Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms.

It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (AprLux+) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g l-1) taken from Lake Shira (Khakasia, Russia). The survival of the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor-ampicillin (50 micrograms/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The populations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 micrograms/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 micrograms/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retails the recombinant plasmid.     

藜蒿中绿原酸溶剂提取方法研究

应用醇溶剂回流法提取藜蒿中绿原酸，通过正交试验，对藜蒿中绿原酸进行溶剂提取条件的优化研究，并分剐讨论了各因素对绿原酸提取率的影响。实验表明，乙醇浓度70％、12倍的提取剂用量、温度85℃、提取时间4h、提取两次为乙醇溶剂提取藜蒿中绿原酸的最佳条件，总绿原酸提取率为1.24％。     

PC-index fluctuations and intermittency of the magnetospheric dynamics

One minute resolution Polar Cap (PC) index was used for the analysis of magnetospheric dynamics. The 1995–2000 time series analysis revealed that the power spectrum of the PC-index fluctuations is a power law in a wide range of frequencies. However, the obtained exponents differ for low and high frequency regions. The probability distribution functions of the PC-index fluctuations show a strong non-gaussian shape, depending on the time of increment. This indicates that the PC-index exhibits intermittency, previously detected in solar wind and auroral electrojet index fluctuations. The PC-index probability distribution functions were fitted by the functional form proposed by Castaing et al. [Velocity probability density functions of high Reynolds number turbulence. Physica D. 46, 177–200, 1990] to describe intermittency phenomena in ordinary turbulent fluid flows. The agreement between the fitting parameters obtained for the PC index and those reported before for solar wind magnetic field fluctuations is within 30%; which is noticeably less than the difference between the same parameters of solar wind and the AE-index fluctuations. This fact indicates that the PC index reflects the solar wind influence on the high-latitude magnetosphere, especially during the summer.     

Top-level modeling of an ALS system utilizing object-oriented techniques.

The possible configuration of an Advanced Life Support (ALS) System capable of supporting human life for long-term space missions continues to evolve as researchers investigate potential technologies and configurations. To facilitate the decision process the development of acceptable, flexible, and dynamic mathematical computer modeling tools capable of system level analysis is desirable. Object-oriented techniques have been adopted to develop a dynamic top-level model of an ALS system.This approach has several advantages; among these, object-oriented abstractions of systems are inherently modular in architecture. Thus, models can initially be somewhat simplistic, while allowing for adjustments and improvements. In addition, by coding the model in Java, the model can be implemented via the World Wide Web, greatly encouraging the utilization of the model. Systems analysis is further enabled with the utilization of a readily available backend database containing information supporting the model. The subsystem models of the ALS system model include Crew, Biomass Production, Waste Processing and Resource Recovery, Food Processing and Nutrition, and the Interconnecting Space. Each subsystem model and an overall model have been developed. Presented here is the procedure utilized to develop the modeling tool, the vision of the modeling tool, and the current focus for each of the subsystem models.     

Escaping near-relativistic electron beams from the solar corona

Processes in the solar corona are prodigious accelerators of energetic ions, and electrons. The angular distribution, composition, and spectra of energetic particles observed near Earth gives information on the acceleration mechanisms. A class of energetic particle observations particularly useful in understanding the solar acceleration is the near-relativistic impulsive beam-like electron events. During five years of operation the Advanced Composition Explorer (ACE) has measured well over 400 electron events. Approximately 25% of these electron events are impulsive beam-like events that are released onto interplanetary field lines predominantly from western solar longitudes. We extend our initial 3 year study during the rise to solar maximum (Haggerty and Roelof, 2002; Simnett et al., 2002) to a five year statistical analysis of these beam-like energetic electron events in relationship to optical flares, microwave emission, soft X-ray emission, metric and decametric type-III radio bursts, and coronal mass ejections.     

Microgravity particle research on the space station: the Gas-Grain Simulation Facility.

In the gravitational field on Earth, the large settling rate of micron-sized particles and the effects of gravity-induced convection prohibit many interesting studies of phenomena such as coagulation, collisions, and mutual interactions of droplets, dust grains and other particles. Examples of exobiology experiments involving these phenomena are the simulation of organic aerosol formation in Titan's atmosphere, studies of the role of comets in prebiotic chemical evolution, and simulations of carbon grain interactions in various astrophysical environments. The Gas-Grain Simulation Facility (GGSF) is a proposed Earth-orbital laboratory that will allow present ground-based experimental programs which study processes involving small particles and weak interactions to be extended to a new domain. Physics issues that scientists wishing to propose GGSF experiments must consider are reviewed in this paper. Specifically, coagulation, motion in gases and vacua, and wall deposition of particles in a microgravity environment are discussed.     

Spending without results: lessons from the space station program

The US spent all of the funds originally estimated for the initial development of its orbital space station without producing any significant amount of flight hardware. This article shows how a project with large design costs and significant “non-prime” outlays can quickly deplete program funds. The authors recount the way in which budgetary politics, congressional micro-management, and technological risk conspired to produce this result.     

Mobile Satellite Plans and Status

A variety of new communication functions will become available near the end of the decade when mobile satellite services (MSS) are introduced commercially in the United States and Canada. Mobile radios, hand carried radios and small fixed transponders will communicate directly through satellites into the telephone network or to private base stations. Voice, data, and position fixing services will be distance insensitive with good performance in difficult terrain and remote locations. Experiments with NASA satellites and studies in the U.S. and Canada demonstrated the technical feasibility and practical applications of mobile satellites. In 1983 Mobile Satellite Corporation, followed by Skylink Corporation, applied to the FCC for authorization to build and operate a land and aeronautical mobile satellite system for the United States. Canada had advanced its plans for an MSAT system. In response to these initiatives, the FCC issued a ``Notice of Proposed Rulemaking' that proposed the allocation of radio spectrum for the new service and asked for applications from prospective providers. Twelve applications were received. The shortage of radio spectrum in the U.S. has resulted in much contention over the proposed allocations, and the numerous applications have introduced competing systems concepts. The paper describes a likely system implementation, its services and markets, and the current regulatory status.     

An approach to knowledge-aided covariance estimation

This paper introduces a parametric covariance estimation scheme for use with space-time adaptive processing (STAP) methods operating in heterogeneous clutter environments. The approach blends both a priori knowledge and data observations within a parameterized model to capture instantaneous characteristics of the cell under test (CUT) and reduce covariance errors leading to detection performance loss. We justify this method using both measured and synthetic data. Performance potential for the specific operating conditions examined herein include: 1) averaged behavior within roughly 2 dB of the optimal filter, 2) 1 dB improvement in exceedance characteristic relative to the optimal filter, highlighting improved instantaneous capability, and 3) impervious ness to corruptive target-like signals in the secondary data (no additional signal-to-interference-plus-noise ratio (SINK) loss, compared with 10 dB or greater loss for the standard STAP implementation), with corresponding detections comparable to the optimal filter case     

A root moisture sensor for plants in microgravity.

Development of components for bioregenerative life-support systems is a vital step toward long-term space exploration. The culturing of plants in a microgravity environment may be optimized by the use of appropriate sensors and controllers. This paper describes a sensor developed for determining the amount of fluid (nutrient solution) available on the surface of a porous ceramic nutrient delivery substrate to the roots of conventional crop plants. The sensor is based on the change in thermal capacitance and thermal conductance near the surface as the moisture content changes. The sensor could be employed as a data acquisition and control sensor to support the automated monitoring of plants grown in a microgravity environment.