Biodosimetry of heavyions by interphase chromosome painting

We report measurements of chromosomal aberrations in peripheral blood lymphocytes from cancer patients undergoingradiotherapy treatment. Patients with cervix or esophageal cancer were treated with 10 MV X-rays produced at a LINAC accelerator, or high-energy carbon ions produced at the HIMAC accelerator at the National Institute for Radiological Sciences (NIRS) in Chiba. Blood samples were obtained before, during, and after the radiation treatment. Chromosomes were prematurely condensed by incubation in calyculin A. Aberrations in chromosomes 2 and 4 were scored after fluorescence in situ hybridization with whole-chromosome probes. Pre-treatment samples were exposed in vitro to X-rays, individual dose-response curves for the induction of chromosomal aberrations were determined, and used as calibration curves to calculate the effective whole-body dose absorbed during the treatment. This calculated dose, based on the calibration curve relative to the induction of reciprocal exchanges, has a sharp increase after the first few fractions of the treatment, then saturates at high doses. Although carbon ions are 2–3 times more effective than X-rays in tumor sterilization, the effective dose was similar to that of X-ray treatment. However, the frequency of complex-type chromosomal exchanges was much higher for patients treated with carbon ions than X-ray.     

Solar flare forecasting model supported with artificial neural network techniques

Nowadays operational models for solar activity forecasting are still based on the statistical relationship between solar activity and solar magnetic field evolution. In order to set up this relationship, many parameters have been proposed to be the measures. Conventional measures are based on the sunspot group classification which provides limited information from sunspots. For this reason, new measures based on solar magnetic field observations are proposed and a solar flare forecasting model supported with an artificial neural network is introduced. This model is equivalent to a person with a long period of solar flare forecasting experience.     

Simultaneous measurement of multiple radiation-induced protein expression profiles using the Luminex(TM) system.

Space flight results in the exposure of astronauts to a mixed field of radiation composed of energetic particles of varying energies, and biological indicators of space radiation exposure provides a better understanding of the associated long-term health risks. Current methods of biodosimetry have employed the use of cytogenetic analysis for biodosimetry, and more recently the advent of technological progression has led to advanced research in the use of genomic and proteomic expression profiling to simultaneously assess biomarkers of radiation exposure. We describe here the technical advantages of the Luminex(TM) 100 system relative to traditional methods and its potential as a tool to simultaneously profile multiple proteins induced by ionizing radiation. The development of such a bioassay would provide more relevant post-translational dynamics of stress response and will impart important implications in the advancement of space and other radiation contact monitoring.     

The radiation continuum model of light and the Galilean invarianceof Maxwell's equations

The original basis for the Lorentz transformations, and thus special relativity, was the assumption that the observed velocity of interaction of light with matter represents a unique velocity of the electromagnetic wave. This arbitrary decision is not borne out by Maxwell's theories or by any test that might prove that EM energy actually travels in a continuum of velocities. The second postulate as stated by Einstein does not deserve the status of a postulate, as it is at once overly restrictive and ultimately phenomenological-the nature of c is based on experimental measurement rather than on analysis of first principles. The radiation continuum model's (RCM) modified second postulate, however, says nothing about the actual propagation of EM energy, but only of the relative speed with which it must interact with matter to be detected. Utilizing this modified light principle we obtain an intuitive Galilean form invariance for Maxwell's equations. RCM places no upper limit on attainable velocities, and allows for the possibility of communications between humans or particles at speeds far in excess of c. This precludes many of the compatibility problems between the highly successful quantum mechanics and relativity theory     

Observations in the South Atlantic geomagnetic anomaly with intercosmos-Bulgaria-1300 during a geomagnetic storm

The region of South Atlantic Geomagnetic Anomaly (SAGA) was investigated by the Intercosmos-Bulgaria-1300 satellite, launched on August 7, 1981. On the basis of data obtained from 15 orbits during increased geomagnetic activity in August 1981, a map of the Anomaly was elaborated. Two centres of activity were identified. By means of the EMO-5 electrophotometer on board the Intercosmos-Bulgaria-1300 satellite, the atmosphere glow in lines λ 5577 Å, λ 6300 Å and λ 4278 Å was studied.     

