Application of NASA's advanced life support technologies in polar regions.

NASA's advanced life support technologies are being combined with Arctic science and engineering knowledge in the Advanced Life Systems for Extreme Environments (ALSEE) project. This project addresses treatment and reduction of waste, purification and recycling of water, and production of food in remote communities of Alaska. The project focus is a major issue in the state of Alaska and other areas of the Circumpolar North; the health and welfare of people, their lives and the subsistence lifestyle in remote communities, care for the environment, and economic opportunity through technology transfer. The challenge is to implement the technologies in a manner compatible with the social and economic structures of native communities, the state, and the commercial sector. NASA goals are technology selection, system design and methods development of regenerative life support systems for planetary and Lunar bases and other space exploration missions. The ALSEE project will provide similar advanced technologies to address the multiple problems facing the remote communities of Alaska and provide an extreme environment testbed for future space applications. These technologies have never been assembled for this purpose. They offer an integrated approach to solving pressing problems in remote communities.     

Electrophoretic separation of kidney and pituitary cells on STS-8.

A Continuous Flow Electrophoresis System (CFES) was used on Space Shuttle flight STS-8 to separate specific secretory cells from suspensions of cultured primary human embryonic kidney cells and rat pituitary cells. The objectives were to isolate the subfractions of kidney cells that produce the largest amounts of urokinase (plasminogen activator), and to isolate the subfractions of rat pituitary cells that secrete growth hormone, prolactin, and other hormones. Kidney cells were separated into more than 32 fractions in each of two electrophoretic runs. Electrophoretic mobility distributions in flight experiments were spread more than the ground controls. Multiple assay methods confirmed that all cultured kidney cell fractions produced some urokinase, and five to six fractions produced significantly more urokinase than the other fractions. Several fractions also produced tissue plasminogen activator. The pituitary cells were separated into 48 fractions in each of the two electrophoretic runs, and the amounts of growth hormone (GH) and prolactin (PRL) released into the medium for each cell fraction were determined. Cell fractions were grouped into eight mobility classes and immunocytochemically assayed for the presence of GH, PRL, ACTH, LH, TSH, and FSH. The patterns of hormone distribution indicate that the specialized cells producing GH and PRL are isolatable due to the differences in electrophoretic mobilities.     

Degradation of cyanobacterial biosignatures by ionizing radiation

Primitive photosynthetic microorganisms, either dormant or dead, may remain today on the martian surface, akin to terrestrial cyanobacteria surviving endolithically in martian analog sites on Earth such as the Antarctic Dry Valleys and the Atacama Desert. Potential markers of martian photoautotrophs include the red edge of chlorophyll reflectance spectra or fluorescence emission from systems of light-harvesting pigments. Such biosignatures, however, would be modified and degraded by long-term exposure to ionizing radiation from the unshielded cosmic ray flux onto the martian surface. In this initial study into this issue, three analytical techniques--absorbance, reflectance, and fluorescence spectroscopy--were employed to determine the progression of the radiolytic destruction of cyanobacteria. The pattern of signal loss for chlorophyll reflection and fluorescence from several biomolecules is characterized and quantified after increasing exposures to ionizing gamma radiation. This allows estimation of the degradation rates of cyanobacterial biosignatures on the martian surface and the identification of promising detectable fluorescent break-down products.     

Dynamics of magnetospheric ion Composition as observed by the GEOS mass spectrometer

After one year of operation the GEOS-1 Ion Composition Experiment has surveyed plasma composition at all local times in the L range 3 8 and the energy per charge range from thermal to 16 keV/e. From measurements made in the keV range during eleven magnetic storms we find that the percentage of heavy (M/Q > 1) ions present in the outer magnetosphere increases by a factor of 3 to 10 during disturbances. We conclude that two independent sources (solar wind, characterized by ⁴He²⁺, and ionosphere, characterized by O⁺) give on the average comparable contributions to injected populations, although in a single event one or the other source may dominate. However, in magnetically quiet periods protons are the dominant species with a few percent of heavy ions. With the help of special satellite manoeuvres magnetic field aligned fluxes of 0.05-3 keV/e H⁺, He⁺, O⁺ with traces of O²⁺ have been observed which may be related to ion beams found previously at lower altitudes in the auroral zone. At still lower energies ( 1 eV/e) the thermal plasma population is found to be made up of six ion species, three of which, D⁺, He²⁺ and O²⁺, were unknown in the magnetosphere prior to the GEOS-1 measurements. We present here a study of the evolution of doubly charged ions and their parent populations over four consecutive days. Various production mechanisms for doubly charged ions are discussed. We argue that ionization of singly charged ions by UV and energetic electrons and protons is the dominant process for plasmasphere production. Furthermore, the observed high concentrations of O²⁺ at high altitudes are a result of production in the upper ionosphere and plasmasphere combined with upward transport by thermal diffusion. Throughout the 1 year lifetime of GEOS-1 the ICE functioned perfectly and, because of its novel design, a short review of technical performance is included here.     

Linear Frequency Modulation Derived Polyphase Pulse Compression Codes

Two new polyphase pulse compression codes and efficient digital implementation techniques are presented that are very Doppler tolerant and that can provide large pulse compression ratios. One of these codes is tolerant of precompression bandwidth limitations.