Dynamics of Temperature and Density of Cold Protons of the Earth's Plasmasphere Measured by the Auroral Probe/Alpha-3 Experiment Data during Geomagnetic Disturbances

We present the results of temperature and density measurement of plasmaspheric protons under quiet and disturbed conditions in the night and dayside sectors of the plasmasphere obtained with the Auroral Probe/Alpha-3 instrument during September 1996 and January 1997. According to the experimental data, the proton temperature in the night sector of the plasmasphere depends on the level of geomagnetic disturbance: it is found that at night hours the values of temperatures inside the plasmasphere at 2.4 < L < 3.5 decreased considerably after the commencement of a geomagnetic storm. The temperature decrease, as a rule, was accompanied by the formation of a flat plateau on the density distribution n(L) at 2.4 < L < 3.5. The above experimental facts (decreasing proton temperature and formation of a flat part on the n(L) distribution) allow us to conclude that the decrease in the proton temperature in the night sector of the plasmasphere connected with magnetic disturbances is caused by the filling of field tubes (depleted after the commencement of the storm) with colder ionospheric plasma. The proton temperature in the dayside sector of the plasmasphere virtually does not depend on the level of the geomagnetic disturbance.     

Application of GFP technique for cytoskeleton visualization onboard the International Space Station

Cytoskeleton recently attracted wide attention of cell and molecular biologists due to its crucial role in gravity sensing and trunsduction. Most of cytoskeletal research is conducted by the means of immunohistochemical reactions, different modifications of which are beneficial for the ground-based experiments. But for the performance onboard the space vehicles, they represent quite complicated technique which requires time and special skills for astronauts. In addition, immunocytochemistry provides only static images of the cytoskeleton arrangement in fixed cells while its localization in living cells is needed for the better understanding of cytoskeletal function. In this connection, we propose a new approach for cytoskeletal visualization onboard the ISS, namely, application of green fluorescent protein (GFP) from Aequorea victoria, which has the unique properties as a marker for protein localization in vivo. The creation of chimerical protein-GFP gene constructs, obtaining the transformed plant cells possessed protein-GFP in their cytoskeletal composition will allow receiving a simple and efficient model for screening of the cytoskeleton functional status in microgravity.     

Cell-to-cell interactions in changed gravity: ground-based and flight experiments

Cell-to-cell interactions play an important role in all physiological processes and are mediated by humoral and mechanical factors. Mechanosensitive cells (e.g., osteocytes, chondrocytes, and fibroblasts) can be studied ex vivo to understand the effects of an altered gravity environment. In particular, cultured endothelial cells (EC) are very sensitive to a broad spectrum of mechanical and biochemical stimuli. Earlier, we demonstrated that clinorotation leads to cytoskeletal remodeling in cultured ECs. Long-term gravity vector changes also modulate the expression of surface adhesion molecules (ICAM-1, E-selectin, VCAM-1) on cultured ECs. To study the interactions of geterological cells, we cocultured endothelial monolayers and human lymphocytes, immune cells and myeloleucemic (K-560) cells. It was found that, although clinorotation did not alter the basal adhesion level of non-activated immune cells on endothelial monolayers, the adhesion of PMA-activated lymphocytes was increased. During flight experiments onboard the Russian segment of the International Space Station, we measured the cytotoxic activity of natural killer (NK) cells incubated with labeled target cells. It was found that immune cells in microgravity retained their ability to contact, recognize, and destroy oncogenic cells in vitro. Together, our data concerning the effects of simulated and real microgravity suggest that, despite changes in the cytoskeleton, cell motility, and expression of adhesion molecules, cell-cell interactions are not compromised, thus preserving the critical physiological functions of immune and endothelial cells.     

