An examination of the carbon isotope effects associated with amino acid biosynthesis

Stable carbon isotope ratios (delta(13)C) were determined for alanine, proline, phenylalanine, valine, leucine, isoleucine, aspartate (aspartic acid and asparagine), glutamate (glutamic acid and glutamine), lysine, serine, glycine, and threonine from metabolically diverse microorganisms. The microorganisms examined included fermenting bacteria, organotrophic, chemolithotrophic, phototrophic, methylotrophic, methanogenic, acetogenic, acetotrophic, and naturally occurring cryptoendolithic communities from the Dry Valleys of Antarctica. Here we demonstrated that reactions involved in amino acid biosynthesis can be used to distinguish amino acids formed by life from those formed by nonbiological processes. The unique patterns of delta(13)C imprinted by life on amino acids produced a biological bias. We also showed that, by applying discriminant function analysis to the delta(13)C value of a pool of amino acids formed by biological activity, it was possible to identify key aspects of intermediary carbon metabolism in the microbial world. In fact, microorganisms examined in this study could be placed within one of three metabolic groups: (1) heterotrophs that grow by oxidizing compounds containing three or more carbon-to-carbon bonds (fermenters and organotrophs), (2) autotrophs that grow by taking up carbon dioxide (chemolitotrophs and phototrophs), and (3) acetoclastic microbes that grow by assimilation of formaldehyde or acetate (methylotrophs, methanogens, acetogens, and acetotrophs). Furthermore, we demonstrated that cryptoendolithic communities from Antarctica grouped most closely with the autotrophs, which indicates that the dominant metabolic pathways in these communities are likely those utilized for CO(2 )fixation. We propose that this technique can be used to determine the dominant metabolic types in a community and reveal the overall flow of carbon in a complex ecosystem.     

Canadian advanced life support capacities and future directions

Canada began research on space-relevant biological life support systems in the early 1990s. Since that time Canadian capabilities have grown tremendously, placing Canada among the emerging leaders in biological life support systems. The rapid growth of Canadian expertise has been the result of several factors including a large and technically sophisticated greenhouse sector which successfully operates under challenging climatic conditions, well planned technology transfer strategies between the academic and industrial sectors, and a strong emphasis on international research collaborations. Recent activities such as Canada’s contribution of the Higher Plant Compartment of the European Space Agency’s MELiSSA Pilot Plant and the remote operation of the Arthur Clarke Mars Greenhouse in the Canadian High Arctic continue to demonstrate Canadian capabilities with direct applicability to advanced life support systems. There is also a significant latent potential within Canadian institutions and organizations with respect to directly applicable advanced life support technologies. These directly applicable research interests include such areas as horticultural management strategies (for candidate crops), growth media, food processing, water management, atmosphere management, energy management, waste management, imaging, environment sensors, thermal control, lighting systems, robotics, command and data handling, communications systems, structures, in-situ resource utilization, space analogues and mission operations. With this background and in collaboration with the Canadian aerospace industry sector, a roadmap for future life support contributions is presented here. This roadmap targets an objective of at least 50% food closure by 2050 (providing greater closure in oxygen, water recycling and carbon dioxide uptake). The Canadian advanced life support community has chosen to focus on lunar surface infrastructure and not low Earth orbit or transit systems (i.e. microgravity applications). To advance the technical readiness for the proposed lunar missions, including a lunar plant growth lander, lunar “salad machine” (i.e. small scale plant production unit) and a full scale lunar plant production system, a suite of terrestrial developments and analogue systems are proposed. As has been successfully demonstrated by past Canadian advanced life support activities, terrestrial technology transfer and the development of highly qualified personnel will serve as key outputs for Canadian advanced life support system research programs. This approach is designed to serve the Canadian greenhouse industry by developing compliance measures for mitigating environmental impact, reducing labour and energy costs as well as improving Canadian food security, safety and benefit northern/remote communities.     

