Extreme dryness and DNA-protein cross-links.

Exposure of fungal conidia (Aspergillus ochraceus) or spores of Bacillus subtilis to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered water activity results in an increasing number of cross-links between DNA and proteins. In fungal conidia these cross-links are detected after selective iodination (125 J) of the DNA-bound proteins followed by gel electrophoresis and subsequent autoradiography. Another approach is the labelling of DNA with 32P by means of nick translation and the detection of differences in the electrophoretic mobility of DNA before and after digestion with proteinase K of proteins bound to DNA.     

Infrared spectroscopy of organics of planetological interest at low temperatures.

In the context of prebiotic chemistry in space, some of the outer planetary objects display H, C, N and O rich chemistry similar to the one in the biosphere of Earth. Of particular interest are Saturn's moon, Titan; Neptune's moon, Triton; and Pluto where extreme cold conditions prevail. Identifications of chemical species on these objects (surfaces and atmospheres) is essential to a better understanding of the radiation induced chemical reactions occurring thereon. There have been several ground based observations of these planetary objects in the infrared windows from 1 to 2.5 micrometers. Voyager also provided spectra in the thermal infrared (6 to 50 micrometers) region. Interpretation of these data require laboratory infrared spectra of relevant species under the temperature conditions appropriate to these objects. The results of some of these studies carried out in our laboratory and elsewhere and their impact on the analyses of the observed data will be summarized.     

Reduced lymphocyte activation in space: role of cell-substratum interactions.

We investigated the effect of substratum adhesiveness on stimulated lymphocyte blastogenesis by reducing and blocking cell adhesion with poly (2-hydroxyethyl methacrylate) (poly-HEMA) in a simple on-ground system. Cells grown on medium-thick and thick poly-HEMA films were rounded in shape and displayed no signs of spreading. By contrast, on tissue culture plastic and very thin poly-HEMA films, they showed clear signs of spreading. The mitogenic response of lymphocytes grown on thick poly-HEMA films was reduced by up to 68% of the control (tissue culture plastic). Interferon-gamma production was near zero when the cells were grown on the least adhesive substratum. On uncoated plastic, activated lymphocytes subjected to high gravity (20g) exhibited an increased proliferation rate (40%) compared with 1g. By contrast, on poly-HEMA, high gravity did not improve lymphocyte responsiveness. These results show that activated lymphocytes need to anchor and spread prior to achieving an optimal proliferation response. We conclude that decreased lymphocyte adhesion could contribute to the depressed in vitro lymphocyte responsiveness found in the microgravity conditions of space flight.     

Several recent findings concerning the dynamics of the Earth's magnetotail

Several recent results concerning the nature of the Earth's magnetotail are briefly reviewed. These observational findings include: (1) the three-dimensional character of the plasma sheet via a comprehensive survey of proton bulk flows, (2) a region of earthward flowing plasmas at the interfaces of the plasma sheet and magnetotail lobes during magnetic substorm recovery, and (3) the signature of electrostatic acceleration for protons within the jetting plasmas from magnetotail fireballs.Proceedings of the Symposium on Solar Terrestrial Physics held in Innsbruck, May–June 1978.     

HIRDES – The High-Resolution Double-Echelle Spectrograph for the World Space Observatory Ultraviolet (WSO/UV)

The World Space Observatory Ultraviolet (WSO/UV) is a multi-national project grown out of the needs of the astronomical community to have future access to the UV range. WSO/UV consists of a single UV telescope with a primary mirror of 1.7 m diameter feeding the UV spectrometer and UV imagers. The spectrometer comprises three different spectrographs, two high-resolution echelle spectrographs (the High-Resolution Double-Echelle Spectrograph, HIRDES) and a low-dispersion long-slit instrument. Within HIRDES the 102–310 nm spectral band is split to feed two echelle spectrographs covering the UV range 174–310 nm and the vacuum-UV range 102–176 nm with high spectral resolution (R > 50,000). The technical concept is based on the heritage of two previous ORFEUS SPAS missions. The phase-B1 development activities are described in this paper considering performance aspects, design drivers, related trade-offs (mechanical concepts, material selection etc.) and a critical functional and environmental test verification approach. The current state of other WSO/UV scientific instruments (imagers) is also described.     

Investigation of vertical profile of rain microstructure at Ahmedabad in Indian tropical region

The microstructure of rain has been studied with observations using a vertical looking Micro Rain Radar (MRR) at Ahmedabad (23.06°N, 72.62°E), a tropical location in the Indian region. The rain height, derived from the bright band signature of melting layer of radar reflectivity profile, is found to be variable between the heights 4600 m and 5200 m. The change in the nature of rain, classified on the basis of radar reflectivity, is also observed through the MRR. It has been found that there are three types of rain, namely, convective, mixed and stratiform rain, prevailing with different vertical rain microstructures, such as, Drop Size Distribution (DSD), mean drop size, rain rate, liquid water content and average fall speed of the drops at different heights. It is observed that the vertical DSD profile is more inhomogeneous for mixed and stratiform type rain than for convective type rain. It is also found that the large number of drops of size <0.5 mm is present in convective rain whereas in stratiform rain, drops concentration is appreciable up to 1 mm. A comparison of measurements taken by ground based Disdrometer and that from the 200 m level obtained from MRR shows good agreement for rain rate and DSD at smaller rain rate values. The results may be useful for understanding rain structures over this region.     

Energy dependence of the characteristic decay time of proton fluxes in solar cosmic ray events

Energetic solar proton events within the energy interval 1–48 MeV at the stage of their decay are considered over the period of 1974–2001. The dependence of the characteristic decay time on the proton energy in the assumed power-law representation τ(E) =E ^?n is analyzed for the events with an exponential decay form. The dependence of n on the heliolongitude of the flare (the particles source on the Sun) is studied.     

Micro- and mini-satellites of ISRO—technology and applications

With rich experience of the successful Indian remote sensing satellite series, Indian Space Research Organization (ISRO) has started theme-based satellites like Resourcesat and Oceansat. Further taking the advantage of the improved technologies in areas of miniaturization, the micro- and mini-satellite series have been started, which will provide opportunity for the payloads of stand-alone missions, for applications, study or research. These include payloads for Earth imaging, atmospheric monitoring, ocean monitoring, scientific applications, and stellar observation. The micro-satellites are of 100 kg class, planned with a payload of about 30 kg and 20 W power and mini-satellites of 450 kg class for payloads of 200 kg and power of 200 W. The first satellite in the micro-satellite series is an Earth imaging payload followed by the second satellite with scientific payloads with the participation of students. Further the scientific proposals for micro-satellites are under evaluation. Similarly the first two missions of mini-satellites are defined with first one carrying ocean and environment monitoring payloads followed by the Earth imaging satellite with multi-spectral camera with 700 km swath. The current paper touches upon the technology involved in realization of the micro- and mini-satellites and the scope of applications of the series.     

Mutual coupling effects and their reduction in wideband directionof arrival estimation

The performance of multiple signal classification (MUSIC) algorithm using three different inputs over a wideband of frequencies is considered. These inputs are: 1) ideal voltages, 2) actual voltages which include coupling effects and are obtained with the method or moments, and 3) corrected voltages which are obtained from the actual voltages so that the mutual coupling effects are removed. Linear arrays of dipoles, sleeve dipoles, and spiral antennas are considered over 200 MHz to 400 MHz band