On the structure and dynamics of the thermosphere

Thermospheric temperature, composition and wind measurements from the Dynamics Explorer satellite (DE-2) are interpreted using a three dimensional, multiconstituent spectral model. The analysis accounts for tides driven by the absorbed solar radiation as well as energy and momentum coupling involving the magnetosphere and lower atmosphere. We discuss phenomena associated with the annual tide, polar circulation, magnetic storms and substorms.     

The biological clock of Neurospora in a microgravity environment.

The circadian rhythm of conidiation in Neurospora crassa is thought to be an endogenously derived circadian oscillation; however, several investigators have suggested that circadian rhythms may, instead, be driven by some geophysical time cue(s). An experiment was conducted on space shuttle flight STS-9 in order to test this hypothesis; during the first 7-8 cycles in space, there were several minor alterations observed in the conidiation rhythm, including an increase in the period of the oscillation, an increase in the variability of the growth rate and a diminished rhythm amplitude, which eventually damped out in 25% of the flight tubes. On day seven of flight, the tubes were exposed to light while their growth fronts were marked. Some aspect of the marking process reinstated a robust rhythm in all the tubes which continued throughout the remainder of the flight. These results from the last 86 hours of flight demonstrated that the rhythm can persist in space. Since the aberrant rhythmicity occurred prior to the marking procedure, but not after, it was hypothesized that the damping on STS-9 may have resulted from the hypergravity pulse of launch. To test this hypothesis, we conducted investigations into the effects of altered gravitational forces on conidiation. Exposure to hypergravity (via centrifugation), simulated microgravity (via the use of a clinostat) and altered orientations (via alterations in the vector of a 1 g force) were used to examine the effects of gravity upon the circadian rhythm of conidiation.     

In situ measurements to determine if Phobos is layered

The complex system of linear features on Phobos—the inner moon of Mars—found by the Viking Orbiters in 1976, can be classified morphologically into three types according to their appearance as well as their geometrical distribution on the surface. One kind of grooves appears to form arcs of small circles normal to the Phobos-Mars direction. We propose that these grooves are the surface manifestation of layering within Phobos[4–6] which could have been formed when Phobos was a part of a much larger and geologically active parent planet.Here we suggest some measurements to observe whether Phobos has a layered structure or not. The suggestion contains remote sensing measurements (particularly determination of the magnetic field by magnetometer and by electron reflection method) by a spacecraft orbiting around Mars as well as surface measurements (active seismic reflection experiment) by a landing module on Phobos.     

Biological nitrogen fixation under primordial Martian partial pressures of dinitrogen.

Early Earth and early Mars were similar enough such that past geochemical and climatic conditions on Mars may have also been favorable for the origin of life. However, one of the most striking differences between the two planets was the low partial pressure of dinitrogen (pN2) on early Mars (18 mb). On Earth, nitrogen is a key biological element and in many ecosystems the low availability of fixed nitrogen compounds is the main factor limiting growth. Biological fixation of dinitrogen on Earth is a crucial source of fixed nitrogen. Could the low availability of dinitrogen in the primordial Martian atmosphere have prevented the existence, or evolution of Martian microbiota? Azotobacter vinelandii and Azomonas agilis were grown in nitrogen free synthetic medium under various partial pressures of dinitrogen ranging from 780-0 mb (total atmosphere=1 bar). Below 400 mb the biomass, cell number, and growth rate decreased with decreasing pN2. Both microorganisms were capable of growth at a pN2 as low as 5 mb, but no growth was observed at a pN2 < or = 1 mb. The data appear to indicate that biological nitrogen fixation could have occurred on primordial Mars (pN2=18 mb) making it possible for a biotic system to have played a role in the Martian nitrogen cycle. It is possible that nitrogen may have played a key role in the early evolution of life on Mars, and that later a lack of available nitrogen on that planet (currently, pN2=0.2 mb) may have been involved in its subsequent extinction.