Repair of DNA double-strand breaks and cell killing by charged particles.

It has been suggested that it is not simple double-strand breaks (dsb) but the non-reparable breaks which correlate well with the high biological effectiveness of high LET radiations for cell killing (Kelland et al., 1988; Radford, 1986). We have compared the effects of charged particles on cell death in 3 pairs of cell lines which are normal or defective in the repair of DNA dsbs. For the cell lines SL3-147, M10, and SX10 which are deficient in DNA dsb repair, RBE values were close to unity for cell killing induced by charged particles with linear energy transfer (LET) up to 200 keV/micrometer and were even smaller than unity for the LET region greater than 300 keV/micrometer. The inactivation cross section (ICS) increased with LET for all 3 pairs. The ICS of dsb repair deficient mutants was always larger than that of their parents for all the LET ranges, but with increasing LET the difference in ICS between the mutant and its parent became smaller. Since a small difference in ICS remained at LET of about 300 keV/micrometer, dsb repair may still take place at this high LET, even if its role is apparently small. These results suggest that the DNA repair system does not play a major role in protection against the attack of high LET radiations and that a main muse of cell death is non-reparable dsb which are produced at a higher yield compared with low LET radiations. No correlation was observed between DNA content or nuclear area and ICS.     

Numerical prediction and wind tunnel experiment for a pitching unmanned combat air vehicle

The low-speed flowfield for a generic unmanned combat air vehicle (UCAV) is investigated both experimentally and numerically. A wind tunnel experiment was conducted with the Boeing 1301 UCAV at a variety of angles of attack up to 70 degrees, both statically and with various frequencies of pitch oscillation (0.5, 1.0, and 2.0 Hz). In addition, pitching was performed about three longitudinal locations on the configuration (the nose, 35% MAC, and the tail). Solutions to the unsteady, laminar, compressible Navier–Stokes equations were obtained on an unstructured mesh to match results from the static and dynamic experiments. The computational results are compared with experimental results for both static and pitching cases. Details about the flowfield, including vortex formation and interaction, are shown and discussed, including the non-linear aerodynamic characteristics of the vehicle.     

On the Measurements of D/H in QSO Absorption Systems Closing in on the primordial abundance of deuterium

We present our measurements of the deuterium to hydrogen ratio (D/H) in QSO absorption systems, which give D/H = 3.40 ± 0.25 × 10-5 based on analysis of four independent systems. We discuss the properties of two systems which provide the strongest constraints on D/H. We outline the systematic effects involved in measurements of D/H and introduce a sophisticated method of analysis which properly accounts for these effects.     

Adaptive detection schemes in compound-Gaussian clutter

Radar detection of coherent pulse trains embedded in compound-Gaussian disturbance with partially known statistics is discussed. We first give a thorough derivation of two recently proposed adaptive detection structures. Next, we derive a different detection scheme exploiting the assumption that the clutter is wide-sense stationary. Resorting to the theory of circulant matrices, in fact, we demonstrate that the estimation of the structure of the clutter covariance matrix can be reduced to the estimation of its eigenvalues, which in turn can be (efficiently) done via fast Fourier transform codes. After a thorough performance assessment, mostly carried on via computer simulations, the results show that the newly proposed detector achieves better performance than the two previously introduced adaptive detectors. Moreover, a sensitivity analysis shows that, even though this detector does not strictly guarantee the constant false alarm rate property with respect to the clutter covariance matrix, it is robust, in the sense that its performance is only slightly affected by variations in the clutter temporal correlation     

Empirical modeling of the total electron content in the ionosphere

A method of modeling the total electron content (TEC) based on the semi-empirical ionospheric model developed in Irkutsk State University is suggested. Comparison with the Klobuchar model has shown that the proposed method provides a more accurate presentation of TEC. A conclusion is drawn that the use of this method for compensation of the ionospheric error in single-frequency navigation receivers would lead to a substantial increase in the accuracy of their positioning.     

Solar Models with Non-Standard Chemical Composition

The OPAL monochromatic opacity tables are used to evaluate the impact of a non-standard chemical composition on solar models. A calibrated solar model with consistent diffusion including the effect of radiative forces and ionization on drift velocities is presented. It is shown that surface abundances are predicted to change slightly more than in traditional solar models where these refinements are not included. All elements included in the model settle at similar rates which is reflected in the relative variation in surface abundances ranging from 7.5% for calcium to 8.8% for argon. The structural difference between the consistent model and the traditional model is small, with a maximum effect of 0.3% for the isothermal sound speed at the base of the convection zone. The settling of CNO is only marginally affected. Opacity profiles have also been calculated with varying abundances for volatile elements, for which the abundances are poorly known, and other selected elements. It is shown that if one allows a 10% variation of these elements individually one can expect a peak Rosseland mean opacity variation of 3% for oxygen, a little less 2% for Si and Ne, and around 1% for Mg and S in the radiative zone. Other light metals and volatile elements have no significant impact on the opacity. This revised version was published online in June 2006 with corrections to the Cover Date.     

