Experimental evaluation of the processes resulting from the introduction of the transgenic microorganism Escherichia coli Z905/pPHL7 (lux+) into aquatic microcosms.

The processes resulting from the introduction of the tranagenic microorganism (TM) E. coli Z905/pPHL7 into aquatic microcosms have been modeled experimentally. It has been shown that the TM E. coli is able to adapt to a long co-existence with indigenous heterotrophic microflora in variously structured microcosms. In more complex microcosms the numerical dynamics of the introduced E. coli Z905/pPHL7 population is more stable. In the TM populations staying in the microcosms for a prolonged time, changes are recorded in the phenotypic expression of plasmid genes (ampicillin resistance and the luminescence level) and chromosome genes (morphological and physiological traits). However, in our study microcosms, the recombinant plasmid persisted in the TM cells for 6 years after the introduction, and as the population adapts to the conditions of the microcosms, the efficiency of the cloned gene expression in the cells is restored. In the microcosms with high microalgal counts (10(7) cells/ml), cells with a high threshold of sensitivity to ampicillin dominate in the population of the TM E. coli Z905/pPHL7.     

Magnetic resonance image enhancement using V-filter

A method for enhancing the boundaries of magnetic resonance images (MRIs) using a V-filter (a spatial nonlinear filter) is presented. By iterated V-filtering the signal-to-noise ratio of the MRI can be increased while preserving edge sharpness. A simulation experiment was carried out to evaluate the filter, using clinical brain images. The boundaries of the brain tumor and edematous region were precisely extracted by the region segmentation techniques     

Effects of altered gravity on the swimming behaviour of fish.

Humans taking part in parabolic aircraft flights (PAFs) may suffer from space motion sickness-phenomena (SMS, a kinetosis). It has been argued that SMS during PAFs might not be based on microgravity alone but rather on changing accelerations from 0 g to 2 g. We test here the hypothesis that PAF-induced kinetosis is based on asymmetric statoliths (i.e., differently weighed statoliths on the right and the left side of the head), with asymmetric inputs to the brain being disclosed at microgravity. Since fish frequently reveal kinetotic behaviour during PAFs (especially so-called spinning movements and looping responses), we investigated (1) whether or not kinetotically swimming fish at microgravity would have a pronounced inner ear otolith asymmetry and (2) whether or not slow translational and continuously changing linear (vertical) acceleration on ground induced kinetosis. These latter accelerations were applied using a specially developed parabel-animal-container (PAC) to stimulate the cupular organs. The results suggest that the fish tested on ground can counter changing accelerations successfully without revealing kinetotic swimming patterns. Kinetosis could only be induced by PAFs. This finding suggests that it is indeed microgravity rather than changing accelerations, which induces kinetosis. Moreover, we demonstrate that fish swimming kinetotically during PAFs correlates with a higher otolith asymmetry in comparison to normally behaving animals in PAFs.     

Organic solids produced from simple C/H/O/N ices by charged particles: applications to the outer solar system.

CH4, CO, and CO2 are all potential one-carbon molecular repositories in primitive icy objects. These molecules are all found in the Comet Halley coma, and are probable but, (except for CH4 detected on Triton and Pluto) undetected subsurface constituents in icy outer solar system objects. We have investigated the effects of charged particle irradiation by cold plasma discharge upon surfaces of H2O:CH4 clathrate having a 200:1 ratio, as well as upon ices composed of H2O plus C2H6 or C2H2 (sometimes plus NH3) which are also plausible constituents. These materials color and darken noticeably after a dose 10(9) - 10(10) erg cm-2, which is deposited rapidly (< or = 10(4) yr.) in solar system environments. The chromophore is a yellowish to tan organic material (a tholin) which we have studied by UV-VIS reflection and transmission, and IR transmission spectroscopy. Its yield, -1 C keV-1, implies substantial production of organic solids by the action of cosmic rays and radionuclides in cometary crusts and interiors, as well as rapid production in satellite surfaces. This material shows alkane bands which Chyba and Sagan have shown to well match the Halley infrared emission spectrum near 3.4 microns, and also bands due to aldehyde, alcohol and perhaps alkene/aromatic functional groups. We compare the IR spectral properties of these tholins with the spectra of others produced by irradiation of gases and ices containing simple hydrocarbons.     

