Comparative metagenomics of two microbial mats at cuatro ciénegas basin I: ancient lessons on how to cope with an environment under severe nutrient stress

Abstract The Cuatro Ciénegas Basin (CCB) is an oasis in the desert of Mexico characterized by low phosphorus availability and by its great diversity of microbial mats. We compared the metagenomes of two aquatic microbial mats from the CCB with different nutrient limitations. We observed that the red mat was P-limited and dominated by Pseudomonas, while the green mat was N-limited and had higher species richness, with Proteobacteria and Cyanobacteria as the most abundant phyla. From their gene content, we deduced that both mats were very metabolically diverse despite their use of different strategies to cope with their respective environments. The red mat was found to be mostly heterotrophic, while the green mat was more autotrophic. The red mat had a higher number of transporters in general, including transporters of cellobiose and osmoprotectants. We suggest that generalists with plastic genomes dominate the red mat, while specialists with minimal genomes dominate the green mat. Nutrient limitation was a common scenario on the early planet; despite this, biogeochemical cycles were performed, and as a result the planet changed. The metagenomes of microbial mats from the CCB show the different strategies a community can use to cope with oligotrophy and persist. Key Words: Microbial mats-Metagenomics-Metabolism. Astrobiology 12, 648-658.     

The gamma-irradiation of aqueous hydrogen cyanide in the presence of ferrocyanide or ferricyanide: implications to prebiotic chemistry.

The gamma-irradiation of 0.1 M, O2-free, aqueous HCN was studied in the presence of ferrocyanide or ferricyanide in the concentration range 10(-3) - 10(-5) M. This study was carried out in order to get an insight into the possible role that cyanocomplexes of iron may have played in promoting prebiotic syntheses via the free-radical oligomerization of HCN. It was found that ferrocyanide or ferricyanide have no effect on the irradiation of 0.1 M HCN solutions at concentrations < or = 10(-4) M. At high concentrations, 10(-3) M, they lead to a marked decrease in the conversion of HCN. There was no significant difference due to the oxidation state of iron used, particularly at high doses > or = 100 kGy.     

Creating a space exploration industry

The article provides an overview of funding for space exploration to the Moon and Mars under NASA's direction, and presents an argument for the creation of a commercial space industry.     

Role of trace metal ions in chemical evolution. The case of free-radical reactions.

We have studied the effect of iron in the free-radical oligomerization of hydrogen cyanide and acetic acid, and found that iron(II) and iron(III) readily reduces or oxidizes free radicals, respectively. The transient species produced by these reactions do not induce a chain oligomerization process and, therefore, they protect the solute molecules from degradation. Analysis of the available kinetic data for the reactions of a variety of transition metal ions with free radicals indicate that transition metal ions behave similarly to iron. Since Fe, Zn and Mo are essential to all living organisms, and there seems to be no apparent difference in chemical reactivity among transition metal ions towards free radicals, we suggest that these metal ions probably protected the biomolecules from degradation induced by free-radical reactions in the later stages of chemical evolution.     

Oriented platform for the VEGA-probe to the Halley comet,position detection,working program

Two deep-space probes of the Venera type for the encounter with Halley's comet are being prepared in the Intercosmos program. 150 kg of scientific equipment will be onboard each sonde, including two TV cameras and two spectrometers. These instruments need precise orientation during the fly-by of the comet. For this purpose a stabilised platform is being developed in Czechoslovakia which will be installed onboard both probes and will be able to point to the target with precision 5 minutes of arc and maximum angular velocity 1°/second. A system of position detectors and the working program during the start and the fly-by are described.     

Estimation of cloud top height and effective cloud cover from infrared satellite soundings

The paper deals with a new method for simultaneous determination of cloud top height and effective cloud cover, using infrared radiance data of satellite-borne instruments. These cloud properties derived from the Selective Chopper Radiometer on the Nimbus 5 satellite are compared with nearly simultaneous observations by radiosondes and with satellite images. Encouraging results for Central-Europe during January, April, July, August and October 1974, as well as numerical simulations indicate that the method proposed here, would be useful also for global application. Another advantage of the described procedure are the small amount of computing time, and that no other data are required than 3 of infrared channel values, for each sounded spot.     

A quantitative assay of biologically important compounds in simulated primitive Earth experiments.

A CH4-N2-H2OV gas mixture was subjected to a high voltage (20 kV), high frequency (0.3 MHz) electric discharge. The energy input in the electric discharge was varied from 0.016 to 3.048 MJ mol-1. The chemical yields (G), expressed as the number of molecules formed or destroyed per 100 eV of energy input were calculated for several products. The G values calculated at the lowest energy input were (-CH4) = 6.48; (-N2) = 2.51; (C2H2) = 1.16; (HCN) = 0.215; (CH3CHO) = 0.115; (CH3CH2CHO) = 0.00161; (CH3(CH2)2CHO) = 0.0165; ((CH2CO2H)2) = 0.0000339; (CH4 --> Solid material) = 0.196; (N2 --> Solid material) = 0.00355. This is the first report in prebiotic studies in which the G values of various products in electric discharge experiments are determined. This type of study is needed in order to get a better insight into the relative role of electric discharges on the primitive Earth.     

Computer program prior used for orbit determination at the Ondřejov observatory

The computer program used mainly for determination of orbits of the Interkosmos satellites is described. Examples of results are given for Geos B and Interkosmos 17 satellites.     

Dynamic and figure parameters of Venus and Mars

Parameters of the best-fitting tri-axial ellipsoids representing external equipotential surfaces of Venus and Mars have been determined from satellite data. The dynamic consequence of the equatorial flattening of Venus has been discussed from the point of view of the s.c. synodic resonance rotation. The major gravitational anomalies of Venus have been interpreted, space locations and magnitudes of anomalous masses determined and their contribution to the second zonal Stokes' constant in the gravitational potential computed. The conclusions were done: The figure of the aphroditoid is strange even if there is a relatively small polar flattening; an equatorial “disc” of Venus is enormous. Recent space data do not support hypothesis that the Earth controls the spin of Venus.     

An estimation of the cooling process by infrared radiation in the atmosphere

Two procedure are presented for quantitative estimation of cloud cover (N), type of clouds (C), as well as base of clouds (C_b) and top of clouds (C^t) by using radiosonde data as well as satellite cloud pictures and radiation data. The data obtained in this way can be used as input data in the model for the estimation of the vertical profile of longwave radiative cooling.