Gravisensing: ionic responses, cytoskeleton and amyloplast behavior.

In Zea mays L., changes in orientation of stems are perceived by the pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. Gravity is perceived in the bundle sheath cells, which contain amyloplasts that sediment to the new cell base when a change in the gravity vector occurs. The mechanism by which the mechanical signal is transduced into a physiological response is so far unknown for any gravity perceiving tissue. It is hypothesized that this involves interactions of amyloplasts with the plasma membrane and/or ER via cytoskeletal elements. To gain further insights into this process we monitored amyloplast movements in response to gravistimulation. In a pharmacological approach we investigated how the dynamics of plastid sedimentation are affected by actin and microtubule (MT) disrupting drugs. Dark grown caulonemal filaments of the moss Physcomitrella patens respond to gravity vector changes with a reorientation of tip growth away from the gravity vector. MT distributions in tip cells were monitored over time and MTs were seen to accumulate preferentially on the lower flank of the tip 30 min after a 90 degree turn. Using a self-referencing Ca2+ selective ion probe, we found that growing caulonemal filaments exhibit a Ca2+ influx at the apical dome, similar to that reported previously for other tip growing cells. However, in gravistimulated Physcomitrella filaments the region of Ca2+ influx is not confined to the apex, but extends about 60 micrometers along the upper side of the filament. Our results indicate an asymmetry in the Ca2+ flux pattern between the upper and side of the filament suggesting differential activation of Ca2+ permeable channels at the plasma membrane.     

Analysis of the Cyclotron Facility calibration and aircraft dosimetry results from the LIULIN-3M instrument.

The LIULIN-3M instrument is a further development of the LIULIN dosimeter-radiometer, used on the MIR spacestation during the 1988-1994 time period. The LIULIN-3M is designed for continuous monitoring of the radiation environment during the BION-12 satellite flight in 1999. A semiconductor detector with 1 mm thickness and cm2 area is contained in the instrument. Pulse high analysis technique is used to determine the energy losses in the detector. The final data from the instrument are the flux and the dose rate for the exposure time and 256 channels of absorbed dose spectra based on the assumption that the particle flux is normal to the detector. The LIULIN-3M instrument was calibrated by proton fluxes with different energies at the Indiana University Cyclotron Facility in June 1997 and had been used for radiation measurements during commercial aircraft flights. The calibration procedure and some flight results are presented in this paper.     

Particle trajectories in seeds of Lactuca sativa and chromosome aberrations after exposure to cosmic heavy ions on Cosmos Biosatellites 8 and 9.

The potentially specific importance of the heavy ions of the galactic cosmic radiation for radiation protection in manned spaceflight continues to stimulate in situ, i.e., spaceflight experiments to investigate their radiobiological properties. Chromosome aberrations as an expression of a direct assault on the genome are of particular interest in view of cancerogenesis being the primary radiation risk for man in space. In such investigations the establishment of the geometrical correlation between heavy ions' trajectories and the location of radiation sensitive biological substructures is an essential task. The overall qualitative and quantitative precision achieved for the identification of particle trajectories in the order of approximately 10 micrometers as well as the contributing sources of uncertainties are discussed. We describe how this was achieved for seeds of Lactuca sativa as biological test organisms, whose location and orientation had to be derived from contact photographies displaying their outlines and those of the holder plates only. The incidence of chromosome aberrations in cells exposed during the COSMOS 1887 (Biosatellite 8) and the COSMOS 2044 (Biosatellite 9) mission was determined for seeds hit by cosmic heavy ions. In those seeds the incidence of both single and multiple chromosome aberrations was enhanced. The results of the Biosatellite 9 experiment, however, are confounded by spaceflight effects unrelated to the passage of heavy ions.     

Oxygen Isotopes in the Solar System

The oxygen three-isotope system has major advantages over the two-isotope systems of hydrogen, carbon, and nitrogen because different fractionation laws govern intraplanetary and interplanetary processes. This permits discriminating between solar nebular processes and parent-body processes. Oxygen isotopes also serve as a sensitive natural tracer for meteorite classification. This revised version was published online in August 2006 with corrections to the Cover Date.     

