Preservation of martian organic and environmental records: final report of the Mars biosignature working group

The Mars Science Laboratory (MSL) has an instrument package capable of making measurements of past and present environmental conditions. The data generated may tell us if Mars is, or ever was, able to support life. However, the knowledge of Mars' past history and the geological processes most likely to preserve a record of that history remain sparse and, in some instances, ambiguous. Physical, chemical, and geological processes relevant to biosignature preservation on Earth, especially under conditions early in its history when microbial life predominated, are also imperfectly known. Here, we present the report of a working group chartered by the Co-Chairs of NASA's MSL Project Science Group, John P. Grotzinger and Michael A. Meyer, to review and evaluate potential for biosignature formation and preservation on Mars. Orbital images confirm that layered rocks achieved kilometer-scale thicknesses in some regions of ancient Mars. Clearly, interplays of sedimentation and erosional processes govern present-day exposures, and our understanding of these processes is incomplete. MSL can document and evaluate patterns of stratigraphic development as well as the sources of layered materials and their subsequent diagenesis. It can also document other potential biosignature repositories such as hydrothermal environments. These capabilities offer an unprecedented opportunity to decipher key aspects of the environmental evolution of Mars' early surface and aspects of the diagenetic processes that have operated since that time. Considering the MSL instrument payload package, we identified the following classes of biosignatures as within the MSL detection window: organism morphologies (cells, body fossils, casts), biofabrics (including microbial mats), diagnostic organic molecules, isotopic signatures, evidence of biomineralization and bioalteration, spatial patterns in chemistry, and biogenic gases. Of these, biogenic organic molecules and biogenic atmospheric gases are considered the most definitive and most readily detectable by MSL.     

On the stabilization of ribose by silicate minerals

The RNA-world theory hypothesizes that early Earth life was based on the RNA molecule. However, the notion that ribose, the sugar in RNA, is unstable still casts a serious doubt over this theory. Recently, it has been found that the silicate-mediated formose reaction facilitates the stabilization of ribose. Using accurate quantum chemical calculations, we determined the relative stability of the silicate complexes of arabinose, lyxose, ribose, and xylose with the intent to determine which would form predominantly from a formose-like reaction. Five stereoisomers were investigated for each complex. The stereoisomers of 2:1 ribose-silicate are the more stable ones, to the extent that the least stable of these is even more stable than the most stable stereoisomer of the other 2:1 sugar-silicate complexes. Thus, thermodynamically, a formose-like reaction in the presence of silicate minerals should preferentially form the silicate complex of ribose over the silicate complex of arabinose, lyxose, and xylose.     

Design of a canard configured TransCruiser using CEASIOM

CEASIOM is a multidisciplinary software environment for aircraft design that has been developed as part of the European Framework 6 SimSAC project. It closely integrates discipline-specific tools such as those used for CAD, grid generation, CFD, stability analysis and control system design. The environment allows the user to take an initial design from geometry definition and aerodynamics generation through to full six degrees of freedom simulation and analysis. Key capabilities include variable fidelity aerodynamics tools and aeroelasticity modules. The purpose of this paper is to demonstrate the potential of CEASIOM by presenting the results of a Design, Simulate and Evaluate (DSE) exercise applied to a novel, project specific, transonic cruiser configuration called the TCR. The baseline TCR configuration is first defined using conventional methods, which is then refined and improved within the CEASIOM software environment. A wind tunnel model of this final configuration was then constructed, tested and used to verify the results generated using CEASIOM.     

Comparison of satellite orbit ephemerides for use in GPS meteorology

This paper discusses GPS (Global Position System) meteorology. The research presented is based on a comparison of values of precipitable water vapour PWV, based on GPS measurements using final and predicted ephemerides of satellite orbits. We analysed recent year’s improvement in predicting ephemerides. We compared the data outputs from a radiosonde using GPS receiver measurements directly from the meteorological station from which the radiosondes were launched. The results indicate a high quality of the predicted ephemerides. This finding makes predicted ephemerides highly usable for near real-time estimations of PWV. To use PWV in meteorological forecast applications, this high speed of PWV values supply is necessary.     

