Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems.

Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. Grant numbers: 99-04-96017, 00-07-9011.     

Transformation of a delta: Converting estuaries to lakes

In this century large-scale transformations have been carried out in the delta of the Rhine, Meuse and Scheldt in The Netherlands. The following principal consequences are considered: the loss of estuarine ecosystems, the change in the process of sedimentation and erosion, and the development of new aquatic and terrestrial ecosystems. The ecological consequences of the transformation of the Grevelingen estuary into a salt-water lake and some surprising developments are described briefly. The need for a limited restoration of the water exchange between the lake and the North Sea is indicated, as well as the major impacts of the exchange. The necessity for the maintenance or restoration of estuaries, the ecological development and the possibilities of the new lakes, and the management of these lakes by engineering structures are discussed. Careful land-use planning and ecosystem management, backed by research, are needed to make the fullest possible use of the potential offered by new lakes. It is also concluded that salt lakes have interesting perspectives.     

Attitude and Oribit Estimation Using Stars and Landmarks

An extended Kalman filter is used to process line-of-sight measurements to stars and known landmarks providing a statistical indication of performance in estimating spacecraft attitude, orbital ephemeris, and the bias drift of a set of three strapdown gyros. The landmark measurements were assumed to have been taken from the imagery of an Earth-observing multispectral scanner. It is shown that filtering of these noisy measurements results in highly accurate estimates of the above parameters. Results are given showing the sensitivity of performance to various system parameters such as star tracker accuracy, errors in the knowledge of landmark position, and number of stars and landmarks processed.     

The structural features of the germanium-silicon solid solution crystals obtained under microgravity

Structural features of germanium-silicon solid solution crystals have been investigated and silicon distribution in the crystals has been studied. All the crystals obtained under microgravity had, in spite of good external shape and facetting, a poorer internal structure than those obtained on Earth. The distribution of silicon have been shown to be non-uniform. High dislocation densities were observed.     

Microprocessor-Controlled Digital Shunt Regulator

A new approach to the design of power systems is presented in which a microprocessor is used as a controller for a digital shunt regulator (DSR). This approach meets the demands of future space and ground missions, i. e., high efficiency, high reliability, low weight, low volume, increased flexibility, and less development time. This approach responds to future demands by permitting realtime modification of system parameters for system optimization. This feature is especially important in the event of an anomaly. As the microprocessor need not be dedicated to the DSR, it can simultaneously be used for battery management and for charge regulator/discharge regulator control. This approach also reduces the component count, simplifies assembly and testing of the unit, results in significant time saving, and increases the reliability.     

Probability of Anomaly Expressions for Random Waveform Registration

Registration by integral-square error correlation of one-dimensional discrete-time waveforms which are treated as random processes with specified autocorrelation functions is considered. An important design parameter for this class of problems is the probability of anomaly (a false dip in the correlation function) because it gives an indication of system immunity to gross registration errors. Explicit expressions for this parameter are not possible, so bounds and appoximations must be derived. Two upper bounds and an approximation for the probability of anomaly are derived here. The use of these expressions is illustrated by an example. The relative utility of these performance indicators is shown for the example by comparison with actual values of the probability of anomaly obtained by computer simulation.     

Recent developments in ultraviolet filters and coatings

A number of new high efficiency optical coatings have recently been developed at wavelengths in the ultraviolet and vacuum ultraviolet for use in space science experiments. The development of these coatings has resulted in the design and manufacture of wavelength selective filters used in reflectance at near normal incidence that have high VUV reflectance with little near-UV and visible reflectance. Very narrow band transmission filters are now available at wavelengths as short as 1500Å with bandwidths as narrow as 35–40Å. In addition, high efficiency anti-reflection coatings as well as neutral density filters are now available at many wavelengths in this region.Material limitations, more severe than those in the visible, place certain restrictions on the size, wavelength, and degree of selectivity that is achievable with present technology. A summary of the current commercial technology along with the material limitations is presented.     

Enhanced resolution in simple radars

Many simple radars use fact-risetime pulses, but wide bandwidth does not translate into corresponding high resolution since the spectrum is far from flat. A scheme for enhancing down- and cross-range resolution of multiple targets through a two-step partial equalization of the spectrum is illustrated by detailed computer simulation, with emphasis on the tradeoffs between resolution and signal-to-noise ratios. Three examples are treated: two equal scattering centers, two unequal scattering centers, and compound target     

Sensor compensation for vehicle magnetic signatures

Most land vehicles whose positions need to be tracked are made primary of steel. The problem considered is that of finding locations within or on land vehicles where magnetic sensors can be mounted to measure heading accurately and of finding compensation methods that minimize the effect of the vehicle magnetic signature. Results obtained with various types of vehicles in several world locations are given. Advantages and disadvantages of pendulous mounting are considered, and vehicle magnetic changes caused by driving in areas of extremely strong magnetic fields such as those generated by DC-powered trains are discussed