Clover development during spaceflight: a model system.

The development of legume root nodules was studied as a model system for the examination of gravitational effects on plant root development. In order to examine whether rhizobial association with clover roots can be achieved in microgravity, experiments were performed aboard the KC-135 parabolic aircraft and aboard the sounding rocket mission Consort 3. Binding of rhizobia to roots and the initial stages of root nodule development successfully occurred in microgravity. Seedling germination experiments were performed in the sliding block device, the Materials Dispersion Apparatus, aboard STS-37. When significant hydration of the seeds was achieved, normal rates of germination and seedling development were observed.     

Carbon dioxide exchange of lettuce plants under hypobaric conditions.

Growth of plants in a Controlled Ecological Life Support System (CELSS) may involve the use of hypobaric pressures enabling lower mass requirements for atmospheres and possible enhancement of crop productivity. A controlled environment plant growth chamber with hypobaric capability designed and built at Ames Research Center was used to determine if reduced pressures influence the rates of photosynthesis (Ps) and dark respiration (DR) of hydroponically grown lettuce plants. The chamber, referred to as a plant volatiles chamber (PVC), has a growing area of about 0.2 m2, a total gas volume of about 0.7 m3, and a leak rate at 50 kPa of <0.1%/day. When the pressure in the chamber was reduced from ambient to 51 kPa, the rate of net Ps increased by 25% and the rate of DR decreased by 40%. The rate of Ps increased linearly with decreasing pressure. There was a greater effect of reduced pressure at 41 Pa CO2 than at 81 Pa CO2. This is consistent with reports showing greater inhibition of photorespiration (Pr) in reduced O2 at low CO2 concentrations. When the partial pressure of O2 was held constant but the total pressure was varied between 51 and 101 kPa, the rate of CO2 uptake was nearly constant, suggesting that low pressure enhancement of Ps may be mainly attributable to lowered partial pressure of O2 and the accompanying reduction in Pr. The effects of lowered partial pressure of O2 on Ps and DR could result in substantial increases in the rates of biomass production, enabling rapid throughput of crops or allowing flexibility in the use of mass and energy resources for a CELSS.     

A "cryptic" microbial mat: a new model ecosystem for extant life on Mars.

If life were present on Mars to day, it would face potentially lethal environmental conditions such as a lack of water, frigid temperatures, ultraviolet radiation, and soil oxidants. In addition, the Viking missions did not detect near-surface organic carbon available for assimilation. Autotrophic organisms that lived under a protective layer of sand or gravel would be able to circumvent the ultraviolet radiation and lack of fixed carbon. Two terrestrial photosynthetic near-surface microbial communities have been identified, one in the inter- and supertidal of Laguna Ojo de Liebre (Baja California Sur, Mexico) and one in the acidic gravel near several small geysers in Yellowstone National Park (Wyoming, U.S.A.). Both communities have been studied with respect to their ability to fix carbon under different conditions, including elevated levels of inorganic carbon. Although these sand communities have not been exposed to the entire suite of Martian environmental conditions simultaneously, such communities can provide a useful model ecosystem for a potential extant Martian biota.     

Two years of INTEGRAL result on X-ray binaries

INTEGRAL is the ESA lead International Gamma-Ray Astrophysical Laboratory, successfully launched the 17th October 2002 from Baikonur with a Proton vehicle. In view of the high sensitivity of the two γ-ray instruments IBIS and SPI and their capability to provide at the same time image, spectra and time profiles of all the sources in their wide field of view, a key project was approved as “Core Programme” to obtain deep observations of the Galactic Centre (GCDE) and to exploit regular scan of the whole Galaxy Plane since the beginning of the mission. This paper will briefly review the main astrophysical results obtained in the field of high energy Galactic sources with the INTEGRAL/IBIS γ-ray Imager onboard INTEGRAL, and make a comparison with the previous scenario depicted by the BeppoSAX and RXTE results.     

