Choroidal responses in microgravity. (SLS-1, SLS-2 and hindlimb-suspension experiments)

Fluid and electrolyte shifts occuring during human spaceflight have been reported and investigated at the level of blood, cardio-vascular and renal responses. Very few data were available concerning the cerebral fluid and electrolyte adaptation to microgravity, even in animal models. It is the reason why we developed several studies focused on the effects of spaceflight (SLS-1 and SLS-2 programs, carried on NASA STS 40 and 56 missions, which were 9- and 14-day flights, respectively), on structural and functional features of choroid plexuses, organs which secrete 70–90 % of cerebrospinal fluid (CSF) and which are involved in brain homeostasis. Rats flown aboard space shuttles were sacrificed either in space (SLS-2 experiment, on flight day 13) or 4–8 hours after landing (SLS-1 and SLS-2 experiments). Quantitative autoradiography performed by microdensitometry and image analysis, showed that lateral and third ventricle choroid plexuses from rats flown for SLS-1 experiment demonstrated an increased number (about x 2) of binding sites to natriuretic peptides (which are known to be involved in mechanisms regulating CSF production). Using electron microscopy and immunocytochemistry, we studied the cellular response of choroid plexuses, which produce cerebrospinal fluid (CSF) in brain lateral, third and fourth ventricles. We demonstrated that spaceflight (SLS-2 experiment, inflight samples) induces changes in the choroidal cell structure (apical microvilli, kinocilia organization, vesicle accumulation) and protein distribution or expression (carbonic anhydrase II, water channels,…). These observations suggested a loss of choroidal cell polarity and a decrease in CSF secretion. Hindlimb-suspended rats displayed similar choroidal changes. All together, these results support the hypothesis of a modified CSF production in rats during long-term (9, 13 or 14 days) adaptations to microgravity.     

Effect of fluid and salt supplements in preventing the development of "osteopenia" in hypokinetic rats

It has been suggested that a daily intake of fluid and salt supplements may be used to prevent bone demineralization in human subjects after prolonged exposure to hypokinesia (diminished muscular activity). Thus, the objective of this investigation was to evaluate the effect of fluid and salt supplementation in the prevention of development of osteoporosis in 64 Wistar rats with an initial body weight of 339-345 g, after exposure to 90 days of hypokinesia. They divided into 4 equal groups: the first group of rats placed under ordinary vivarium conditions and served as vivarium control; the second group were also placed under ordinary vivarium conditions but received daily fluid and salt supplements; the third group were subjected to pure hypokinesia, i.e. without the use of any preventive measures; and the fourth group were submitted to hypokinesia and received daily fluid and salt supplements. For the simulation of the hypokinetic effect the experimental group of rats were kept in small, individual, wooden cages. Through the experimental period the second and fourth group of rats received 8 ml/100 g body wt water and 5 ml 100 g body wt NaCl daily. By the end of the experimental period the animals were decapitated and the spongy matter of tibia and vertebrae of the rats were examined for changes referable to osteoporosis. It was found that the daily intake of fluid and salt supplements caused an increase in the volume density of primary spongiosa of bones. It was concluded that a daily intake of fluid and salt supplements may be used to prevent the development of osteoporosis in rats subjected to prolonged motor activity restriction.     

The effect of small vibrations on Marangoni convection and the free surface of a liquid bridge

The effects of small vibrations on Marangoni convection were investigated experimentally using a liquid bridge of 5 cSt silicone oil with a disk diameter of 7.0 mm, and an aspect ratio close to 0.5. Experiments were performed to determine the critical temperature difference data for no vibration case and with small vibrations applied. The experimental results have shown that the effect of small vibrations on the onset of oscillatory flow is small since the critical temperature difference data for different aspect ratios were not affected by the vibrations. To clarify the surface oscillation phenomena induced by external vibrations, a 3-D numerical simulation model was also developed using a level set algorithm to predict the surface oscillations of isothermal silicone oil bridges. By subjecting the liquid bridge to small vibrations, the surface oscillation characteristics were predicted numerically, and the numerical results compared well with the predictions of an analytical model proposed previously. Furthermore, the effect of small vibrations on the surface vibration amplitude of the liquid bridge is also discussed.     