Exploring a structured decision approach as a means of fostering participatory space policy making at NASA

In 1996, the National Research Council's Committee on Risk Characterization argued convincingly for the implementation of more participatory approaches to improve policy making by incorporating a wide range of stakeholder values and concerns in policy decisions. Guidance about how to best carry out such an approach in an agency like NASA is less clear. To address this gap, this paper discusses how the use of a structured approach to involve expert and non-expert stakeholders in policy making can improve the quality of stakeholder involvement and resulting decisions for space policy making at NASA. Supporting this discussion are results from two recent experiments. One compared the quality and type of participants’ input in a conventional stakeholder workshop with that of a more structured participatory process. The results from this experiment showed that a structured decision approach leads to more thoughtful and better-informed decisions. A second experiment showed that structured, participatory decision processes can help to legitimize space policy decisions after they have been implemented, leading to future benefits for the space agency.     

UV photochemistry of DNA in vitro and in Bacillus subtilis spores at earth-ambient and low atmospheric pressure: implications for spore survival on other planets or moons in the solar system

Two major parameters influencing the survival of Bacillus subtilis spores in space and on bodies within the Solar System are UV radiation and vacuum, both of which induce inactivating damage to DNA. To date, however, spore survival and DNA photochemistry have been explored only at the extremes of Earth-normal atmospheric pressure (101.3 kPa) and at simulated space vacuum (10(-3)-10(-6) Pa). In this study, wild-type spores, mutant spores lacking alpha/beta-type small, acid-soluble spore proteins (SASP), naked DNA, and complexes between SASP SspC and DNA were exposed simultaneously to UV (254 nm) at intermediate pressure (1-2 Pa), and the UV photoproducts cis,syn-thymine-thymine cyclobutane dimer (c,sTT), trans,syn-thymine-thymine cyclobutane dimer (t,sTT), and "spore photoproduct" (SP) were quantified. At 101.3 kPa, UV-treated wild-type spores accumulated only SP, but spores treated with UV radiation at 1-2 Pa exhibited a spectrum of DNA damage similar to that of spores treated at 10(-6) Pa, with accumulation of SP, c,sTT, and t,sTT. The presence or absence of alpha/beta-type SASP in spores was partly responsible for the shift observed between levels of SP and c,sTT, but not t,sTT. The changes observed in spore DNA photochemistry at 1-2 Pa in vivo were not reproduced by irradiation of naked DNA or SspC:DNA complexes in vitro, suggesting that factors other than SASP are involved in spore DNA photochemistry at low pressure.     

Field emission performance of multiwalled carbon nanotubes for a low-power spacecraft neutraliser

Field electron emission from aligned multiwalled carbon nanotubes has been assessed to determine if the performance, defined by power consumption, lifetime and emission current, is suitable for use in spacecraft charge neutralisation for field emission electric propulsion (FEEP). Carbon nanotubes grown by chemical vapour deposition (CVD) were mounted on a dual in line chip with a macroscopic (nickel mesh) extractor electrode mounted ～1 mm above the tubes. The nanotubes’ field emission characteristics (emission currents, electron losses and operating voltage) were measured at ～10^?4 Pa. An endurance test of one sample, running at a software-controlled constant emission current lasted >1400 h, approaching the longest known FEEP thruster lifetime. The emission corresponds to a current density of ～10 mA/cm² at a voltage of 150 V. These results, implementing mature extractor-electrode geometry, indicate that carbon nanotubes have considerable potential for development as robust, low-power, long-lived electron emitters for use in space.     

Power law distribution in statistics of failures in operation of spacecraft onboard equipment

The possibility of using the statistics of recurrence time for extreme events is studied in this paper having in mind the problems of control and prediction of failures in spacecraft operation. The information about failures onboard satellites of various types presented by the US National Geophysical Data Center was analyzed. It was found that the probability density of recurrence intervals followed a power law of the Pareto type with an index equal to 2.3. The obtained result is consistent both with the theory of normal catastrophes and with the principle of self-organization of criticality for metastable active heterogeneous environment. A practical consequence of the obtained result consists in the fact that predictions of these extreme events should not rely on traditional models with the second-order Pearson statistics. To make predictions, the models are necessary that take into account the power law distribution of recurrence intervals for failures on satellites. The failures should be considered in these models as extreme events connected with manifestation of the space environment factors.     

