Physiological, biochemical and molecular processes associated with gravitropism in roots of maize.

This research aims to characterize regulation of the principal cytosolic protein kinases in maize, cultivar 'Merit' root tips, since much evidence indicates that stimuli which modulate the gravitropic response in this system act through regulation of activity of these enzymes. To this end, we have cloned a maize protein kinase belonging to a group of plant protein kinases with a catalytic domain similar in primary structure to the second messenger-regulated protein kinases known in animal and fungal systems. However, both the unique structural features conserved among plant protein kinases in this group, and lack of evidence for cyclic nucleotide signalling in plants point to operation of a novel protein kinase regulatory mechanism in plants. In order to test effects of possible regulators on protein kinase activity, we developed a sensitive method for detecting regulation of autophosphoryl labelling of protein kinases in unfractionated maize protein extracts. Regulation of protein kinase autophosphorylation in these extracts was different from that known in animals and fungi, further suggesting operation of unique protein kinase regulatory mechanisms in plants. Previous research has shown that light, or factors modulated by light, regulate plant protein kinase activity. We found that protein kinase activity was co-immunoprecipitated with the plant photoreceptor phytochrome, and was associated with phytochrome by high-affinity chemical interactions. Far-red reversibility of red-light regulation of phytochrome phosphorylation by the associated protein kinase indicates that it may modulate or transduce the light signals which lead to gravitropic sensitivity in 'Merit' maize.     

Performance and reliability of the NASA Biomass Production Chamber.

The Biomass Production Chamber (BPC) at the Kennedy Space Center is part of the Controlled Ecological Life Support System (CELSS) Breadboard Project. Plants are grown in a closed environment in an effort to quantify their contributions to the requirements for life support. Performance of this system is described. Also, in building this system, data from component and subsystem failures are being recorded. These data are used to identify problem areas in the design and implementation. The techniques used to measure the reliability will be useful in the design and construction of future CELSS. Possible methods for determining the reliability of a green plant, the primary component of a CELSS, are discussed.     

Galactic cosmic ray transport methods: past, present, and future.

The development of the theory of high charge and energy (HZE) ion transport is reviewed. The basic solution behavior and approximation techniques will be described. An overview of the HZE transport codes currently available at the Langley Research Center will be given. The near term goal of the Langley program is to produce a complete set of one-dimensional transport codes. The ultimate goal is to produce a set of complete three-dimensional codes which have been validated in the laboratory and can be applied in the engineering design environment. Recent progress toward completing these goals is discussed.     

Oscillations of axial plant organs.

The tips of roots and shoots commonly show lateral movements as they grow forwards. These occur as both circumnutations (with long periods and large amplitudes) and micronutations (with short periods and small amplitudes). Their properties are reviewed, with emphasis on roots, and possible ways in which they could be regulated are discussed. The mechanisms could include long-range controls (for circumnutations) that depend on transmissible signals using steps common to gravitropism, and short-range controls (for micronutations) that operate within the elongation zone. The former are a property of the apex as a whole, while the latter may be confined to localized groups of cells. Simulation of nutations is presented with a view to isolating key physiological processes. However, this approach is limited by the current inadequate understanding of the growth mechanisms involved.     

A program to measure new energetic particle nuclear interaction cross sections.

The Transport Collaboration, consisting of researchers from institutions in France, Germany, Italy and the USA, has established a program to make new measurements of nuclear interaction cross sections for heavy projectiles (Z > or = 2) in targets of liquid H2, He and heavier materials. Such cross sections directly affect calculations of galactic and solar cosmic ray transport through matter and are needed for accurate radiation hazard assessment. To date, the collaboration has obtained data using the LBL Bevalac HISS facility with 20 projectiles from 4He to 58Ni in the energy range 393-910 MeV/nucleon. Preliminary results from the analysis of these data are presented here and compared to other measurements and to cross section prediction formulae.     

Suboptimal joint probabilistic data association

A significant problem in multiple target tracking is the hit-to-track data association. A hit is a received signal from a target or background clutter which provides positional information. If an incorrect hit is associated with a track, that track could diverge and terminate. Prior methods for this data association problem include various optimal and suboptimal two-dimensional assignment algorithms which make hit-to-track associations. Another method is to assign a weight for the reasonable hits and use a weighted centroid of those hits to update the track. The method of weighting the hits is known as joint probabilistic data association (JPDA). The authors review the JPDA approach and a simple ad hoc approximation and then introduce a new suboptimal JPDA algorithm. Examples which compare an optimal two-dimensional assignment algorithm with the ad hoc and the new suboptimal JPDA formulation are given     

Target motion analysis with multiple arrays: performance analysis

The performance analysis of source trajectory estimation using measurements provided by multiple platforms (or arrays) is studied. In numerous practical situations, the maneuvering ability of the receiver (e.g., a ship towing linear arrays) is limited, leading to the assumption that the observer motion is rectilinear and uniform. Even if this hypothesis appears quite restrictive, practical and tactical considerations fully justify its interest. This leads to consider multiple (platform) target motion analysis (denoted MTMA) and to analyze the performance of such trajectory estimation methods     

A baseband Doppler simulator for radar system development

Elementary electronics building blocks can be configured as a low-cost baseband Doppler simulator for developing the post-detector processing parts such as MTIs and MTDS of millimeter wave pulsed radars employing amplitude and phase channels. The challenge of narrow pulses in conjunction to low Doppler frequencies is handled here by CMOS switches and normal function generator ICs. Test noise is generated with an off-the-shelf radio RF/IF circuit. Wideband video amplifiers are not required. Typical performance figures include pulse widths from 50 ns, target ranges from 0 to ambiguity maximum, target velocities from 0.5 m/s to 500 m/s and PRFs up to 100 kflz. Virtual target RCS can be adjusted as needed in a straightforward way.     

A fault-tolerant air data/inertial reference unit

The fault-tolerant air data/inertial reference unit (ADIRU) described is a key part of a fault-tolerant air data/inertial reference system (ADIRS) designed to be the inertial and air data reference for the ARINC 651 Integrated Modular Avionics (IMA) distributed architecture. The ADIRU has been designed to meet the commercial aviation market demands for low life cycle cost and high integrity fault detection, fault isolation, and redundancy management. The ADIRU's internal redundant resources provide quad channel redundancy that is one level higher than conventional triple redundant systems, allowing it to provide deferred maintenance capability. Robust partitioning, simple serial internal interfaces, and simple voting planes ensure that internal redundant components are properly utilized to provide high integrity system outputs. This relieves systems using it from having to perform their own redundancy management of the air data and inertial outputs from multiple sources required by conventional systems     

A two-dimensional position-sensitive spectroscopic proportional counter for balloon-borne hard X-ray astronomy

A new design of position sensitive spectroscopic proportional counter is described, for use in a balloon borne hard x-ray telescope. Initial position and spectral resolution data from a one-dimensional laboratory prototype are reported. With this device, the final telescope will have an angular resolution of better than 10 minutes of arc.