Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.

The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.     

New insights into Titan's organic chemistry in the gas and aerosol phases.

Titan, the largest satellite of Saturn, with a dense atmosphere very rich in organics, and many couplings in the various parts of its "geofluid", is a reference for studying prebiotic chemistry on a planetary scale. New data have been obtained from experiments simulating this organic chemistry (gas and aerosol phases), within the right ranges of temperature and a careful avoiding of any chemical contamination. They show a very good agreement with the observational data, demonstrating for the first time the formation of all the organic species already detected in Titan atmosphere including, at last, C4N2, together with many other species not yet detected in Titan. This strongly suggests the presence of more complex organics in Titan's atmosphere and surface, including high molecular weight polyynes and cyanopolyynes. The NASA-ESA Cassini-Huygens mission has been successfully launched in October 1997. The Cassini spacecraft will reach the Saturn system in 2004 and become an orbiter around Saturn, while the Huygens probe will penetrate into Titan's atmosphere. In situ measurements, in particular from Huygens GC-MS and ACP instruments, will provide a detailed analysis of the organics present in the air, aerosols, and surface. This very ambitious mission should yield much information of crucial importance for our knowledge of the complexity of Titan's chemistry, and, more generally for the field of exobiology.     

The Galileo Dust Detector

The Galileo Dust Detector is intended to provide direct observations of dust grains with masses between 10^-19 and 10^-9 kg in interplanetary space and in the Jovian system, to investigate their physical and dynamical properties as functions of the distances to the Sun, to Jupiter and to its satellites, to study its interaction with the Galilean satellites and the Jovian magnetosphere. Surface phenomena of the satellites (like albedo variations), which might be effects of meteoroid impacts will be compared with the dust environment. Electric charges of particulate matter in the magnetosphere and its consequences will be studied; e.g., the effects of the magnetic field on the trajectories of dust particles and fragmentation of particles due to electrostatic disruption. The investigation is performed with an instrument that measures the mass, speed, flight direction and electric charge of individual dust particles. It is a multicoincidence detector with a mass sensitivity 10⁶ times higher than that of previous in-situ experiments which measured dust in the outer solar system. The instrument weighs 4.2 kg, consumes 2.4 W, and has a normal data transmission rate of 24 bits s^-1 in nominal spacecraft tracking mode. On December 29, 1989 the instrument was switched-on. After the instrument had been configured to flight conditions cruise science data collection started immediately. In the period to May 18, 1990 at least 168 dust impacts have been recorded. For 81 of these dust grains masses and impact speeds have been determined. First flux values are given.     

Single-phase power distribution system power flow and faultanalysis

Alternative methods for power flow and fault analysis of single-phase distribution systems are presented. The algorithms for both power flow and fault analysis utilize a generalized approach to network modeling. The generalized admittance matrix, formed using elements of linear graph theory, is an accurate network model for all possible single-phase network configurations. Unlike the standard nodal admittance matrix formulation algorithms, the generalized approach uses generalized component models for the transmission line and transformer. The standard assumption of a common node voltage reference point is not required to construct the generalized admittance matrix. Therefore, truly accurate simulation results can be obtained for networks that cannot be modeled using traditional techniques     

Radial and meridional trends in solar wind thermal electron temperature and anisotropy: Ulysses

Ulysses plasma measurement from 1.15 to 5.31 AU and from S6.4° to S48.3° solar latitude are used to assess the trends in the solar wind thermal electron temperature and anisotropy. Improved spacecraft potential corrections and data products have been incorporated. The radial temperature gradient is steeper than in previous determinations, but flatter than adiabatic. When normalized to 1 AU, temperature decrease with increasing latitude. Little change in the average thermal anisotropy has been seen during the mission.     

Interacting multiple model tracking with target amplitude feature

A recursive tracking algorithm is presented which uses the strength of target returns to improve track formation performance and track maintenance through target maneuvers in a cluttered environment. This technique combines the interacting multiple model (IMM) approach with a generalized probabilistic data association (PDA), which uses the measured return amplitude in conjunction with probabilistic models for the target and clutter returns. Key tracking decisions can be made automatically by assessing the probabilities of target models to provide rapid and accurate decisions for both true track acceptance and false track dismissal in track formation. It also provides the ability to accurately continue tracking through coordinated turn target maneuvers     

On phased-array radar tracking and parameter control

Based on a simple model of a ground-based phased-array radar used for a multiple-target surveillance task, beam scheduling, positioning, and radar parameters like signal-to-noise ratio, track sharpness, and detection threshold have been optimized with respect to the radar/computer load induced by tracking. From this the minimum energy necessary for track maintenance during surveillance can be derived     

Solar wind corotating stream interaction regions out of the ecliptic plane: Ulysses

Ulysses plasma observations reveal that the forward shocks that commonly bound the leading edges of corotating interaction regions (CIRs) beyond 2 AU from the Sun at low heliographic latitudes nearly disappeared at a latitude of S26°. On the other hand, the reverse shocks that commonly bound the trailing edges of the CIRs were observed regularly up to S41.5°, but became weaker with increasing latitude. Only three CIR shocks have been observed poleward of S41.5°; all of these were weak reverse shocks. The above effects are a result of the forward waves propagating to lower heliographic latitudes and the reverse waves to higher latitudes with increasing heliocentric distance. These observational results are in excellent agreement with the predictions of a global model of solar wind flows that originate in a simple tilted-dipole geometry back at the Sun.     

Ulysses out-of-ecliptic observations of “27-day” variations in high energy cosmic ray intensity

We report the discovery that for latitudes above 40°S, the observed recurring modulation of cosmic rays and anomalous nuclei occurs without the detection byUlysses of the solar wind velocity and magnetic field recurring enhancements that have, heretofore at lower latitudes, defined corotating interaction regions—i.e., the mechanism producing the recurring intensity variations >40°S appears to be located beyond the radial range ofUlysses.     

Communications and radio determination system using twogeostationary satellites. I. System and experiments

We developed two types of hybrid terminals that can provide both satellite communication and position determination services in one system. One terminal uses the single channel per carrier (SCPC) technique and the other uses the spread spectrum (SS) technique. To evaluate the performance of the two systems, we carried out experiments in Japan and in the Pacific Ocean using two geostationary satellites, ETS-V (150°E) and Inmarsat (180°E). The ranging accuracy between the mobile terminals and the base station via the satellites was found to be about 200 m using the SCPC system and about 10 m using the SS system. The measured positioning accuracy was about 1 km in the SCPC system and about 600 m in the SS system when experiments were carried out near Japan. The experimental results show that the positioning errors were mainly caused by the orbital determination errors of the two satellites. Presented here are the configurations and features of the SCPC and SS terminals, the experimental system, and the experimental results