Kinetic theory in aerothermodynamics

Aerothermodynamics is a branch of gas dynamics which is usually considered for continuum media. But many problems of gas dynamics cannot be solved and even properly understood in the framework of continuum media, and cannot be described on the macroscopic level. The aim of this review is to consider some problems of a continuum flow regime which cannot be solved without regarding the gas as a collection of molecules. Some of these problems are important for aerothermodynamics today. Others open up new ways, new possibilities and new questions.     

Transfer orbit stage gyrocompass alignment algorithm twist and swayenvironment for Mar's Observer mission on commercial Titan

The problems presented by the launch of a booster vehicle with a strapdown system on an expandable launch vehicle are addressed. These include the Earth motion by the wind-induced twist and sway motion at the launch pad, vibration-induced motion, and various sensor errors, as well as the limited memory and processing power of the flight computer. A robust numeric gyrocompass alignment algorithm is presented, and its performance in this environment is examined     

Solar variability effects studied by tree-ring data wavelet analysis

The global change approach to study the Sun-Earth system gives a growing amount of evidences that climate dynamics is affected by a large number of factors. The solar variability is very likely to be among them. Natural records, such as tree ring data, can be investigated to study the past global and regional climate, which was influenced by the solar radiative output variations, associated to solar activity. Wavelet transform analysis was applied to sunspot number and tree ring width time series from 1837 to 1996 at Concórdia, Brazil. The amplitude and cross-amplitude spectral representation in the time-frequency domain allowed us to detect the occurrence of predominant periodicities and the relationship between the sunspot number and the tree ring time series. The Morlet complex wavelet analysis was used to study the most important variability factors on time scales ranging from from 2 to 100 years, and their stability in time, which is shown in both time series studied. We also applied the cross-wavelet spectral analysis to evaluate time delay among different tree ring time series, and between tree ring and sunspot number time series.     

Amphibian tail regeneration in space: effect on the pigmentation of the blastema.

In Urodele amphibians, the tail regenerates after section. This regeneration, including tissues as different as bone (vertebrae), muscle, epidermis and central nervous system (spinal cord), was studied in adult Pleurodeles sent aboard the russian satellite Bion 10 and compared with tail regeneration in synchronous controls. Spinal cord, muscle and cartilage regeneration occurred in space animals as in synchronous controls. One of the most important differences between the two groups was the pigmentation of the blastemas: it was shown in laboratory, to be not due to a difference in light intensity.     

2 μm LIDAR for laser-based remote sensing: flight demonstrationand application survey

A flight test of a diode-pumped solid-state 2 μm Doppler Light Detection And Ranging (LIDAR) system was conducted on-board the NASA Ames DC-8 Airborne Laboratory. This was the first ever airborne demonstration of a 2 μm diode-pumped solid-state Doppler LIDAR. The LIDAR performance was verified by comparing the true-airspeed (TAS) estimate with that found using the pneumatic air data system; excellent agreement was found. The capabilities of this pulsed 2 μm Doppler LIDAR system include high bandwidth air data determination without the need for extensive forebody calibration, remote wind profiling as far as several kilometers away from the aircraft, eye-safe laser transmission at 2 μm, and diode-pumped solid-state design for compact construction and reliable performance     

Development of digital interactive processing system for NOAA satellites AVHRR data

The paper discusses the digital image processing system for NOAA/AVHRR data including Land applications — configured around VAX 11/750 host computer supported with FPS 100 Array Processor, Comtal graphic display and HP Plotting devices; wherein the system software for relational Data Base together with query and editing facilities, Man-Machine Interface using form, menu and prompt inputs including validation of user entries for data type and range; preprocessing software for data calibration, Sun-angle correction, Geometric Corrections for Earth curvature effect and Earth rotation offsets and Earth location of AVHRR image have been accomplished. The implemented image enhancement techniques such as grey level stretching, histogram equalization and convolution are discussed. The software implementation details for the computation of vegetative index and normalized vegetative index using NOAA/AVHRR channels 1 and 2 data together with output are presented; scientific background for such computations and obtainability of similar indices from Landsat/MSS data are also included. The paper concludes by specifying the further software developments planned and the progress envisaged in the field of vegetation index studies.