Maps of hydrogen isotopes at low altitudes in the inner zone from sampex observations

The PET instrument aboard the SAMPEX satellite has provided us with long-term intra-calibrated observations of geomagnetically trapped protons and deuterons in the inner zone, suitable for use in constraining the low-altitude portions of radiation belt models being developed as successors to AP-8. These observations have been summarized elsewhere (Looper et al., 1996). Here we report a detection of geomagnetically-trapped tritium at energies from 14 to 35 MeV/nuc below L = 1.2, at about 1/8 the flux of deuterium previously reported at that location and at similar energy per nucleon. We also demonstrate the utility of the SAMPEX/PET observations for measuring the east-west anisotropy in the trapped particle flux at low altitudes, which is due to displacement of particle gyrocenters from the position of observation in a region of strong flux gradients. This anisotropy is implicitly ignored in omnidirectional radiation-flux models, but it can be important to mission planners considering how to distribute shielding over the surface of oriented spacecraft in low Earth orbit.     

Effect of track structure and radioprotectors on the induction of oncogenic transformation in murine fibroblasts by heavy ions

The oncogenic potential of high-energy ⁵⁶Fe particles (1 GeV/nucleon) accelerated with the Alternating Gradient Synchrotron at the Brookhaven National Laboratory was examined utilizing the mouse C3H 10T1/2 cell model. The dose-averaged LET for high-energy ⁵⁶Fe is estimated to be 143 keV/μm with the exposure conditions used in this study. For ⁵⁶Fe ions, the maximum relative biological effectiveness (RBE_max) values for cell survival and oncogenic transformation were 7.71 and 16.5 respectively. Compared to 150 keV/μm ⁴He nuclei, high-energy ⁵⁶Fe nuclei were significantly less effective in cell killing and oncogenic induction. The prostaglandin E₁ analog misoprostol, an effective oncoprotector of C3H 10T1/2 cells exposed to X rays, was evaluated for its potential as a radioprotector of oncogenic transformation with high-energy ⁵⁶Fe. Exposure of cells to misoprostol did not alter ⁵⁶Fe cytotoxicity or the rate of ⁵⁶Fe-induced oncogenic transformation.     

Tissue responses to low protracted doses of high LET radiations or photons: early and late damage relevant to radio-protective countermeasures.

Early and late murine tissue responses to single or fractionated low doses of heavy charged particles, fission-spectrum neutrons or gamma rays are considered. Damage to the hematopoietic system is emphasized, but results on acute lethality, host response to challenge with transplanted leukemia cells and life-shortening are presented. Low dose rates per fraction were used in some neutron experiments. Split-dose lethality studies (LD 50/30) with fission neutrons indicated greater accumulation of injury during a 9 fraction course (over 17 days) than was the case for gamma-radiation. When total doses of 96 or 247 cGy of neutrons or gamma rays were given as a single dose or in 9 fractions, a significant sparing effect on femur CFU-S depression was observed for both radiation qualities during the first 11 days, but there was not an earlier return to normal with dose fractionation. During the 9 fraction sequence, a significant sparing effect of low dose rate on CFU-S depression was observed in both neutron and gamma-irradiated mice. CFU-S content at the end of the fractionation sequence did not correlate with measured LD 50/30. Sustained depression of femur and spleen CFU-S and a significant thrombocytopenia were observed when a total neutron dose of 240 cGy was given in 72 fractions over 24 weeks at low dose rates. The temporal aspects of CFU-S repopulation were different after a single versus fractionated neutron doses. The sustained reduction in the size of the CFU-S population was accompanied by an increase in the fraction in DNA synthesis. The proliferation characteristics and effects of age were different for radial CFU-S population closely associated with bone, compared with the axial population that can be readily aspirated from the femur. In aged irradiated animals, the CFU-S proliferation/redistribution response to typhoid vaccine showed both an age and radiation effect. After high single doses of neutrons or gamma rays, a significant age- and radiation-related deficiency in host defense mechanisms was detected by a shorter mean survival time following challenge with transplantable leukemia cells. Comparison of dose-response curves for life shortening after irradiation with fission-spectrum neutrons or high energy silicon particles indicated high initial slopes for both radiation qualities at low doses, but for higher doses of silicon, the effect per Gy decreased to a value similar to that for gamma rays. The two component life-shortening curve for silicon particles has implications for the potential efficacy of radioprotectants. Recent studies on protection against early and late effects by aminothiols, prostaglandins, and other compounds are discussed.     

Dynamics of a Solenoidal-Torqued Gyro-Stabilized Seeker Assembly for Guidance and Tracking

Several low-cost gyro-stabilized, bang-bang seeker assemblies for missile guidance and tracking applications have used similar physical structures. A magnetic dipole is an integral part of the gyro rotor assembly. The nominal spin axis of the gyro is colinear with the axis of a cylinder about which is wound a helix of wire. By properly modulating the current that is passed through that helix, the precession torque acting upon the gyroscope is controlled. One rather generalized model of such a seeker assembly is modeled, the equations of motion are derived, and the dominant error sources are identified and parametrically evaluated. Because this is such a commonly encountered structure, yet no such analysis has been published, to the author's knowledge, it is hoped that this is a useful contribution to the literature.