Construction of theories of motion, ephemerides, and databases for natural satellites of planets

A knowledge base for natural satellites of planets is created. On the basis of observations, new numerical models of motion are constructed for all 96 outer satellites of Jupiter, Saturn, Uranus, and Neptune. A special database is compiled from all available observational data for natural satellites of planets, as well as a bibliographical database and information system of physical and orbital parameters of planets and satellites. The tools for calculations of ephemerides of all natural satellites (except for the Moon) of planets are developed. They represent the final result of studies and actually incorporate the entire knowledge about dynamics of the satellites of planets. Also developed are special ephemerides in order to observe singular phenomena in the apparent motion of the satellites of planets. A computer environment and the Internet allowed us to concentrate all above-listed options in a single toolkit easily available to any user in the world.     

Survival and germinability of Bacillus subtilis spores exposed to simulated Mars solar radiation: implications for life detection and planetary protection

Bacterial spores have been considered as microbial life that could survive interplanetary transport by natural impact processes or human spaceflight activity. Deposition of terrestrial microbes or their biosignature molecules onto the surface of Mars could negatively impact life detection experiments and planetary protection measures. Simulated Mars solar radiation, particularly the ultraviolet component, has been shown to reduce spore viability, but its effect on spore germination and resulting production of biosignature molecules has not been explored. We examined the survival and germinability of Bacillus subtilis spores exposed to simulated martian conditions that include solar radiation. Spores of B. subtilis that contain luciferase resulting from expression of an sspB-luxAB gene fusion were deposited on aluminum coupons to simulate deposition on spacecraft surfaces and exposed to simulated Mars atmosphere and solar radiation. The equivalent of 42 min of simulated Mars solar radiation exposure reduced spore viability by nearly 3 logs, while germination-induced bioluminescence, a measure of germination metabolism, was reduced by less than 1 log. The data indicate that spores can retain the potential to initiate germination-associated metabolic processes and produce biological signature molecules after being rendered nonviable by exposure to Mars solar radiation.     

An examination of the carbon isotope effects associated with amino acid biosynthesis

Stable carbon isotope ratios (delta(13)C) were determined for alanine, proline, phenylalanine, valine, leucine, isoleucine, aspartate (aspartic acid and asparagine), glutamate (glutamic acid and glutamine), lysine, serine, glycine, and threonine from metabolically diverse microorganisms. The microorganisms examined included fermenting bacteria, organotrophic, chemolithotrophic, phototrophic, methylotrophic, methanogenic, acetogenic, acetotrophic, and naturally occurring cryptoendolithic communities from the Dry Valleys of Antarctica. Here we demonstrated that reactions involved in amino acid biosynthesis can be used to distinguish amino acids formed by life from those formed by nonbiological processes. The unique patterns of delta(13)C imprinted by life on amino acids produced a biological bias. We also showed that, by applying discriminant function analysis to the delta(13)C value of a pool of amino acids formed by biological activity, it was possible to identify key aspects of intermediary carbon metabolism in the microbial world. In fact, microorganisms examined in this study could be placed within one of three metabolic groups: (1) heterotrophs that grow by oxidizing compounds containing three or more carbon-to-carbon bonds (fermenters and organotrophs), (2) autotrophs that grow by taking up carbon dioxide (chemolitotrophs and phototrophs), and (3) acetoclastic microbes that grow by assimilation of formaldehyde or acetate (methylotrophs, methanogens, acetogens, and acetotrophs). Furthermore, we demonstrated that cryptoendolithic communities from Antarctica grouped most closely with the autotrophs, which indicates that the dominant metabolic pathways in these communities are likely those utilized for CO(2 )fixation. We propose that this technique can be used to determine the dominant metabolic types in a community and reveal the overall flow of carbon in a complex ecosystem.