Variations of the gravitational field as a motive power for rhythmics of biochemical processes.

Variations of the gravitational field affected by the Sun and the Moon while the Earth's moving along the orbit seem to be a powerful source of many rhythmical processes typical of biochemical processes. Studies carried out in AARI revealed the obvious relationships between the dynamics of some biochemical reactions and lambda(D)-function describing the regular variations of the gravitational field under combined influence of the Sun and the Moon. The following of them are examined as examples: the rate of the unithiol oxidation in vitro, concentration of the thiol compounds in human urine, some hematological indicators (rate of the erythrocytes sedimentation, hemoglobin content). Compatibility of run of the biochemical indicators and lambda(D)-function is indicative of essential influence of the regular variations of the gravitational field on rhythmics of the biochemical processes. As this takes place, the solar activity acts like to the instability factor. Balance of the solar activity effects and the varying gravitational field effect alter in time depending on location in the solar activity cycle.     

Einstein X-ray observations of cataclysmic variables

Observations with the imaging X-ray detectors on the Einstein Observatory have led to a large increase in the number of low luminosity X-ray sources known to be associated with cataclysmic variable stars (CVs). The high sensitivity of the Einstein instrumentation has permitted study of their short timescale variability and spectra. The data are adding significantly to our knowledge of the accretion process in cataclysmic variables and forcing some revision in our ideas concerning the origin of the optical variability in these stars.     

Instrumentation and data systems for high-time resolution study of space plasma shocks

This paper deals with the principal methods of achieving high-time resolution measurements for the study of fine structure of shocks and other discontinuities in space plasmas. In the measurements of ion energy spectra, we have obtained the time resolution about 1s.In the Soviet-Czechoslovak INTERSHOCK project we will obtain temporal resolution better than 0.1s in the measurement of the main plasma parameters. This will be obtained with the multichannel energy spectrometer and the on-board data acquisition and processing system triggered by the shock signature. This system controls the data sampling rate from some scientific instruments and switches on a high-sampling rate near the shock. The method implies detecting a shock by means of on-board processing of magnetic field and plasma parameters. The algorithm for this detection uses both parameters separately as well as in combinations.     

Experiment on the <Emphasis Type="Italic">Vernov</Emphasis> satellite: Transient energetic processes in the Earth’s atmosphere and magnetosphere. Part I: Description of the experiment

The program of physical studies on the Vernov satellite launched on July 8, 2014 into a polar (640 × 830 km) solar-synchronous orbit with an inclination of 98.4° is presented. We described the complex of scientific equipment on this satellite in detail, including multidirectional gamma-ray detectors, electron spectrometers, red and ultra-violet detectors, and wave probes. The experiment on the Vernov satellite is mainly aimed at a comprehensive study of the processes of generation of transient phenomena in the optical and gamma-ray ranges in the Earth’s atmosphere (such as high-altitude breakdown on runaway relativistic electrons), the study of the action on the atmosphere of electrons precipitated from the radiation belts, and low- and high-frequency electromagnetic waves of both space and atmospheric origin.     

Clinical effects of thigh cuffs during a 7-day 6 degrees head-down bed rest.

Thigh cuffs are used by Russian cosmonauts to limit the fluid shift induced by space flight. A ground simulation using the head-down bed rest (HDBR) model was performed to assess the effects of thigh cuffs on clinical tolerance and orthostatic adaptation. 8 male healthy volunteers (32.4 +/- 1.9 years) participated twice in a 7-day HDBR--one time with thigh cuffs (worn daily from 9 am to 7 pm) (TC) and one time without (WTC). Orthostatic tolerance was assessed by a 10 minute stand test and by a LBNP test (5 min at -15, -30, -45 mmHg) before (BDC-1) and at the end of the HDBR period (R+1). Plasma volume was measured before and at the end of HDBR by the Evans blue dye dilution technique. Thigh cuffs limits headache due to fluid shift, as well as the loss in plasma volume (TC: -5.85 +/- 0.95%; WTC: -9.09 +/- 0.82%, p<0.05). The mean duration of the stand test (R+1) did not differ in the two group (TC 7.1 +/- 1.3 min; WTC 7.0 +/- 1.0 min). The increase in HR and decrease in diastolic blood pressure were slightly but significantly larger without thigh cuffs. Duration of the LBNP tests did not differ with thigh cuffs. Thigh cuffs limit the symptoms due to fluid shift and the loss in plasma volume. They partly reduced the increase in HR during orthostatic stress but had no effect on duration of orthostatic stress tests.     

