In situ microbial detection in Mojave Desert soil using native fluorescence

We report on the use of a portable instrument for microbial detection in the Mojave Desert soil and the potential for its use on Mars. The instrument is based on native fluorescence and employs four excitation wavelengths combined with four emission wavelengths. A soil dilution series in which known numbers of Bacillus subtilis spores were added to soil was used to determine the sensitivity of the instrument. We found that the fluorescence of the biological and organic components of the desert soil samples studied can be as strong as the fluorescence of the mineral component of these soils. Using the calibration derived from B. subtilis spores, we estimated that microbial content at our primary sampling site was 10(7) bacteria per gram of soil, a level confirmed by phospholipid fatty acid analysis. At a nearby site, but in a slightly different geological setting, we tested the instrument's ability to map out microbial concentrations in situ. Over a ～50 m diameter circle, soil microbial concentrations determined with the B. subtilis calibration indicate that the concentrations of microorganisms detected varies from 10(4) to 10(7) cells per gram of soil. We conclude that fluorescence is a promising method for detecting soil microbes in noncontact applications in extreme environments on Earth and may have applications on future missions to Mars.     

Positive identification of aircraft on airport movementarea-results of FAA trials

Current operational airport surface surveillance systems do not positively identify aircraft as unique targets. Air traffic controllers are instead presented with a primary radar picture showing all traffic on the airport movement area. The Federal Aviation Administration (FAA) is evaluating different types of systems that will add the aircraft's call-sign or flight number to the radar image thereby allowing controllers to positively identify individual aircraft at all times. This paper describes the development, implementation, and testing of the airport surface target identification system (ATIDS), and presents results of initial trials conducted at Atlanta Hartsfield International Airport     

Quantitative electrocardiography during extended space flight

To assess the effects of prolonged space flight on the electrophysiological properties of the heart, vectorcardiograms (VCG) were obtained on the Skylab crews at regular intervals during flight and the pre- and postflight periods. The VCG signals were telemetered from Skylab and analyzed by digital computer. Conventional 12-lead electrocardiograms were derived from the VCG signals by a lead transformation program. Standardized exercise loads were incorporated into the experiment protocol to increase the sensitivity of the VCG for effects of deconditioning and to detect susceptibility for arrhythmias. In Skylab II, 24 preflight, 21 inflight, and 19 postflight experiments were analyzed. Statistically significant inflight changes observed in two or more crew members included: decreased resting heart rate, increased QRS duration, anterior shift QRS vector, increased QRS vector magnitude, anterior shift T vector, and increased T vector magnitude. One astronaut had occasional premature ventricular contractions (PVC) during the pre- and postflight phases. He had a single episode of multiple PVC's during heavy-load exercise testing in flight. A second astronaut had no arrhythmia during pre- or inflight testing. On postflight day 21 he had multiple PVC's and salvos of ectopic ventricular beats. He has had no recurrence of the arrhythmia. With the exception of the cardiac arrhythmias, no deleterious electrophysiological changes were observed during Skylab II.     

Processing of filtered GPS data

General sufficiency conditions are developed for the stability of a discrete-time cascaded-filter architecture. An application of the result is in the filtering of prefiltered Global Positioning System (GPS) data during times in which the GPS receiver is tracking less than four satellites. Simulation results are presented for the case where the master filter processes the GPS filter estimate after the GPS filter attains uniform complete observability and controllability following its third filter cycle as well as the case where the observability condition is not met     

Attitude Control of a Skylark Rocket Using Magnetometers and a Moon Sensor

The problems of attitude control of a Skylark rocket are considered on the specific occasion of a night flight during which, of course, the sun was not available as a pointing vector. Pointing was achieved using magnetometers, while roll control was obtained using a suitably placed moon sensor. The logical sequence, as well as the electronic solution, that insured the acquisition of the correct payload orientation is presented.     

Optimum Filters for Image Registration

Two-dimensional cross-correlation techniques are applied to the problem of image registration under the assumption of small geometric distortion. Optimum filter functions for use with arbitrary window functions are derived for two performance measures of interest: peak-to-sidelobe ratio, and mean- square registration error; the latter is examined in terms of the contribution due to distortion and the contribution due to noise. A generalized Lagrange multiplier approach is used to derive at least approximate ate solutions to the case where the images are modeled as random processes and to the case where the images are assumed to be deterministic.     

Solar flux and its variations

We present data on the solar irradiance as derived from a number of sources. An attempt was made to bring these data onto a uniform scale. The results are presented in Table IV and Figure 6. Summation of fluxes at all wavelengths yields a figure of 1357.826 W m^-2 for the solar constant. Estimates are made of the solar flux variations due to flares, active regions (slowly varying component), solar rotation and the 11-year cycle.Solar activity does not produce a significant variation in the value of the solar constant. Nevertheless, variations in the X-ray and extreme ultraviolet portions of the solar flux may be several orders of magnitude during solar activity, especially at times of major flares. It is well established that these short wavelength flux enhancements cause significant changes in the terrestrial ionosphere.