Lower ionosphere response to external forcing: A brief review

There are two ways of external forcing of the lower ionosphere, the region below an altitude of about 100 km: (1) From above, which is directly or indirectly of solar origin. (2) From below, which is directly or indirectly of atmospheric origin. The external forcing of solar origin consists of two general factors – solar ionizing radiation variability and space weather. The solar ionization variability consist mainly from the 11-year solar cycle, the 27-day solar rotation and solar flares, strong flares being very important phenomenon in the daytime lower ionosphere due to the enormous increase of the solar X-ray flux resulting in temporal terminating of MF and partly LF and HF radio wave propagation due to heavy absorption of radio waves. Monitoring of the sudden ionospheric disturbances (SIDs – effects of solar flares in the lower ionosphere) served in the past as an important tool of monitoring the solar activity and its impacts on the ionosphere. Space weather effects on the lower ionosphere consist of many different but often inter-related phenomena, which govern the lower ionosphere variability at high latitudes, particularly at night. The most important space weather phenomenon for the lower ionosphere is strong geomagnetic storms, which affect substantially both the high- and mid-latitude lower ionosphere. As for forcing from below, it is caused mainly by waves in the neutral atmosphere, i.e. planetary, tidal, gravity and infrasonic waves. The most important and most studied waves are planetary and gravity waves. Another channel of the troposphere coupling to the lower ionosphere is through lightning-related processes leading to sprites, blue jets etc. and their ionospheric counterparts. These phenomena occur on very short time scales. The external forcing of the lower ionosphere has observationally been studied using predominantly ground-based methods exploiting in various ways the radio wave propagation, and by sporadic rocket soundings. All the above phenomena are briefly mentioned and some of them are treated in more detail.     

Field emission characteristics of gold dust grains

The space plasma environment usually contains charged dust grains. The grain charge is an important parameter determining its migration through the space, coagulation, formation of dust clouds and so on. The knowledge of its charge is thus one of the basic information we want to know. There are several emission processes leading to both positive and negative charges, among others photoemission, all kinds of secondary emissions, field emissions, etc. The present study is focused on a laboratory simulation of emissions caused by impacts by energetic ions. Our experiment is based on the Paul trap which gives us an opportunity to catch a single dust grain for several days inside the vacuum vessel and exposed it by electron/ion beams. This experimental approach allows us to separate an individual charging process. We have chosen spherically shaped gold grains and discussed the processes leading to the limitation of the grain charge. We suggested that the implantation of charging ions leads to deformations of the grain surface. The deformations enhance the local electric field strength that becomes sufficient for the field ionization.     

Relation between galactic and solar cosmic radiation at aviation altitude

Cosmic radiation bombards us at high altitude with ionizing particles; the radiation has a galactic component, which is normally dominant, and a component of solar origin. Cosmic ray particles are the primary source of ionization in the atmosphere above 1 km; below 1 km radon is a dominant source of ionization in many areas.     

Some ecological mechanisms to generate habitability in planetary subsurface areas by chemolithotrophic communities: the Río Tinto subsurface ecosystem as a model system

