that hypergravity-induced alteration in transporter-mediated release of glutamate indicated hypoxic injury of neurons.     

Fluorescence microscopy as a tool for in situ life detection

The identification of extant and, in some cases, extinct bacterial life is most convincingly and efficiently performed with modern high-resolution microscopy. Epifluorescence microscopy of microbial autofluorescence or in conjunction with fluorescent dyes is among the most useful of these techniques. We explored fluorescent labeling and imaging of bacteria in rock and soil in the context of in situ life detection for planetary exploration. The goals were two-fold: to target non-Earth-centric biosignatures with the greatest possible sensitivity and to develop labeling procedures amenable to robotic implementation with technologies that are currently space qualified. A wide panel of commercially available dyes that target specific biosignature molecules was screened, and those with desirable properties (i.e., minimal binding to minerals, strong autofluorescence contrast, no need for wash steps) were identified. We also explored the potential of semiconductor quantum dots (QDs) as bacterial and space probes. A specific instrument for space implementation is suggested and discussed.     

Some ecological mechanisms to generate habitability in planetary subsurface areas by chemolithotrophic communities: the Río Tinto subsurface ecosystem as a model system

Chemolithotrophic communities that colonize subsurface habitats have great relevance for the astrobiological exploration of our Solar System. We hypothesize that the chemical and thermal stabilization of an environment through microbial activity could make a given planetary region habitable. The MARTE project ground-truth drilling campaigns that sampled cryptic subsurface microbial communities in the basement of the Río Tinto headwaters have shown that acidic surficial habitats are the result of the microbial oxidation of pyritic ores. The oxidation process is exothermic and releases heat under both aerobic and anaerobic conditions. These microbial communities can maintain the subsurface habitat temperature through storage heat if the subsurface temperature does not exceed their maximum growth temperature. In the acidic solutions of the Río Tinto, ferric iron acts as an effective buffer for controlling water pH. Under anaerobic conditions, ferric iron is the oxidant used by microbes to decompose pyrite through the production of sulfate, ferrous iron, and protons. The integration between the physical and chemical processes mediated by microorganisms with those driven by the local geology and hydrology have led us to hypothesize that thermal and chemical regulation mechanisms exist in this environment and that these homeostatic mechanisms could play an essential role in creating habitable areas for other types of microorganisms. Therefore, searching for the physicochemical expression of extinct and extant homeostatic mechanisms through physical and chemical anomalies in the Mars crust (i.e., local thermal gradient or high concentration of unusual products such as ferric sulfates precipitated out from acidic solutions produced by hypothetical microbial communities) could be a first step in the search for biological traces of a putative extant or extinct Mars biosphere.     

The Dynamic Albedo of Neutrons (DAN) experiment for NASA's 2009 Mars Science Laboratory

We present a summary of the physical principles and design of the Dynamic Albedo of Neutrons (DAN) instrument onboard NASA's 2009 Mars Science Laboratory (MSL) mission. The DAN instrument will use the method of neutron-neutron activation analysis in a space application to study the abundance and depth distribution of water in the martian subsurface along the path of the MSL rover.     

Development of a plant growth unit for growing plants over a long-term life cycle under microgravity conditions.

To study the effect of the space environment on plant growth including the reproductive growth and genetic aberration for a long-term plant life cycle, we have initiated development of a new type of facility for growing plants under microgravity conditions. The facility is constructed with subsystems for controlling environmental elements. In this paper, the concept of the facility design is outlined. Subsystems controlling air temperature, humidity, CO2 concentration, light and air circulation around plants and delivering recycled water and nutrients to roots are the major concerns. Plant experiments for developing the facility and future plant experiments with the completed facility are also overviewed. We intend to install this facility in the Japan Experiment Facility (JEM) boarded on the International Space Station.     

The Galaxy Cluster Mass Scale and Its Impact on Cosmological Constraints from the Cluster Population

Space Science Reviews - The QB50 mission is a satellite constellation designed to carry out measurements at between 200–380 km altitude in the ionosphere. The multi-needle Langmuir probe...     

The quiescent spectrum of 2S1636-536

During the EXOSAT observation of 2S1636-536 in July 1983 the quiescent flux was observed to brighten from 1.7×10 ^-9 ergs/cm. ² /sec to 2.6×10^-9 ergs/cm. ² /sec, this increase being accompanied by a decrease in effective temperature. Further analysis of the spectrum reveals that the best fit is a power law with an index which increases from -1.75 to-2.1 while the source brightens; there is some evidence for an upper cut off. This spectrum is interpreted as the result of soft photons from the neutron star surface which are Comptonised in a hot plasma cloud surrounding the neutron star (neutron star corona). The increasing spectral index is seen as the result of Compton cooling of the corona as the output of quiescent flux rises in response to an increasing accretion rate.     

The Venus ionosphere

Physical properties of the Venus ionosphere obtained by experiments on the US Pioneer Venus and the Soviet Venera missions are presented in the form of models suitable for inclusion in the Venus International Reference Atmosphere. The models comprise electron density (from 120 km), electron and ion temperatures, and relative ion abundance in the altitude range from 150 km to 1000 km for solar zenith angles from 0° to 180°. In addition, information on ion transport velocities, ionopause altitudes, and magnetic field characteristics of the Venus ionosphere, are presented in tabular or graphical form. Also discussed is the solar control of the physical properties of the Venus ionosphere.     

Kalman filter mechanization for INS airstart

A strapdown mechanization and associated Kalman filter are developed to provide both ground align and airstart capabilities for inertial navigation systems (INSs) using Doppler velocity and position fixes, while not requiring an initial heading estimate. Position update during coarse mode is possible by defining sine and cosine of wander angle as filter states and modeling the position error in geographic frame while integrating velocity in the wander frame. INS Global Positioning System (GPS) differential position due to GPS antenna moment arm can aid heading convergence during hover turns in helicopter applications. Azimuth error state in the fine mode of the filter is defined as wander angle error to provide continuous estimation of navigational states, as well as inertial/aiding sensor errors, across the coarse-to-fine mode transition. Though motivated by a tactical helicopter application, the design can be applied to other vehicles. Advantages over conventional systems in addition to the airstart capability include robustness and versatility in handling many different operational conditions     

Radiation chemistry of the hippocampal brain slice.

The in vitro hippocampal brain slice is a 0.4 mm thick neural network that can be used to study brain responses to radiation and related injuries. This preparation is unique in that it responds to ionizing radiation within minutes after exposure without complications from changes in vascularity, blood flow, blood pressure, etc. Electrophysiological studies have shown that x- and gamma-rays alter synaptic transmission and spike generation, elements of normal brain activity. To evaluate the role of hydroxyl free radicals (OH) in these changes, slices were exposed to dilute H2O2 solutions. EPR spin trapping experiments verified that OH is produced. Neural responses, while similar, were not identical to those due to radiation, possibly because of a different distribution of OH. Although H2O2 is freely diffusible, it produces OH at specific sites where, e.g. iron reduces it. In contrast, x- and gamma-rays produce OH more uniformly throughout the tissue. H2O2 may provide a better model for high-LET radiation where yields of radical products of water radiolysis are decreased and peroxide reactions predominate.