Analysis of the linear amplifier/analog-digital converter interfacein a digital microwave receiver

An analysis of the relationship between a linear amplifier chain and an analog-to-digital converter (ADC) in a digital microwave receiver, with respect to sensitivity and dynamic range issues, is presented. The effects of gain, third-order intermodulation products and ADC characteristics on the performance of the receiver are illustrated and design criteria for the linear amplifier chain (given a specified ADC) are developed. A computer program is included which calculates theoretical receiver performance based on gain and third-order intermodulation product selections. Experimental results are also presented and compared with theoretical values     

Space Station power requirements and issues

This paper provides an overview of the Space Station configuration and summarizes the requirements, architecture, and significant challenges associated with the electrical power system (EPS). The Space Station configuration was baselined during the systems design review (SDR) process in March, 1994. The current configuration includes the addition of Russia as an international partner, resulting in major changes to the assembly sequence, pressurized module complement, and overall power architecture. The Russian contributions to the power system architecture, as well as an-overview and development status of the US provided elements is presented. Finally, a planned flight demonstration of solar dynamic power system on the Mir as part of the first phase of US/Russian cooperation in human space flight is described     

FINDER, a system providing complex decision support for commercialtransport replanning operations

Decision-aid systems, likely to appear in future aircraft generations, could play a central role in the cockpit thanks to the broad spectrum of functionalities and decision support facilities they will offer to the crew. As part of such systems, the exploratory FINDER mock-up is a knowledge-based system (KBS) designed to help crew members continually optimize their flight plan by suggesting solutions considering exhaustive information related to flight context, either on pilot request or upon external information occurrence. The successful evaluation by AIR FRANCE pilots of that first mock-up dedicated to diversion procedure on pilot request has led to the current development of an enhanced system with nominal Enroute operations and real-time capabilities. Nominal Enroute operations concern the optimization with respect to an evolutive constraining or favouring environment (due to weather, traffic or regulated areas, ETOPS constraints). This study paves the way for a future Flight Assistant System concept which is already under investigation and may take place in SEXTANT Avionique's future development steps     

Biological degradation and composition of sweet potato biomass - errata

Many challenges are presented by biological degradation in a bioregenerative Controlled Ecological Life Support System as envisioned by the U.S. National Aeronautics and Space Administration. In studies conducted with biodegradative microorganisms indigenous to sweetpotato fields, it was determined that a particle size of 75 microns and incubation temperature of 30°C were optimal for degradation. The composition of the biomass and characterization of plant nutrient solution indicated the presence of potential energy sources to drive microbial transformations of plant waste. Selected indigenous soil isolates with ligno-cellulolytic or sulfate-reducing ability were utilized in biological studies and demonstrated diversity in their ability to reduce sulfate in solution and to utilize alternative carbon sources: a lignin analog 4-hydroxy, 3-methoxy cinnamic acid, cellulose, arabinose, glucose, sucrose, mannitol, galactose, ascorbic acid.     

Molecular analysis of mutagenesis by high LET radiation.

Mutation induction by high linear energy transfer [LET] alpha particles and gamma-rays was scored in the human hamster hybrid [AL] cells. Southern blotting technique was used to analyse the molecular changes in the DNA from both the HGPRT- and S1- mutants. Dose dependent mutagenesis in the AL cells irradiated with the charged particles was higher by almost 20 fold at the S1 than the corresponding HGPRT locus. Southern analysis of the mutants induced by the high LET particles showed mostly multilocus deletion at both the HGPRT and S1 genes.     

Specific effects of irradiance and CO2 concentration doublings on productivity and mineral content in lettuce.

Experiments in growth chambers with controlled atmosphere were performed to compare the effects on the productivity of two treatments stimulating photosynthesis: the doubling of CO2 concentration, the doubling of irradiance; the combining of both was also tested. A large effect of light was noticed: (i) the accumulation of carbon was, contrarily to CO2 effect, amplified within time, and led to the most important dry matter production. (ii) the specific leaf weight was about two-fold increased. (iii) the nitrate content was 2-3 fold less. A significant positive effect of CO2 was detected on the fresh biomass production and the iron content of lettuce. A synergy was observed on dry matter production by the interaction of the two factors.