Genomic instability and tumorigenic induction in immortalized human bronchial epithelial cells by heavy ions

Carcinogenesis is postulated to be a progressive multistage process characterized by an increase in genomic instability and clonal selection with each mutational event endowing a selective growth advantage. Genomic instability as manifested by the amplification of specific gene fragments is common among tumor and transformed cells. In the present study, immortalized human bronchial (BEP2D) cells were irradiated with graded doses of either 1GeV/nucleon ⁵⁶Fe ions or 150 keV/μm alpha particles. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Tumorigenic cells showed neither ras mutations nor deletion in the p16 tumor suppressor gene. In contrast, they harbored mutations in the p53 gene and over-expressed cyclin D1. Genomic instability among transformed cells at various stage of the carcinogenic process was examined based on frequencies of PALA resistance. Incidence of genomic instability was highest among established tumor cell lines relative to transformed, non-tumorigenic and control cell lines. Treatment of BEP2D cells with a 4 mM dose of the aminothiol WR-1065 significantly reduced their neoplastic transforming response to ⁵⁶Fe particles. This model provides an opportunity to study the cellular and molecular mechanisms involved in malignant transformation of human epithelial cells by heavy ions.     

On the structure and dynamics of the thermosphere

Thermospheric temperature, composition and wind measurements from the Dynamics Explorer satellite (DE-2) are interpreted using a three dimensional, multiconstituent spectral model. The analysis accounts for tides driven by the absorbed solar radiation as well as energy and momentum coupling involving the magnetosphere and lower atmosphere. We discuss phenomena associated with the annual tide, polar circulation, magnetic storms and substorms.     

Biological nitrogen fixation under primordial Martian partial pressures of dinitrogen.

Early Earth and early Mars were similar enough such that past geochemical and climatic conditions on Mars may have also been favorable for the origin of life. However, one of the most striking differences between the two planets was the low partial pressure of dinitrogen (pN2) on early Mars (18 mb). On Earth, nitrogen is a key biological element and in many ecosystems the low availability of fixed nitrogen compounds is the main factor limiting growth. Biological fixation of dinitrogen on Earth is a crucial source of fixed nitrogen. Could the low availability of dinitrogen in the primordial Martian atmosphere have prevented the existence, or evolution of Martian microbiota? Azotobacter vinelandii and Azomonas agilis were grown in nitrogen free synthetic medium under various partial pressures of dinitrogen ranging from 780-0 mb (total atmosphere=1 bar). Below 400 mb the biomass, cell number, and growth rate decreased with decreasing pN2. Both microorganisms were capable of growth at a pN2 as low as 5 mb, but no growth was observed at a pN2 < or = 1 mb. The data appear to indicate that biological nitrogen fixation could have occurred on primordial Mars (pN2=18 mb) making it possible for a biotic system to have played a role in the Martian nitrogen cycle. It is possible that nitrogen may have played a key role in the early evolution of life on Mars, and that later a lack of available nitrogen on that planet (currently, pN2=0.2 mb) may have been involved in its subsequent extinction.     

Current status and future plans for the general antiparticle spectrometer (GAPS)

We discuss current progress and future plans for the general antiparticle spectrometer experiment (GAPS). GAPS detects antideuterons through the X-rays and pions emitted during the deexcitation of exotic atoms formed when the antideuterons are slowed down and stopped in targets. GAPS provides an exceptionally sensitive means to detect cosmic-ray antideuterons. Cosmic-ray antideuterons can provide indirect evidence for the existence of dark matter in such form as neutralinos or Kaluza–Klein particles. We describe results of accelerator testing of GAPS prototypes, tentative design concepts for a flight GAPS detector, and near-term plans for flying a GAPS prototype on a balloon.     

Temperature comparison between CHAMP radio occultation and TIMED/SABER measurements in the lower stratosphere

Global Positioning System (GPS) receiver on the CHAllenging Mini-satellite Payload (CHAMP) and the Sounding of the Atmosphere using Broadband Emission Radiometry (SABER) instrument, one of four on board the TIMED satellite, provide middle atmosphere temperature profiles by Radio Occultation (RO) and limb viewing infrared emission measurements, respectively. These temperature profiles retrieved by two different techniques in the stratosphere are compared with each other using more than 1300 correlative profiles in March, September and December 2005. The over-all mean differences averaged over 15 and 35 km are approximately −2 K and standard deviation is less than 3 K. Below 20 km of altitude, relatively small mean temperature differences ∼1 K are observed in wide latitudinal range except for June (during the SABER nighttime observation). In the middle to low latitudes, between 30°S and 30°N, the temperature difference increases with height from ∼0–1 K at 15 km, to ∼−4 K at 35 km of altitude. Large temperature differences about −4 to −6 K are observed between 60°S and 30°N and 31–35 km of altitude for all months and between 0° and 30°N below 16 km during June (nighttime).     

Fourier Analysis of Commutated-Capacitor-Type Integrator Output Signal

It is shown that an operational amplifier with four commutated capacitors in the feedback path produces an output signal whose fundamental is equal in magnitude but 180 degrees out of phase with the input. The analysis is carried out by expressing the output signal in a Fourier series.     

Evaluation of SPOT HRV simulation data for Corps of Engineers applications

During the summer of 1983 three Corps of Engineers project sites were overflown as part of the SPOT (Système Probatoire d'Observation de la Terre) High Resolution Visible (HRV) simulation campaign. The three sites were Chesapeake Bay, Maryland, Berlin Lake, Ohio, and Lac qui Parle, Minnesota. Multispectral imagery data at a 20-m resolution for three spectral bands (0.50–0.59 μm, 0.61–0.68 μm, 0.79–0.89 μm) were obtained for each of the sites. The data were analyzed for use in dredging, recreation resource management, water quality, and wildlife habitat applications.     

Development of an improved ground-based prototype of space plant-growing facility

Based on a formerly developed ground-based prototype of space plant-growing facility, the development of its improved prototype has been finished, so as to make its operating principle better adapt to the space microgravity environment. According to the developing experience of its first generation prototype and detailed demonstration and design of technique plan, its blueprint design and machining of related components, whole facility installment, debugging and trial operations were all done gradually. Its growing chamber contains a volume of about 0.5 m³ and a growing area of approximate 0.5 m²; the atmospheric environmental parameters in the growing chamber and water content in the growing media were controlled totally and effectively; lighting source is a combination of both red and blue light emitting diodes (LED). The following demonstrating results showed that the entire system design of the prototype is reasonable and its operating principle can nearly meet the requirements of space microgravity environment. Therefore, our plant-growing technique in space was advanced further, which laid an important foundation for next development of the space plant-growing facility and plant-cultivating experimental research in space microgravity condition.     

Neutral Upper Atmosphere and Ionosphere Modeling

Numerical modeling tools can be used for a number of reasons yielding many benefits in their application to planetary upper atmosphere and ionosphere environments. These tools are commonly used to predict upper atmosphere and ionosphere characteristics and to interpret measurements once they are obtained. Additional applications of these tools include conducting diagnostic balance studies, converting raw measurements into useful physical parameters, and comparing features and processes of different planetary atmospheres. This chapter focuses upon various classes of upper atmosphere and ionosphere numerical modeling tools, the equations solved and key assumptions made, specified inputs and tunable parameters, their common applications, and finally their notable strengths and weaknesses. Examples of these model classes and their specific applications to individual planetary environments will be described.