Microorganisms metabolizing on clay grains in 3-km-deep Greenland basal ice

We have discovered > 10(8) microbial cells/cm3 attached to clay grains in the bottom 13 m of the GISP2 (Greenland Ice Sheet Project) ice core. Their concentration correlates with huge excesses of CO2 and CH4. We show that Fe-reducing bacteria produce most of the excess CO2 and methanogenic archaea produce the excess CH4. The number of attached cells per clay grain is proportional to grain perimeter rather than to area, which implies that nutrients are accessed at grain edges. We conclude that Fe-reducing microbes immobilized on clay surfaces metabolize via "shuttle" molecules that transport electrons to grain edges, where they reduce Fe(III) ions at edges to Fe(II) while organic acid ions are oxidized to CO2. Driven by the concentration gradient, electrons on Fe(II) ions at grain edges "hop" to Fe(III) ions inward in the same edges and oxidize them. The original Fe(III) ions can then attach new electrons from shuttle molecules at the edges. Our mechanism explains how Fe-reducers can reduce essentially all Fe(III) in clay minerals. We estimate that the Fe(III) in clay grains in the GISP2 silty ice can sustain Fe-reducing bacteria at the ambient temperature of -9 degrees C for approximately 10(6) years. F420 autofluorescence imaging shows that > 2.4% of the cells are methanogens, which account for the excess methane.     

Proton Events and X-ray Flares in the Last Three Solar Cycles

A database joining the available information about proton enhancements near the Earth and their possible solar sources is organized on the basis of proton measurements of the GOES and IMP-8 satellites, the data of neutron monitors, and GOES X-ray measurements. One thousand one hundred and forty-four proton events with energy > 10 MeV have been selected in the period from 1975 to 2003. More than a half of these events can be reliably related to X-ray solar flares. A statistical analysis shows the probability of observing solar protons near the Earth and their maximum flux value to be strongly dependent on the importance of a flare and its heliolongitude. Proton events are recorded after all suitably located (western) flares with X-ray importance > X5. The heliolongitude of a flare predetermines the character of the time profile of proton events in many respects. The relationship of proton events with the other characteristics of flares is established. The flares associated with proton enhancements are characterized by longer duration, slower rise to the X-ray maximum, smaller temperature, and larger length of the X-ray loops.__________Translated from Kosmicheskie Issledovaniya, Vol. 43, No. 3, 2005, pp. 171–185.Original Russian Text Copyright © 2005 by Belov, Garcia, Kurt, Mavromichalaki.     

Application of GFP technique for cytoskeleton visualization onboard the International Space Station

Cytoskeleton recently attracted wide attention of cell and molecular biologists due to its crucial role in gravity sensing and trunsduction. Most of cytoskeletal research is conducted by the means of immunohistochemical reactions, different modifications of which are beneficial for the ground-based experiments. But for the performance onboard the space vehicles, they represent quite complicated technique which requires time and special skills for astronauts. In addition, immunocytochemistry provides only static images of the cytoskeleton arrangement in fixed cells while its localization in living cells is needed for the better understanding of cytoskeletal function. In this connection, we propose a new approach for cytoskeletal visualization onboard the ISS, namely, application of green fluorescent protein (GFP) from Aequorea victoria, which has the unique properties as a marker for protein localization in vivo. The creation of chimerical protein-GFP gene constructs, obtaining the transformed plant cells possessed protein-GFP in their cytoskeletal composition will allow receiving a simple and efficient model for screening of the cytoskeleton functional status in microgravity.     