Forecasting of DST variations from polar cap indices using neural networks

The 15-min averaged polar cap (PC) index was used as an input parameter for the Dst variation forecasting. The PC index is known to describe well the principal features of the solar wind as well as the total energy input to the magnetosphere. This allowed us to design a neural network able to forecast the Dst variations from 1 to 4 h ahead. 1998 PC and Dst data sets were used for training and testing and 1997 data sets was used for validation proposes. From the 15 moderate and strong geomagnetic storms observed during 1997, nine were successfully forecasted. In three cases the observed minimum Dst value was less than the predicted one, and only in three cases the neural network was not able to reproduce the features of the geomagnetic storm.     

The effects of extended clinorotation on potato minituber formation and structure.

Formation and structure of potato minitubers grown aseptically for 30 days on a horizontal clinostat and in stationary control have been studied by light and electron microscopy. It was demonstrated that the number of plants that formed minitubers, their size and fresh weight, was higher when clino-rotated than in the stationary control. It was revealed that the amount of amyloplasts in parenchyma cell sections was doubled in minitubers formed under clino-rotation. Other factors (shape of minitubers and size of reserve parenchyma cells) did not differ from the stationary control. The changes in amyloplast ultrastructure suggest accelerated cell maturity of potato reserve parenchyma in extended clino-rotation.     

Distortions of rectangular pulse with linear frequency modulation during trans-ionospheric sounding of the Martian surface

Specific features of propagation of a wideband rectangular pulse along the route spacecraft-Martian surface-spacecraft caused by the influence of the planetary ionosphere are considered as applied to the problem of radio pulse sounding of the subsurface Martian soil from the Mars-Express satellite. The night Martian ionosphere considerably reduces the energy of the pulse, but does not lead to degradation of its envelope or uncertainty function. When sounding a two-layer surface, the influence of the ionosphere is also manifested in limitation from below of the thickness of the upper layer accessible for measurement, which is more essential than when sounding with the use of a wideband Gaussian pulse. It is demonstrated that the surface sounding is possible through the dayside planet ionosphere at the parameters of operating pulse of the MARSIS radar.     

Induction of hypoxic root metabolism results from physical limitations in O2 bioavailability in microgravity.

Numerous spaceflight experiments have noted changes in the roots that are consistent with hypoxia in the root zone. These observations include general ultrastructure analysis and biochemical measurements to direct measurements of stress specific enzymes. In experiments that have monitored alcohol dehydrogenase (ADH), the data shows this hypoxically responsive gene is induced and is associated with increased ADH activity in microgravity. These changes in ADH could be induced either by spaceflight hypoxia resulting from inhibition of gravity mediated O2 transport, or by a non-specific stress response due to inhibition of gravisensing. We tested these hypotheses in a series of two experiments. The objective of the first experiment was to determine if physical changes in gravity-mediated O2 transport can be directly measured, while the second series of experiments tested whether disruption of gravisensing can induce a non-specific ADH response. To directly measure O2 bioavailability as a function of gravity, we designed a sensor that mimics metabolic oxygen consumption in the rhizosphere. Because of these criteria, the sensor is sensitive to any changes in root O2 bioavailability that may occur in microgravity. In a KC-135 experiment, the sensor was implanted in a moist granular clay media and exposed to microgravity during parabolic flight. The resulting data indicated that root O2 bioavailability decreased in phase with gravity. In experiments that tested for non-specific induction of ADH, we compared the response of transgenic Arabidopsis plants (ADH promoted GUS marker gene) exposed to clinostat, control, and waterlogged conditions. The plants were grown on agar slats in a growth chamber before being exposed to the experimental treatments. The plants were stained for GUS activity localization, and subjected to biochemical tests for ADH, and GUS enzyme activity. These tests showed that the waterlogging treatment induced significant increases in GUS and ADH enzyme activities, while the control and clinostat treatments showed no response. This work demonstrates: (1) the inhibition of gravity-driven convective transport can reduce the O2 bioavailability to the root tip, and (2) the perturbation of gravisensing by clinostat rotation does not induce a nonspecific stress response involving ADH. Together these experiments support the microgravity convection inhibition model for explaining changes in root metabolism during spaceflight.