Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

Biosphere 2 Test Module: a ground-based sunlight-driven prototype of a closed ecological life support system.

Constructed in 1986, the Biosphere 2 Test Module has been used since the end of that year for closed ecological systems experiments. It is the largest closed ecological facility ever built, with a sealed variable volume of some 480 cubic meters. It is built with a skin of steel spaceframes with double-laminated glass panels admitting about 65 percent Photosynthetically Active Radiation (PAR). The floor is of welded steel and there is an underground atmospheric connection via an air duct to a variable volume chamber ("lung") permitting expansion and contraction of the Test Module's air volume caused by changes in temperature and barometric pressure, which causes a slight positive pressure from inside the closed system to the outside thereby insuring that the very small leakage rate is outward. Several series of closed ecological system investigations have been carried out in this facility. One series of experiments investigated the dynamics of higher plants and associated soils with the atmosphere under varying light and temperature conditions. Another series of experiments included one human in the closed system for three, five and twenty-one days. During these experiments the Test Module had subsystems which completely recycled its water and atmosphere; all the human dietary needs were produced within the facility, and all wastes were recycled using a marsh plant/microbe system. Other experiments have examined the capability of individual component systems used, such as the soil bed reactors, to eliminate experimentally introduced trace gases. Analytic systems developed for these experiments include continuous monitors of eleven atmospheric gases in addition to the complete gas chromatography mass spectrometry (GCMS) examinations of potable, waste system and irrigation water quality.     

Simple solutions for hyperbolic and related position fixes

Navigation fixed from range differences to three stations and an additional piece of information are investigated. It is shown that if the additional information is the navigator altitude, or the range difference to a fourth station, the computation of the navigation fix is reduced to finding the roots of a quadratic. If the additional information is the range to another station, or that the navigator is on the Earth ellipsoid, the fix can be obtained by solving a quartic. By emphasizing the underlying geometric interpretations, these fixes and their simple solutions are made clear. The derivations also show that the same solution algorithms are applicable if the basic navigation measurements are range sums instead of range differences     

CELSS nutrition system utilizing snails

At the 40th IAF Congress in Malaga, a nutrition system for a lunar base CELSS was presented. A lunar base with a total of eight crew members was envisaged. In this paper, four species of plants--rice, soybean, lettuce and strawberry--were introduced to the system. These plants were sufficient to satisfy fundamental nutritional needs of the crew members. The supply of nutrition from plants and the human nutritional requirements could almost be balanced. Our study revealed that the necessary plant cultivation area per crew member would be nearly 40 m3 in the lunar base. The sources of nutrition considered in the study were energy, sugar, fat, amino acids, inorganic salt and vitamins; however, calcium, vitamin B2, vitamin A and sodium were found to be lacking. Therefore, a subsystem to supply these elements is of considerable value. In this paper, we report on a study for breeding snails and utilizing meat as food. Nutrients supplied from snails are shown to compensate for the above mentioned lacking elements. We evaluate the snail breeder and the associated food supply system as a subsystem of closed ecological life support system.     

Spatial orientation during locomotion [correction of locomation] following space flight

To investigate changes in spatial orientation ability and walking performance following space flight, 7 astronaut subjects were asked pre- and post-flight to perform a goal directed locomotion paradigm which consisted of walking a triangular path with and without vision. This new paradigm, involving inputs from different sensory systems, allows quantification of several critical parameters, like orientation performance, walking velocities and postural stability, in a natural walking task. The paper presented here mainly focusses on spatial orientation performance quantified by the errors in walking the previously seen path without vision. Errors in length and reaching the corners did not change significantly from pre- to post-flight, while absolute angular errors slightly increased post-flight. The significant decrease in walking velocity and a change in head-trunk coordination while walking around the corners of the path observed post-flight may suggest that during re-adaptation to gravity the mechanisms which are necessary to perform the task have to be re-accomplished.     

Learning from living in space

The current state of space life sciences knowledge and research is described. Findings about the health of astronauts in space are reviewed and a plea is made by some former astronauts to increase the amount of research being conducted. Longitudinal studies of the long term effects of space travel, especially radiation exposure, are being conducted and have yet to show any ill effects. Current research focuses are discussed, including Neurolab, an upcoming shuttle mission devoted to neurological and vestibular research. Experiment and spacecraft hardware is discussed, as are future directions in research. Partnership with Russian space life sciences investigators is also underway.     

采用斜坡凹槽稳焰器强化H2超燃的数值研究

用迎风TVD格式求解三维多组分体系的全N－S方程，化学反应源项采用点隐处理，H2和空气的超燃模型用11组分、23步基地反应模型描述，得到了凹槽燃烧室不同截面组元、当量比、压力、温度等值线和速度场的分.布计算结果表明：凹槽稳焰器的回流区可提供高温自由基，有助于超燃点火；火焰在凹槽内和凹槽后斜坡的尾迹中驻定。结果对定量认识凹槽H2超燃流场、设计高效率和优化结构的一体化凹槽有着重要意义。     

Finite-dimensional filters with nonlinear drift. V: solution to Kolmogorov equation arising from linear filtering with non-Gaussian initial condition

Despite its usefulness, the Kalman-Bucy filter is not perfect. One of its weaknesses is that it needs a Gaussian assumption on the initial data. Recently Yau and Yau introduced a new direct method to solve the estimation problem for linear filtering with non-Gaussian initial data. They factored the problem into two parts: (1) the on-line solution of a finite system of ordinary differential equations (ODEs), and (2) the off-line calculation of the Kolmogorov equation. Here we derive an explicit closed-form solution of the Kolmogorov equation. We also give some properties and conduct a numerical study of the solution.     

Phase Space Density Analysis of Energy Transport in the Earth’s Magnetotail

Two energetic events in the Earth’s magnetotail detected by Geotail are examined with detailed analysis of three-dimensional velocity phase space density. It is found that the occurrence of multiple ion components is high during these dynamic episodes. Different populations evolve independently of each other, suggesting particles from multiple activity sites contributing to the observed phase space density. The transport properties with consideration of multiple components are evaluated, with the result showing significant differences from those based on a single fluid approach. This comparison indicates that precise evaluation of the energy and magnetic flux transport of energetic events in the magnetotail requires resolving individual populations in the phase space density.