Glycine identification in natural jarosites using laser desorption Fourier transform mass spectrometry: implications for the search for life on Mars

The jarosite group minerals have received increasing attention since the discovery of jarosite on the martian surface by the Mars Exploration Rover Opportunity. Given that jarosite can incorporate foreign ions within its structure, we have investigated the use of jarosite as an indicator of aqueous and biological processes on Earth and Mars. The use of laser desorption Fourier transform mass spectrometry has revealed the presence of organic matter in several jarosite samples from various locations worldwide. One of the ions from the natural jarosites has been attributed to glycine because it was systematically observed in combinations of glycine with synthetic ammonium and potassium jarosites, Na(2)SO(4) and K(2)SO(4). The ability to observe these organic signatures in jarosite samples with an in situ instrumental technique, such as the one employed in this study, furthers the goals of planetary geologists to determine whether signs of life (e.g., the presence of biomolecules or biomolecule precursors) can be detected in the rock record of terrestrial and extraterrestrial samples.     

Lunar Reconnaissance Orbiter Overview: The Instrument Suite and Mission

NASA’s Lunar Precursor Robotic Program (LPRP), formulated in response to the President’s Vision for Space Exploration, will execute a series of robotic missions that will pave the way for eventual permanent human presence on the Moon. The Lunar Reconnaissance Orbiter (LRO) is first in this series of LPRP missions, and plans to launch in October of 2008 for at least one year of operation. LRO will employ six individual instruments to produce accurate maps and high-resolution images of future landing sites, to assess potential lunar resources, and to characterize the radiation environment. LRO will also test the feasibility of one advanced technology demonstration package. The LRO payload includes: Lunar Orbiter Laser Altimeter (LOLA) which will determine the global topography of the lunar surface at high resolution, measure landing site slopes, surface roughness, and search for possible polar surface ice in shadowed regions, Lunar Reconnaissance Orbiter Camera (LROC) which will acquire targeted narrow angle images of the lunar surface capable of resolving meter-scale features to support landing site selection, as well as wide-angle images to characterize polar illumination conditions and to identify potential resources, Lunar Exploration Neutron Detector (LEND) which will map the flux of neutrons from the lunar surface to search for evidence of water ice, and will provide space radiation environment measurements that may be useful for future human exploration, Diviner Lunar Radiometer Experiment (DLRE) which will chart the temperature of the entire lunar surface at approximately 300 meter horizontal resolution to identify cold-traps and potential ice deposits, Lyman-Alpha Mapping Project (LAMP) which will map the entire lunar surface in the far ultraviolet. LAMP will search for surface ice and frost in the polar regions and provide images of permanently shadowed regions illuminated only by starlight. Cosmic Ray Telescope for the Effects of Radiation (CRaTER), which will investigate the effect of galactic cosmic rays on tissue-equivalent plastics as a constraint on models of biological response to background space radiation. The technology demonstration is an advanced radar (mini-RF) that will demonstrate X- and S-band radar imaging and interferometry using light weight synthetic aperture radar. This paper will give an introduction to each of these instruments and an overview of their objectives.     

The Swift Ultra-Violet/Optical Telescope

The Ultra-Violet/Optical Telescope (UVOT) is one of three instruments flying aboard the Swift Gamma-ray Observatory. It is designed to capture the early (∼1 min) UV and optical photons from the afterglow of gamma-ray bursts in the 170–600 nm band as well as long term observations of these afterglows. This is accomplished through the use of UV and optical broadband filters and grisms. The UVOT has a modified Ritchey–Chrétien design with micro-channel plate intensified charged-coupled device detectors that record the arrival time of individual photons and provide sub-arcsecond positioning of sources. We discuss some of the science to be pursued by the UVOT and the overall design of the instrument.     

Long-term manipulations of intact microbial mat communities in a greenhouse collaboratory: simulating earth's present and past field environments

Photosynthetic microbial mat communities were obtained from marine hypersaline saltern ponds, maintained in a greenhouse facility, and examined for the effects of salinity variations. Because these microbial mats are considered to be useful analogs of ancient marine communities, they offer insights about evolutionary events during the >3 billion year time interval wherein mats co-evolved with Earth's lithosphere and atmosphere. Although photosynthetic mats can be highly dynamic and exhibit extremely high activity, the mats in the present study have been maintained for >1 year with relatively minor changes. The major groups of microorganisms, as assayed using microscopic, genetic, and biomarker methodologies, are essentially the same as those in the original field samples. Field and greenhouse mats were similar with respect to rates of exchange of oxygen and dissolved inorganic carbon across the mat-water interface, both during the day and at night. Field and greenhouse mats exhibited similar rates of efflux of methane and hydrogen. Manipulations of salinity in the water overlying the mats produced changes in the community that strongly resemble those observed in the field. A collaboratory testbed and an array of automated features are being developed to support remote scientific experimentation with the assistance of intelligent software agents. This facility will permit teams of investigators the opportunity to explore ancient environmental conditions that are rare or absent today but that might have influenced the early evolution of these photosynthetic ecosystems.     