A program to measure new energetic particle nuclear interaction cross sections.

The Transport Collaboration, consisting of researchers from institutions in France, Germany, Italy and the USA, has established a program to make new measurements of nuclear interaction cross sections for heavy projectiles (Z > or = 2) in targets of liquid H2, He and heavier materials. Such cross sections directly affect calculations of galactic and solar cosmic ray transport through matter and are needed for accurate radiation hazard assessment. To date, the collaboration has obtained data using the LBL Bevalac HISS facility with 20 projectiles from 4He to 58Ni in the energy range 393-910 MeV/nucleon. Preliminary results from the analysis of these data are presented here and compared to other measurements and to cross section prediction formulae.     

A two-dimensional position-sensitive spectroscopic proportional counter for balloon-borne hard X-ray astronomy

A new design of position sensitive spectroscopic proportional counter is described, for use in a balloon borne hard x-ray telescope. Initial position and spectral resolution data from a one-dimensional laboratory prototype are reported. With this device, the final telescope will have an angular resolution of better than 10 minutes of arc.     

On the mechanisms of cooling of the nightside thermosphere of venus

The plausible mechanisms of cooling of the nightside Venus' thermosphere are analysed with the aid of the model of the atmospheric heat budget that incorporates, in addition to thermal conduction and IR radiation in the 15 μ band of CO₂, heating and cooling due to global scale winds, eddy turbulence, and IR radiation in the rotational bands of H₂O and CO, as well as the 63 μ line of atomic oxygen. The H₂O mixing ratio and parameters of turbulence required for cooling of the thermosphere down to the observed low temperatures are evaluated.     

Trapped antiprotons produced by cosmic rays in the Earth's magnetosphere.

The existence of significant fluxes of antiparticles in the Earth magnetosphere has been predicted on theoretical considerations in this article. These antiparticles (positrons or antiprotons) at several hundred kilometers of altitudes, we believe are not of direct extraterrestrial origin, but are the natural products of nuclear reactions of the high energy primary cosmic rays (CR) and trapped protons (TP) confined in the terrestrial radiation belt, with the constituents of terrestrial atmosphere. Extraterrestrial positrons and antiprotons born in nuclear reactions of the same CR particles passing through only 5-7 g/cm2 of interstellar matter, exhibit lower fluxes compared to the antiprotons born at hundreds of g/cm2 in the atmosphere, which when confined in the magnetic field of the Earth (in any other planet), get accumulated. We present the results of the computations of the antiproton fluxes at 10 MeV to several GeV energies due to CR particle interactions with the matter in the interstellar space, and also with the residual atmosphere at altitudes of approximately 1000 km over the Earth's surface. The estimates show that the magnetospheric antiproton fluxes are greater by two orders of magnitude compared to the extraterrestrial fluxes measured at energies <1-2 GeV.     

Quaternion regularization in celestial mechanics and astrodynamics and trajectory motion control. I

Regularization problems in celestial mechanics and astrodynamics are considered. The fundamental regular quaternion models of celestial mechanics and astrodynamics are presented. It is shown that the efficiency of analytical investigation and numerical solution of boundary problems of optimal trajectory motion control of spacecraft may be increased using quaternion astrodynamics models. The regularization problem of celestial mechanics and astrodynamics that implies eliminating the feature, which arises in the equations of the two-body problem in case of impact of the second body with the central body, is considered in the first section of the paper. The quaternion method for regularizing the equations of the perturbed spatial two-body problem suggested by the author is presented; the method is compared with Kustaanheimo-Stiefel (KS) regularization. Demonstrative geometric and kinematic interpretations of regularizing transformations are provided. Regular quaternion equations for the two-body problem, which generalize the regular Kustaanheimo-Stiefel equations, as well as regular equations in quaternion osculating elements and quaternion regular equations for perturbed central motion of a material point, are considered. The papers on quaternion regularization in celestial mechanics and astrodynamics are briefly analyzed.