Chemolithotrophic communities that colonize subsurface habitats have great relevance for the astrobiological exploration of our Solar System. We hypothesize that the chemical and thermal stabilization of an environment through microbial activity could make a given planetary region habitable. The MARTE project ground-truth drilling campaigns that sampled cryptic subsurface microbial communities in the basement of the Río Tinto headwaters have shown that acidic surficial habitats are the result of the microbial oxidation of pyritic ores. The oxidation process is exothermic and releases heat under both aerobic and anaerobic conditions. These microbial communities can maintain the subsurface habitat temperature through storage heat if the subsurface temperature does not exceed their maximum growth temperature. In the acidic solutions of the Río Tinto, ferric iron acts as an effective buffer for controlling water pH. Under anaerobic conditions, ferric iron is the oxidant used by microbes to decompose pyrite through the production of sulfate, ferrous iron, and protons. The integration between the physical and chemical processes mediated by microorganisms with those driven by the local geology and hydrology have led us to hypothesize that thermal and chemical regulation mechanisms exist in this environment and that these homeostatic mechanisms could play an essential role in creating habitable areas for other types of microorganisms. Therefore, searching for the physicochemical expression of extinct and extant homeostatic mechanisms through physical and chemical anomalies in the Mars crust (i.e., local thermal gradient or high concentration of unusual products such as ferric sulfates precipitated out from acidic solutions produced by hypothetical microbial communities) could be a first step in the search for biological traces of a putative extant or extinct Mars biosphere.     

Temporal changes in fluid chemistry and energy profiles in the vulcano island hydrothermal system

In June 2003, the geochemical composition of geothermal fluids was determined at 9 sites in the Vulcano hydrothermal system, including sediment seeps, geothermal wells, and submarine vents. Compositional data were combined with standard state reaction properties to determine the overall Gibbs free energy (DeltaG(r) ) for 120 potential lithotrophic and heterotrophic reactions. Lithotrophic reactions in the H-O-N-S-C-Fe system were considered, and exergonic reactions yielded up to 120 kJ per mole of electrons transferred. The potential for heterotrophy was characterized by energy yields from the complete oxidation of 6 carboxylic acids- formic, acetic, propanoic, lactic, pyruvic, and succinic-with the following redox pairs: O(2)/H(2)O, SO(4) (2)/H(2)S, NO(3) ()/NH(4) (+), S(0)/H(2)S, and Fe(3)O(4)/Fe(2+). Heterotrophic reactions yielded 6-111 kJ/mol e(). Energy yields from both lithotrophic and heterotrophic reactions were highly dependent on the terminal electron acceptor (TEA); reactions with O(2) yielded the most energy, followed by those with NO(3) (), Fe(III), SO(4) (2), and S(0). When only reactions with complete TEA reduction were included, the exergonic lithotrophic reactions followed a similar electron tower. Spatial variability in DeltaG(r) was significant for iron redox reactions, owing largely to the wide range in Fe(2+) and H(+) concentrations. Energy yields were compared to those obtained for samples collected in June 2001. The temporal variations in geochemical composition and energy yields observed in the Vulcano hydrothermal system between 2001 and 2003 were moderate. The largest differences in DeltaG(r) over the 2 years were from iron redox reactions, due to temporal changes in the Fe(2+) and H(+) concentrations. The observed variations in fluid composition across the Vulcano hydrothermal system have the potential to influence not only microbial diversity but also the metabolic strategies of the resident microbial communities.     

Searching for life on Mars: selection of molecular targets for ESA's aurora ExoMars mission

The European Space Agency's ExoMars mission will seek evidence of organic compounds of biological and non-biological origin at the martian surface. One of the instruments in the Pasteur payload may be a Life Marker Chip that utilizes an immunoassay approach to detect specific organic molecules or classes of molecules. Therefore, it is necessary to define and prioritize specific molecular targets for antibody development. Target compounds have been selected to represent meteoritic input, fossil organic matter, extant (living, recently dead) organic matter, and contamination. Once organic molecules are detected on Mars, further information is likely to derive from the detailed distribution of compounds rather than from single molecular identification. This will include concentration gradients beneath the surface and gradients from generic to specific compounds. The choice of biomarkers is informed by terrestrial biology but is wide ranging, and nonterrestrial biology may be evident from unexpected molecular distributions. One of the most important requirements is to sample where irradiation and oxidation are minimized, either by drilling or by using naturally excavated exposures. Analyzing regolith samples will allow for the search of both extant and fossil biomarkers, but sequential extraction would be required to optimize the analysis of each of these in turn.