Composition and physical properties of starch in microgravity-grown plants.

The effect of spaceflight on starch development in soybean (Glycine max L., BRIC-03) and potato (Solanum tuberosum, Astroculture-05) was compared with ground controls by biophysical and biochemical measurements. Starch grains from plants from both flights were on average 20-50% smaller in diameter than ground controls. The ratio delta X/delta rho (delta X --difference of magnetic susceptibilities, delta rho--difference of densities between starch and water) of starch grains was ca. 15% and 4% higher for space-grown soybean cotyledons and potato tubers, respectively, than in corresponding ground controls. Since the densities of particles were similar for all samples (1.36 to 1.38 g/cm3), the observed difference in delta X/delta rho was due to different magnetic susceptibilities and indicates modified composition of starch grains. In starch preparations from soybean cotyledons (BRIC-03) subjected to controlled enzymatic degradation with alpha-amylase for 24 hours, 77 +/- 6% of the starch from the flight cotyledons was degraded compared to 58 +/- 12% in ground controls. The amylose content in starch was also higher in space-grown tissues. The good correlation between the amylose content and delta X/delta rho suggests, that the magnetic susceptibility of starch grains is related to their amylose content. Since the seedlings from the BRIC-03 experiment showed elevated post-flight ethylene levels, material from another flight experiment (GENEX) which had normal levels of ethylene was examined and showed no difference to ground controls in size distribution, density, delta X/delta rho and amylose content. Therefore the role of ethylene appears to be more important for changes in starch metabolism than microgravity.     

First radiobiological results of LDEF-1 experiment A0015 with Arabidopsis seed embryos and Sordaria fungus spores.

This article highlights the first results of investigations on the general vitality and damage endpoints caused by cosmic ionizing radiation in dry, dormant plant seeds of the crucifer plant Arabidopsis thaliana (L.) Heynh. and the ascomycete Sordaria fimicola after 69 month stay in space. Wild-type and mutant gene marker lines were included in Free Flyer Biostack containers and exposed on earth and side tray of the LDEF-1 satellite. The damage in biological endpoints observed in the seeds increased in the side tray sample compared to the earth tray sample. For the ascospores we found different effects depending on the biological endpoints investigated for both expositions.     

Particle trajectories in seeds of Lactuca sativa and chromosome aberrations after exposure to cosmic heavy ions on Cosmos Biosatellites 8 and 9.

The potentially specific importance of the heavy ions of the galactic cosmic radiation for radiation protection in manned spaceflight continues to stimulate in situ, i.e., spaceflight experiments to investigate their radiobiological properties. Chromosome aberrations as an expression of a direct assault on the genome are of particular interest in view of cancerogenesis being the primary radiation risk for man in space. In such investigations the establishment of the geometrical correlation between heavy ions' trajectories and the location of radiation sensitive biological substructures is an essential task. The overall qualitative and quantitative precision achieved for the identification of particle trajectories in the order of approximately 10 micrometers as well as the contributing sources of uncertainties are discussed. We describe how this was achieved for seeds of Lactuca sativa as biological test organisms, whose location and orientation had to be derived from contact photographies displaying their outlines and those of the holder plates only. The incidence of chromosome aberrations in cells exposed during the COSMOS 1887 (Biosatellite 8) and the COSMOS 2044 (Biosatellite 9) mission was determined for seeds hit by cosmic heavy ions. In those seeds the incidence of both single and multiple chromosome aberrations was enhanced. The results of the Biosatellite 9 experiment, however, are confounded by spaceflight effects unrelated to the passage of heavy ions.     

Formation of bioorganic compounds in simulated planetary atmospheres by high energy particles or photons.

Various types of organic compounds have been detected in Jupiter, Titan, and cometary coma. It is probable that organic compounds were formed in primitive Earth and Mars atmospheres. Cosmic rays and solar UV are believed to be two major energy sources for organic formation in space. We examined energetics of organic formation in simulated planetary atmospheres. Gas mixtures including a C-source (carbon monoxide or methane) and a N-source (nitrogen or ammonia) was irradiated with the followings: High energy protons or electrons from accelerators, gamma-rays from 60Co, UV light from a deuterium lamp, and soft X-rays or UV light from an electron synchrotron. Amino acids were detected in the products of particles, gamma-rays and soft X-rays irradiation from each gas mixture examined. UV light gave, however, no amino acid precursors in the gas mixture of carbon monoxide, nitrogen and nitrogen. It gave only a trace of them in the gas mixture of carbon monoxide, ammonia and water or that of methane, nitrogen and water. Yield of amino acid precursors by photons greatly depended on their wavelength. These results suggest that nitrogen-containing organic compounds like amino acid precursors were formed chiefly with high energy particles, not UV photons, in Titan or primitive Earth/Mars atmospheres where ammonia is not available as a predominant N-source.     