Structure of the migrating diurnal tide in the Whole Atmosphere Community Climate Model (WACCM)

As part of an ongoing effort to understand the migrating diurnal tide generated by the NCAR Whole Atmosphere Community Climate Model, version 3 (WACCM3), we compare the WACCM3 migrating diurnal tide in the horizontal wind and temperature fields to similar results from the Global Scale Wave Model (GSWM). The WACCM3 diurnal tidal wind fields are also compared to tropical radar measurements at Kauai (22°N, 200.2°E) and Rarotonga (21.3°S, 199.7°E). The large-scale features of the WACCM3 results, such as the global spatial structure and the semiannual amplitude variation are in general agreement with past tidal studies; however, several differences do exist. WACCM3 exhibits a much higher degree of hemispheric asymmetry, lower overall amplitudes around the equinoxes, and peaks which are more confined in latitude when compared with the GSWM. Factors which may contribute to such differences between WACCM3 and GSWM are the solar heating profiles from ozone and water vapor, dissipation, and the zonal mean zonal winds. We find that the internally generated heating in WACCM3 and eddy dissipation values are both smaller than the values specified in the GSWM; the eddy dissipation fields and zonal mean zonal winds of the two models also display measurable differences in spatial structure. Comparisons with radar data show several differences in spatial and seasonal structure. In particular, the diurnal tide zonal winds in WACCM3 above Kauai are considerably larger in amplitude than those observed in the radar data, due to contributions from nonmigrating tidal components including wave numbers eastward 1 through 3, westward 2, and stationary components, which interfere constructively with the migrating component around equinox in WACCM3.     

Challenges to US space sustainability

With space now crucial to such a wide range of activities on Earth, the USA must ensure the sustainability of its efforts, a task that involves technological feasibility and political will. Near-term challenges include US human access to space and the Shuttle transition, funding NASA sufficiently in a time of recession, and rebuilding the country's space industrial base. Longer-term challenges will be better protecting the space environment (including the electromagnetic spectrum) from overcrowding and the effects of space weather and NEOs, and defining responsibilities for distributing climate change data and recognition of property rights for the commercial development of in-space resources. As an aid to dealing with these challenges the USA must ask itself whether there is a human future in space and seek to answer the question in the course of human and robotic exploration beyond Earth.     

InSight Mars Lander Robotics Instrument Deployment System

The InSight Mars Lander is equipped with an Instrument Deployment System (IDS) and science payload with accompanying auxiliary peripherals mounted on the Lander. The InSight science payload includes a seismometer (SEIS) and Wind and Thermal Shield (WTS), heat flow probe (Heat Flow and Physical Properties Package, HP³) and a precision tracking system (RISE) to measure the size and state of the core, mantle and crust of Mars. The InSight flight system is a close copy of the Mars Phoenix Lander and comprises a Lander, cruise stage, heatshield and backshell. The IDS comprises an Instrument Deployment Arm (IDA), scoop, five finger “claw” grapple, motor controller, arm-mounted Instrument Deployment Camera (IDC), lander-mounted Instrument Context Camera (ICC), and control software. IDS is responsible for the first precision robotic instrument placement and release of SEIS and HP³ on a planetary surface that will enable scientists to perform the first comprehensive surface-based geophysical investigation of Mars’ interior structure. This paper describes the design and operations of the Instrument Deployment Systems (IDS), a critical subsystem of the InSight Mars Lander necessary to achieve the primary scientific goals of the mission including robotic arm geology and physical properties (soil mechanics) investigations at the Landing site. In addition, we present test results of flight IDS Verification and Validation activities including thermal characterization and InSight 2017 Assembly, Test, and Launch Operations (ATLO), Deployment Scenario Test at Lockheed Martin, Denver, where all the flight payloads were successfully deployed with a balloon gravity offload fixture to compensate for Mars to Earth gravity.