Tolerance of anhydrobiotic eggs of the Tardigrade Ramazzottius varieornatus to extreme environments

Tardigrades are tiny (less than 1?mm in length) invertebrate animals that have the potential to survive travel to other planets because of their tolerance to extreme environmental conditions by means of a dry ametabolic state called anhydrobiosis. While the tolerance of adult tardigrades to extreme environments has been reported, there are few reports on the tolerance of their eggs. We examined the ability of hydrated and anhydrobiotic eggs of the tardigrade Ramazzottius varieornatus to hatch after exposure to ionizing irradiation (helium ions), extremely low and high temperatures, and high vacuum. We previously reported that there was a similar pattern of tolerance against ionizing radiation between hydrated and anhydrobiotic adults. In contrast, anhydrobiotic eggs (50% lethal dose; 1690 Gy) were substantially more radioresistant than hydrated ones (50% lethal dose; 509 Gy). Anhydrobiotic eggs also have a broader temperature resistance compared with hydrated ones. Over 70% of the anhydrobiotic eggs treated at either -196°C or +50°C hatched successfully, but all the hydrated eggs failed to hatch. After exposure to high-vacuum conditions (5.3×10(-4) Pa to 6.2×10(-5) Pa), the hatchability of the anhydrobiotic eggs was comparable to that of untreated control eggs.     

Overview of Differential VLBI Observations of Lunar Orbiters in SELENE (Kaguya) for Precise Orbit Determination and Lunar Gravity Field Study

The Japanese lunar explorer SELENE (Kaguya), which was launched on September 14th, 2007, was the target of VLBI observations over the period November 2007 to June 2009. These observations were made in order to improve the lunar gravity field model, in particular the lower degree coefficients and the model near the limb. Differential VLBI Radio sources, called VRAD instruments, were on-board the subsatellites, Rstar (Okina) and Vstar (Ouna), and the radio signals were observed by the Japanese VERA (VLBI Exploration of Radio Astrometry) network, and an international VLBI network. Multi-frequency and same-beam VLBI techniques were utilized and were essential aspects of the successful observing program. Multi-frequency VLBI was employed in order to improve the accuracy of the orbit determination obtained from the phase delay from the narrow-band satellite signals, while the same-beam VLBI method was used to resolve the cycle ambiguity which is inherent in the multi-frequency VLBI method. The observations were made at three S-band frequencies (2212, 2218 and 2287 MHz), and one X-band frequency (8456 MHz). We have succeeded in correlating the recorded signals from Okina/Ouna, and we obtained phase delays with an accuracy of several pico-seconds at S-band.