Digital system design in the presence of single event upsets

The authors consider the effects of single event upsets (SEUs) on digital systems, and show techniques for designing reliable systems with current levels of SEU protection. Three main systems are discussed: main memory, logic, and cache memory. A design for the main and cache memory subsystems that are SEU protected is also described. With SEU defined in bit days p, and using single error correction, it is shown that for all subsystems considered, an effective upset rate which is proportional to the product of p² and the time between corrections, or scrub time, can be obtained. Data for memory chip size and performance derived from the gallium-arsenide (GaAs) pilot lines funded by the Defense Advanced Research Projects Agency (DARPA) throughout the 1980s are used     

Design for a bioreactor with sunlight supply and operations systems for use in the space environment.

An experiment was carried out to determine the characteristics of an operations system that can support fast cultivation of algae at high densities in the weightlessness of space. The experiment was conducted in glass bioreactor tanks, in which light was supplied by radiator rods connected to optical fiber cables. The illumination areas of the tanks were 2600 cm2, 6000 cm2, and 9200 cm2 per liter of solution. The characteristics of O2-CO2 gas exchange, concentration and separation of chlorella in the growth medium, dialysis of ionic salts in the growth medium, etc. were examined. Chloralla ellipsoidea was used in the experiment, yielding the following results: (1) By increasing the ratio of illumination area to volume, growth rates of up to approximately 0.6 g/L h could be obtained in a highly concentrated solution (one that contains 20 g/L or more of algae). (2) The most suitable proportions of carbon dioxide and oxygen gases for growing algae quickly at high concentrations were found to be 10% CO2 and 10% O2 (by volume). (3) There was a high optimum concentration for fast cultivation, and the data obtained resembled the theoretical curve postulated by P. Behrens et al. (4) It was possible to exchange carbon dioxide and oxygen using gas-permeable membrane modules. (5) It was possible to separate the chlorella from the growth medium and recycle the medium.     

Sunlight supply and gas exchange systems in the microalgal bioreactor.

The bioreactor with sunlight supply system and gas exchange systems presented here has proved feasible in ground tests and shows much promise for space use as a CELSS device. Our chief conclusions concerning the specification of total system needed for a life support system for a man in a space station are the following. (1) Sunlight supply system: compactness and low electrical consumption. (2) Bioreactor system: high density and growth rate of chlorella. (3) Gas exchange system: enough for O2 production and CO2 assimilation.     

Turbulence in the region 80–120 km

Measurements of turbulent energy dissipation rates and eddy diffusion coefficients have been collated, and mean height profiles of fundamental turbulence parameters in the region 80–120 km are presented.     

Trapped antiprotons produced by cosmic rays in the Earth's magnetosphere.

The existence of significant fluxes of antiparticles in the Earth magnetosphere has been predicted on theoretical considerations in this article. These antiparticles (positrons or antiprotons) at several hundred kilometers of altitudes, we believe are not of direct extraterrestrial origin, but are the natural products of nuclear reactions of the high energy primary cosmic rays (CR) and trapped protons (TP) confined in the terrestrial radiation belt, with the constituents of terrestrial atmosphere. Extraterrestrial positrons and antiprotons born in nuclear reactions of the same CR particles passing through only 5-7 g/cm2 of interstellar matter, exhibit lower fluxes compared to the antiprotons born at hundreds of g/cm2 in the atmosphere, which when confined in the magnetic field of the Earth (in any other planet), get accumulated. We present the results of the computations of the antiproton fluxes at 10 MeV to several GeV energies due to CR particle interactions with the matter in the interstellar space, and also with the residual atmosphere at altitudes of approximately 1000 km over the Earth's surface. The estimates show that the magnetospheric antiproton fluxes are greater by two orders of magnitude compared to the extraterrestrial fluxes measured at energies <1-2 GeV.     

Genetic response of bacterial spores to very heavy ions.

Using spores of two Bacillus subtilis strains differing in repair capacity, we have studied repair and mutation induction in the spores after irradiation with very heavy ions up to uranium with specific particle energies up to 18.6 MeV/u. The results indicate that repair and mutation induction after heavy ion irradiation are closely related to each other and that both phenomena strongly depend on the atomic number and specific energy of the ions. The effects are discussed in comparison with results obtained after X-irradiation.