ERA-experiment "Space Biochemistry".

The general goal of the experiment was to study the response of anhydrobiotic (metabolically dormant) microorganisms (spores of Bacillus subtilis, cells of Deinococcus radiodurans, conidia of Aspergillus species) and cellular constituents (plasmid DNA, proteins, purple membranes, amino acids, urea) to the extremely dehydrating conditions of open space, in some cases in combination with irradiation by solar UV-light. Methods of investigation included viability tests, analysis of DNA damages (strand breaks, DNA-protein cross-links) and analysis of chemical effects by spectroscopic, electrophoretic and chromatographic methods. The decrease in viability of the microorganisms was as expected from simulation experiments in the laboratory. Accordingly, it could be correlated with the increase in DNA damages. The purple membranes, amino acids and urea were not measurably effected by the dehydrating condition of open space (in the dark). Plasmid DNA, however, suffered a significant amount of strand breaks under these conditions. The response of these biomolecules to high fluences of short wavelength solar UV-light is very complex. Only a brief survey can be given in this paper. The data on the relatively good survival of some of the microorganisms call for strict observance of COSPAR Planetary Protection Regulations during interplanetary space missions.     

Modeling of the very high velocity impact process with respect to in-situ ionization measurements

This overview deals with very high impact velocities, where complete vaporization of an impacting cosmic dust particle is to be expected upon expansion from the high pressure high temperature state behind the stopping shock (v > 15 km/s). The topics discussed are the mechanics and thermodynamics of compression, adiabatic release, equation of state and nonequilibrium states upon expansion. The case of very high particle porosity (ρ 1 g/cm³) and the case of very small dust masses (m < 10⁻¹⁷ g) are discussed from what one presently knows. The possibility of three body collisions in the expanding gas phase is discussed briefly. The effect of oblique impact is discussed with respect to its relevance to the ionization process. The numbers communicated are up to the highest “experimental” impact velocities (80 km/s, Halley mission). As one goes to lower impact velocities (20 < v < 30 km/s) there is still complete vaporization of the dust particle but ionization out of the bulk of the particle becomes low. Other than thermal processes may become important. Ideas are outlined to understand their physical nature.     

Molecular analysis of mutagenesis by high LET radiation.

Mutation induction by high linear energy transfer [LET] alpha particles and gamma-rays was scored in the human hamster hybrid [AL] cells. Southern blotting technique was used to analyse the molecular changes in the DNA from both the HGPRT- and S1- mutants. Dose dependent mutagenesis in the AL cells irradiated with the charged particles was higher by almost 20 fold at the S1 than the corresponding HGPRT locus. Southern analysis of the mutants induced by the high LET particles showed mostly multilocus deletion at both the HGPRT and S1 genes.     

Changes in gravitational force induce alterations in gene expression that can be monitored in the live, developing zebrafish heart.

Little is known about the effect of microgravity on gene expression, particularly in vivo during embryonic development. Using transgenic zebrafish that express the gfp gene under the influence of a beta-actin promoter, we examined the affect of simulated-microgravity on GFP expression in the heart. Zebrafish embryos, at the 18-20 somite-stage, were exposed to simulated-microgravity for 24 hours. The intensity of GFP fluorescence associated with the heart was then determined using fluorescence microscopy. Our measurements indicated that simulated-microgravity induced a 23.9% increase in GFP-associated fluorescence in the heart. In contrast, the caudal notochord showed a 17.5% increase and the embryo as a whole showed only an 8.5% increase in GFP-associated fluorescence. This suggests that there are specific effects on the heart causing the more dramatic increase. These studies indicate that microgravity can influence gene expression and demonstrate the usefulness of this in vivo model of 'reporter-gene' expression for studying the effects of microgravity.     

Top-level modeling of an ALS system utilizing object-oriented techniques.

