Distribution of calmodulin in corn seedlings: Immunocytochemical localization in coleoptiles and root apices

Immunofluorescence techniques have been used to study the distribution of calmodulin in several tissues in etiolated corn ( , var. Bear Hybrid) seedlings. Uniform staining was seen in the background cytoplasm of most cell types. Cell walls and vacuoles were not stained. In coleoptile mesophyll cells the nucleoplasm of most nuclei was stained as was the stroma of most amyloplasts. The lumen border of mature tracheary elements in coleoptiles also stained. In the rootcap the most intensely stained regions were the cytoplasms of columella cells and of the outermost cells enmeshed in the layer of secreted slime. Nuclei in the rootcap cells did not stain distinctly, but those in all cell types of the root meristem did. Also in the root meristem, the cytoplasm of metaxylem elements stained brightly. These results are compared and contrasted with previous data on the localization of calmodulin in pea root apices and epicotyls and discussed in relation to current hypotheses on mechanisms of gravitropism.     

X-ray and gamma-ray observations of a white-light flare

HEAO-1 observed hard radiations (X- and gamma-rays) from a major solar flare on 11 July 1978. The observations showed gamma-ray line and continuum emission extending to the highest energy observed. The lines are identified with the 2.2 MeV line of deuterium formation and the 4.4 MeV line of inelastic scattering on ¹²C, both previously observed in the flares of August 1972 [1]. The 11 July flare was identified as a white-light flare by observations at Debrecen [2]. It thus provides the first opportunity for a detailed examination of white-light flare theories that depend upon proton heating of the photosphere. The line strength over a four-minute integration at 2.2 MeV was 1.00 ± 0.29 ph(cm² sec)⁻¹, and the gamma-ray emission (excluding the 2.2 MeV line which was appreciably delayed) lagged by less than 20 sec approximately after the hard X-ray and microwave fluxes. We conclude that the “second-stage” acceleration of high-energy solar particles must commence promptly after the impulsive phase.     

Aqueous corrosion of phosphide minerals from iron meteorites: a highly reactive source of prebiotic phosphorus on the surface of the early Earth

We present the results of an experimental study of aqueous corrosion of Fe-phosphide under conditions relevant to the early Earth. The results strongly suggest that iron meteorites were an important source of reactive phosphorus (P), a requirement for the formation of P-based life. We further demonstrate that iron meteorites were an abundant source of phosphide minerals early in Earth history. Phosphide corrosion was studied in five different solutions: deionized water, deionized water buffered with sodium bicarbonate, deionized water with dissolved magnesium and calcium chlorides, deionized water containing ethanol and acetic acid, and deionized water containing the chlorides, ethanol, and acetic acid. Experiments were performed in the presence of both air and pure Ar gas to evaluate the effect of atmospheric chemistry. Phosphide corrosion in deionized water results in a metastable mixture of mixed-valence, P-bearing ions including pyrophosphate and triphosphate, key components for metabolism in modern life. In a pH-buffered solution of NaHCO(3), the condensed and reduced species diphosphonate is an abundant corrosion product. Corrosion in ethanol- and acetic acid-containing solutions yields additional P-bearing organic molecules, including acetyl phosphonate and a cyclic triphosphorus molecule. Phosphonate is a major corrosion product of all experiments and is the only P-bearing molecule that persists in solutions with high concentrations of magnesium and calcium chlorides, which suggests that phosphonate may have been a primitive oceanic source of P. The stability and reactivity of phosphonate and hypophosphite in solution were investigated to elucidate reaction mechanisms and the role of mineral catalysts on P-solution chemistry. Phosphonate oxidation is rapid in the presence of Fe metal but negligible in the presence of magnetite and in the control sample. The rate of hypophosphite oxidation is independent of reaction substrate.     