Precipitating Protons and Their Role in Ionization of the Polar Ionosphere

Spatial distributions of the electron density in the latitude range 60°–90° N were calculated on the basis of a physical model of the E and lower Fregions of the high-latitude ionosphere using statistical models of auroral proton and electron precipitation. It is shown that precipitating protons can play the key role in the ionization of the Eregion in the dusk and midnight sectors of the auroral oval. However, quantitative estimates of the contribution of protons to the ionization depend on the used statistical models of electron precipitation. Comparison of the electron density profiles calculated for two incoherent scatter radars, EISCAT (Tromsö) and ESR (Svalbard), for simultaneous precipitation of electrons and protons and for electron precipitation only show that the influence of protons is the most significant in the dusk sector over the EISCAT radar and in the midnight sector over the ESR radar. The results presented indicate the need to take protons into account when radar data are used to derive precipitating electron spectra.     

The Brazilian scientific microsatellite SACI-1

The National Space Research Institute (INPE) is developing the first Brazilian Scientific Microsatellite (SACI-1) based on the vanguard technology and on the experience acquired through projects developed by Brazilian Space Program. The SACI-1 is a 750km polar orbit satellite. The spacecraft will combine spin stabilization with geomagnetic control and has a total mass of 60 kg. The overall dimensions are 640×470×470 mm. The SACI-1 satellite shall be launched together with CBERS (China-Brazil Earth Resource Satellite). Its platform is being designed for multiple mission applications. The Brazilian Academy of Sciences has selected four scientific payloads that characterize the mission. The scientific experiments are: ORCAS (Solar and Anomalous Cosmic Rays Observation in the Magnetosphere), PLASMEX (Study of Plasma Bubbles), FOTSAT (Airglow Photometer), and MAGNEX (Geomagnetic Experiment).     

Drying as one of the extreme factors for the microflora of the atmosphere

An investigation of the effects of drying and drying-rewetting cycles under vacuum conditions of 10(-2) torr at -30 degrees C on conidia of the atmospheric strain Penicillium chrysogenum from 48-77 km heights, collected by meteorological rockets found that survival and restoration of the initial physiological properties were possible through some very efficient protective cellular mechanisms. Such adaptive possibilities explain the widespread diffusion of microbial cells throughout the biosphere of our planet.     

Phugoid oscillations in optimal reentry trajectories

A major problem with operations of lifting reentry vehicle having an aft center-of-gravity location due to large engine mass at the rear is the required hypersonic trim to fight the desired trajectory. This condition is most severe for lifting maneuvers. As a first step toward analyzing this problem, this paper considers the lift requirement for some basic maneuvers in the plane of a great circle. Considerations are given to optimal lift control for achieving the maximization of either the final altitude, speed or range. For the maximum-range problem, phugoid oscillation along an optimal trajectory is less severe as compared to a glide with maximum lift-to-drag ratio. An explicit formula for the number of oscillations for an entry from orbital speed is proposed.     

"Signal" search for intelligence in the galactic nucleus with the array of the Lowlands

In August, 1981, the Westerbork Synthesis Radio Telescope was used for 4 h to search for narrowband pulsing radio beacons in the direction of the Galactic Center. By using both the spatial discrimination and temporal stability available to an interferometric measurement, weak intermittent signals can be detected even in the face of the strong, naturally caused radiation from this region. A radio beacon within our bandwidth, centered on the 21 cm neutral hydrogen line, would be recognizable if it had a repetition period between 40 sec and 1/2 h. The rms sensitivity to point sources was approximately 50 mJy/cycle, and the detection limit was 500 mJy/cycle. The limit degrades for pulse widths < 0.02s. No repetitive signals were found. For a swept, narrow-band radio beacon constrained to the Galactic Disk (beamwidth = 0.02 rad), our detection limit corresponds to a transmitter power of 10(11) MW at the Galactic Center.