The OSIRIS-REx Thermal Emission Spectrometer (OTES) Instrument

The OSIRIS-REx Thermal Emission Spectrometer (OTES) will provide remote measurements of mineralogy and thermophysical properties of Bennu to map its surface, help select the OSIRIS-REx sampling site, and investigate the Yarkovsky effect. OTES is a Fourier Transform spectrometer covering the spectral range 5.71–100 μm (\(1750\mbox{--}100~\mbox{cm}^{-1}\)) with a spectral sample interval of \(8.66~\mbox{cm}^{-1}\) and a 6.5-mrad field of view. The OTES telescope is a 15.2-cm diameter Cassegrain telescope that feeds a flat-plate Michelson moving mirror mounted on a linear voice-coil motor assembly. A single uncooled deuterated l-alanine doped triglycine sulfate (DLATGS) pyroelectric detector is used to sample the interferogram every two seconds. Redundant ～0.855 μm laser diodes are used in a metrology interferometer to provide precise moving mirror control and IR sampling at 772 Hz. The beamsplitter is a 38-mm diameter, 1-mm thick chemical vapor deposited diamond with an antireflection microstructure to minimize surface reflection. An internal calibration cone blackbody target provides radiometric calibration. The radiometric precision in a single spectrum is \(\leq2.2 \times 10^{-8}~\mbox{W}\,\mbox{cm}^{-2}\,\mbox{sr} ^{-1}/\mbox{cm}^{-1}\) between 300 and \(1350~\mbox{cm}^{-1}\). The absolute integrated radiance error is \(<1\%\) for scene temperatures ranging from 150 to 380 K. The overall OTES envelope size is \(37.5 \times 28.9 \times 52.2~\mbox{cm}\), and the mass is 6.27 kg. The power consumption is 10.8 W average. OTES was developed by Arizona State University with Moog Broad Reach developing the electronics. OTES was integrated, tested, and radiometrically calibrated on the Arizona State University campus in Tempe, AZ.     

The NASA Performance Assessment Workstation: cognitive performance during head-down bed rest

The NASA Performance Assessment Workstation was used to assess cognitive performance changes in eight males subjected to seventeen days of 6 degrees head-down bed rest. PAWS uses six performance tasks to assess directed and divided attention, spatial, mathematical, and memory skills, and tracking ability. Subjective scales assess overall fatigue and mood state. Subjects completed training trials, practice trials, bed rest trials, and recovery trials. The last eight practice trials and all bed rest trials were performed with subjects lying face-down on a gurney. In general, there was no apparent cumulative effect of bed rest. Following a short period of performance stabilization, a slight but steady trend of performance improvement was observed across all trials. For most tasks, this trend of performance improvement was enhanced during recovery. No statistically significant differences in performance were observed when comparing bed rest with the control period. Additionally, fatigue scores showed little change across all periods.     

Solar particle event dose distributions: parameterization of dose-time profiles

Calculations of total dose and dose equivalent as a function of time since the start of the event are presented for four of the major solar particle events that occurred during the period from August to December 1989. Results are presented for exposures to the skin, ocular lens and bone marrow shielded by a nominal thickness of aluminum shielding, comparable to that provided by a spacesuit. The calculated curves of organ dose and dose equivalent versus time are parameterized using a Weibull functional form for the fitting equation. The fitting parameters are determined using least squares regression techniques. These results provide a useful starting point for the development of methods to predict the cumulative doses and times to reach various dose limits from a limited number of dose measurements early in a solar particle event.     

Plasma Jet Motion Across the Geomagnetic Field in the “North Star” Active Geophysical Experiment

The active geophysical rocket experiment North Star was carried out in the auroral ionosphere on January 22, 1999, at the Poker Flat Research Range (Alaska, USA) using the American research rocket Black Brant XII with explosive plasma generators on board. Separable modules with scientific equipment were located at distances of from 170 to 1595 m from the plasma source. The experiment continued the series of the Russian–American joint experiments started by the Fluxus experiment in 1997. Two injections of aluminum plasma across the magnetic field were conducted in the North Star experiment. They were different, since in the first injection a neutral gas cloud was formed in order to increase the plasma ionization due to the interaction of neutrals of the jet and cloud. The first and second injections were conducted at heights of 360 and 280 km, respectively. The measurements have shown that the charged particle density was two orders of magnitude higher in the experiment with the gas release. The magnetic field in the first injection was completely expelled by the dense plasma of the jet. The displacement of the magnetic field in the second injection was negligible. The plasma jet velocity in both injections decreased gradually due to its interaction with the geomagnetic field. One of the most interesting results of the experiment was the conservation of high plasma density during the propagation of the divergent jet to considerable distances. This fact can be explained by the action of the critical ionization velocity mechanism.