The possible configuration of an Advanced Life Support (ALS) System capable of supporting human life for long-term space missions continues to evolve as researchers investigate potential technologies and configurations. To facilitate the decision process the development of acceptable, flexible, and dynamic mathematical computer modeling tools capable of system level analysis is desirable. Object-oriented techniques have been adopted to develop a dynamic top-level model of an ALS system.This approach has several advantages; among these, object-oriented abstractions of systems are inherently modular in architecture. Thus, models can initially be somewhat simplistic, while allowing for adjustments and improvements. In addition, by coding the model in Java, the model can be implemented via the World Wide Web, greatly encouraging the utilization of the model. Systems analysis is further enabled with the utilization of a readily available backend database containing information supporting the model. The subsystem models of the ALS system model include Crew, Biomass Production, Waste Processing and Resource Recovery, Food Processing and Nutrition, and the Interconnecting Space. Each subsystem model and an overall model have been developed. Presented here is the procedure utilized to develop the modeling tool, the vision of the modeling tool, and the current focus for each of the subsystem models.     

Escaping near-relativistic electron beams from the solar corona

Processes in the solar corona are prodigious accelerators of energetic ions, and electrons. The angular distribution, composition, and spectra of energetic particles observed near Earth gives information on the acceleration mechanisms. A class of energetic particle observations particularly useful in understanding the solar acceleration is the near-relativistic impulsive beam-like electron events. During five years of operation the Advanced Composition Explorer (ACE) has measured well over 400 electron events. Approximately 25% of these electron events are impulsive beam-like events that are released onto interplanetary field lines predominantly from western solar longitudes. We extend our initial 3 year study during the rise to solar maximum (Haggerty and Roelof, 2002; Simnett et al., 2002) to a five year statistical analysis of these beam-like energetic electron events in relationship to optical flares, microwave emission, soft X-ray emission, metric and decametric type-III radio bursts, and coronal mass ejections.     

Optimum Steady State Position, Velocity, and Acceleration Estimation Using Noisy Sampled Position Data

A one-dimensional tracking filter based on the Kalman filtering techniques for tracking of a dynamic target such as an aircraft is discussed. The target is assumed to be moving with constant acceleration and is acted upon by a plant noise which perturbs its constant acceleration motion. The plant noise accounts for maneuvers and/or other random factors. Analytical results for estimating optimum steady state position, velocity, and acceleration of the target are obtained.     

Adaptive pulse compression via MMSE estimation

Radar pulse compression involves the extraction of an estimate of the range profile illuminated by a radar in the presence of noise. A problem inherent to pulse compression is the masking of small targets by large nearby targets due to the range sidelobes that result from standard matched filtering. This paper presents a new approach based upon a minimum mean-square error (MMSE) formulation in which the pulse compression filter for each individual range cell is adaptively estimated from the received signal in order to mitigate the masking interference resulting from matched filtering in the vicinity of large targets. The proposed method is compared with the standard matched filter and least-squares (LS) estimation and is shown to be superior over a variety of stressing scenarios.     

Near infrared studies of the massive X-ray binary 2S 0114+65

We present near-infrared spectroscopy of the massive X-ray binary 2S 0114+650. These observations covering the spectral range 1.08–2.35 μm span the region where Paschen and Brackett series recombination lines of hydrogen are expected to be seen, namely, Paβ , Brγ and Br 10–17 lines. The absence of any of these lines in emission supports earlier inferences that the optical component in 2S 0114+650 is unlikely to be a Be star but rather a B type supergiant. Near-IR photometry gives J = 8.78, H = 8.53 and K = 7.96; these values show marginal variations from earlier reported measurements.     

Solid-state integrating detectors as an indicator of biological doses from HZE particles.

For interpretation of results obtained in future biological experiments in the International Space Station (ISS), biologically equivalent doses have to be determined using small-scale detectors without disturbing the surrounding radiation field. The detectors should be lightweight, stable, safe, and simple in handling. Solid-state integrating detectors (SSID) can satisfy these requirements. This paper demonstrates that combination of SSID such as thermoluminescence dosimeters and radiophotoluminescence glasses can be practically used for the evaluation of biologically equivalent doses. Statistical errors (type-A uncertainty) of this method will be satisfactorily small relative to those generally observed in biological responses. Permissible levels of systematic errors (type-B uncertainty) depend on dosimetry purposes (most-probable or conventional) and variability of biological responses.