Electrostatic Noise Spectrum at the Electron Cyclotron Frequency and Electromagnetic Emission in the Inhomogeneous Plasma of the Topside Ionosphere

Measurements of the wave emission of the topside ionosphere made onboard the APEX satellite using the electric component of the wave field in the 0.1–10 MHz frequency band are presented. At middle latitudes a wave intensity decrease was observed in the broad-band spectrum of the electrostatic noise at the electron cyclotron frequency. It is shown that a break in the spectrum of electrostatic modes at the electron cyclotron frequency (the absence of the plasma eigen-frequencies) may be a cause of the observed effect. The increase of the intensity at the electron cyclotron frequency in the ionospheric trough and at latitudes above the trough region as compared to middle latitudes may be explained by the capture by plasma irregularities of the electromagnetic emission of the auroral electron fluxes.__________Translated from Kosmicheskie Issledovaniya, Vol. 43, No. 3, 2005, pp. 201–208.Original Russian Text Copyright © 2005 by Izhovkina, Prutensky, Pulinets, Kiraga, Klos, Rothkael.     

Effect of variable thermal properties of the solid phase on composite solid propellant combustion

Effects of variable thermal properties (specific heat and thermal conductivity) of the solid phase on the combustion of composite solid propellants are studied analytically. Both steady and unsteady burning are considered. It is shown that the effects of variable thermal properties are small and can be accounted for by choosing proper average values of specific heat and thermal conductivity.     

Plasma irregularities in unstable outer ionosphere according to the Intercosmos-Bulgaria-1300 satellite data

The results of the satellite low-latitude and mid-latitude measurements of the disturbed plasma concentration, electron temperature, and quasi-stable electric field at heights of ～900 km after sunset are discussed. It is shown that the sharp fronts of changes in the electron temperature and plasma density observed in the experiment onboard the Intercosmos-Bulgaria-1300 satellite in the low-latitude (and equatorial) outer ionosphere can be related to damping of the oscillations of plasma electrons at local decreases of the plasma density (plasma “pits”) and formation of the vortex plasma structures at density and temperature gradients, which promotes conservation of ionosphere irregularities and makes the fronts of concentration variations steeper. Nonmonotonic variations in the plasma conductivity for the ionosphere currents in unstable plasma can be a cause of observed nonmonotonic disturbances of the vertical component of the “constant” electric field.     

Stability of resonance rotation of a satellite with respect to its center of mass in the orbit plane

The stability of resonance oscillations and rotations of a satellite in the plane of its orbit in the case when the difference of the moments of inertia with respect to the principal axes lying in the orbit plane is small is determined at a given rotation number m by the sign of function Φ_m(e), introduced by F.L. Chernous’ko in 1963. In this paper, convenient analytical representations of functions Φ_m(e) are described in the form of integrals and series of Bessel functions regular at e → 1^?. Values of Φ_m(1) are calculated in explicit form. A theorem about the double asymptotic form of functions Φ_m(e) at m → ∞ and e → 1^? is proved by the saddlepoint method.     

Numerical simulation of effects of the August 11, 1999 solar eclipse in the outer ionosphere

The results of a numerical simulation of such parameters of the topside ionosphere as concentration N _e and temperature T _e of electrons, and concentration n(H⁺) and fluxes along the magnetic field lines Φ(H⁺) of H⁺ ions at an altitude of ～2000 km for the conditions of the August 11, 1999 solar eclipse are presented. The calculations were performed using the Global Self-consistent Model of the Thermosphere, Ionosphere, and Protonosphere of the Earth (GSM TIP). It is shown that during the eclipse, in addition to a region of decreased values of T _e in the Northern Hemisphere and in the magnetically conjugate region in the Southern Hemisphere, regions of electron heating emerge in both hemispheres. Simultaneously, an extended region of decreased values of N _e comes into existence and moves behind the Moon’s shadow. Regions with decreased (down to ～30%) and enhanced (up to ～50%) concentrations of H⁺ ions are detected in the global distribution of these ions.