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961.
The cytoskeleton is a complex network of fibers that is sensitive to environmental factors including microgravity and altered gravitational forces. Cellular functions such as transport of cell organelles depend on cytoskeletal integrity; regulation of cytoskeletal activity plays a role in cell maintenance, cell division, and apoptosis. Here we report cytoskeletal and mitochondria alterations in cultured human lymphocyte (Jurkat) cells after exposure to spaceflight and in insect cells of Drosophila melanogaster (Schneider S-1) after exposure to conditions created by clinostat rotation. Jurkat cells were flown on the space shuttle in Biorack cassettes while Schneider S-1 cells were exposed to altered gravity forces as produced by clinostat rotation. The effects of both treatments were similar in the different cell types. Fifty percent of cells displayed effects on the microtubule network in both cell lines. Under these experimental conditions mitochondria clustering and morphological alterations of mitochondrial cristae was observed to various degrees after 4 and 48 hours of culture. Jurkat cells underwent cell divisions during exposure to spaceflight but a large number of apoptotic cells was also observed. Similar results were obtained in Schneider S-1 cells cultured under clinostat rotation. Both cell lines displayed mitochondria abnormalities and mitochondria clustering toward one side of the cells which is interpreted to be the result of microtubule disruption and failure of mitochondria transport along microtubules. The number of mitochondria was increased in cells exposed to altered gravity while cristae morphology was severely affected indicating altered mitochondria function. These results show that spaceflight as well as altered gravity produced by clinostat rotation affects microtubule and mitochondria organization and results in increases in apoptosis. Grant numbers: NAG 10-0224, NAG2-985.  相似文献   
962.
High resolution Hα images and magnetograms (0.2 arc s) of an active region were obtained in alternating time series at 42 s cadences using the Swedish 1-m Solar Telescope on 2004 August 21. The Hα filtergrams reveal an active region filament and surges consisting of thread-like structures which have widths similar to the widths of chromospheric fibrils, both recorded down to the resolution limit in the best images. All observed structures in the active region appear highly dynamic. Fibrils show counterstreaming strongly resembling the counterstreaming threads in filaments.  相似文献   
963.
We investigate the generation of ballooning perturbations in the inner magnetosphere of the Earth in the dipole model of the geomagnetic field taking into account ionospheric boundary conditions. The ionosphere is considered as a thin layer with finite conductivity. The eigenmode spectrum is discrete and consists of Alfvén, slow magnetosonic, flute and incompressible modes. Their interaction depends on ionospheric conductivity. The decay rate is small in noon and night sectors and large in dawn and dusk sectors. The lowest stability threshold α/γ ≈ 4.25 is determined by flute modes.  相似文献   
964.
The specifications are presented for an imperfect automatic test system (ATS) (test frequency distribution, reliability, false alarm rate, nondetection rate) in order to account for the availability, readiness, mean time between unscheduled repairs (MTBUR), reliability, and maintenance of the system subject to monitoring and test. A time-dependent Markov model is presented, and applied in three cases, with examples of numerical results provided for preventive maintenance decisions, design of an automatic test system, buffer testing in computers, and data communications.  相似文献   
965.
In today's National Airspace System (NAS), when an application requires information from another application, a custom application-to-application interface is built. This results in an increasingly complex system, where applications are tightly coupled and expensive to develop and maintain. System-Wide Information Management (SWIM) addresses these shortfalls by implementing a shared infrastructure for managing NAS information. SWIM is based on a service-oriented architecture, a fast growing trend in information technology. It will help the Federal Aviation Administration (FAA) meet the information-sharing needs of the Next Generation Air Traffic System (NGATS) and the federal government's E-government Initiative. SWIM will reduce the cost, complexity, and cycle time for building new applications and help the FAA implement SWIM-enabled applications that increase FAA and user productivity.  相似文献   
966.
The Scanning Sky Monitor is one of the experiments onboard the ASTROSAT, an Indian multiwavelength astronomy satellite mission. This experiment will detect and monitor X-ray transients in the energy band 2–10 keV. It is similar in design to the ASM on RXTE. It consists of position-sensitive proportional counters with one-dimensional mask. We describe the configuration of the experiment. We also discuss some of the results obtained using a detector which has already been fabricated and tested in our laboratory.  相似文献   
967.
The assessment of exposure to cosmic radiation on board aircraft is one of the preoccupations of organizations responsible for radiation protection. The cosmic radiation particle flux increases with altitude and latitude and depends on the solar activity. The radiation exposure has been estimated on several airlines using transatlantic, Siberian and transequatorial routes on board subsonic and supersonic aircraft, to illustrate the effect of these parameters. Measurements have been obtained with a tissue equivalent proportional counter using the microdosimetric technique. Data have been collected at maximum solar activity in 1991-92 and at minimum in 1996-98. The lowest mean dose rate measured was 3 microSv/h during a Paris-Buenos Aires flight in 1991; the highest was 6.6 microSv/h during a Paris-Tokyo flight using a Siberian route and 9.7 microSv/h on Concorde in 1996-97. The mean quality factor is around 1.8. The corresponding annual effective dose, based on 700 hours of flight for subsonic aircraft and 300 hours for Concorde, can be estimated between 2 mSv for least-exposed routes and 5 mSv for more exposed routes.  相似文献   
968.
A software tool for application in calculations of the position and velocity vectors of spacecraft is presented. These vectors are necessary at the stage of processing the data of space experiments and analyzing the results of ground-based observations. A new theory of satellite motion, in a numerical-analytical form, is the basis of this software application. The model of disturbing forces corresponds to recommendations of the International Laser Ranging Service. The reliability of the software tool is confirmed by the results of processing of high-precision laser satellite observations.  相似文献   
969.
This research aims to characterize regulation of the principal cytosolic protein kinases in maize, cultivar 'Merit' root tips, since much evidence indicates that stimuli which modulate the gravitropic response in this system act through regulation of activity of these enzymes. To this end, we have cloned a maize protein kinase belonging to a group of plant protein kinases with a catalytic domain similar in primary structure to the second messenger-regulated protein kinases known in animal and fungal systems. However, both the unique structural features conserved among plant protein kinases in this group, and lack of evidence for cyclic nucleotide signalling in plants point to operation of a novel protein kinase regulatory mechanism in plants. In order to test effects of possible regulators on protein kinase activity, we developed a sensitive method for detecting regulation of autophosphoryl labelling of protein kinases in unfractionated maize protein extracts. Regulation of protein kinase autophosphorylation in these extracts was different from that known in animals and fungi, further suggesting operation of unique protein kinase regulatory mechanisms in plants. Previous research has shown that light, or factors modulated by light, regulate plant protein kinase activity. We found that protein kinase activity was co-immunoprecipitated with the plant photoreceptor phytochrome, and was associated with phytochrome by high-affinity chemical interactions. Far-red reversibility of red-light regulation of phytochrome phosphorylation by the associated protein kinase indicates that it may modulate or transduce the light signals which lead to gravitropic sensitivity in 'Merit' maize.  相似文献   
970.
The development of the theory of high charge and energy (HZE) ion transport is reviewed. The basic solution behavior and approximation techniques will be described. An overview of the HZE transport codes currently available at the Langley Research Center will be given. The near term goal of the Langley program is to produce a complete set of one-dimensional transport codes. The ultimate goal is to produce a set of complete three-dimensional codes which have been validated in the laboratory and can be applied in the engineering design environment. Recent progress toward completing these goals is discussed.  相似文献   
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