Physiological, biochemical and molecular processes associated with gravitropism in roots of maize.

This research aims to characterize regulation of the principal cytosolic protein kinases in maize, cultivar 'Merit' root tips, since much evidence indicates that stimuli which modulate the gravitropic response in this system act through regulation of activity of these enzymes. To this end, we have cloned a maize protein kinase belonging to a group of plant protein kinases with a catalytic domain similar in primary structure to the second messenger-regulated protein kinases known in animal and fungal systems. However, both the unique structural features conserved among plant protein kinases in this group, and lack of evidence for cyclic nucleotide signalling in plants point to operation of a novel protein kinase regulatory mechanism in plants. In order to test effects of possible regulators on protein kinase activity, we developed a sensitive method for detecting regulation of autophosphoryl labelling of protein kinases in unfractionated maize protein extracts. Regulation of protein kinase autophosphorylation in these extracts was different from that known in animals and fungi, further suggesting operation of unique protein kinase regulatory mechanisms in plants. Previous research has shown that light, or factors modulated by light, regulate plant protein kinase activity. We found that protein kinase activity was co-immunoprecipitated with the plant photoreceptor phytochrome, and was associated with phytochrome by high-affinity chemical interactions. Far-red reversibility of red-light regulation of phytochrome phosphorylation by the associated protein kinase indicates that it may modulate or transduce the light signals which lead to gravitropic sensitivity in 'Merit' maize.     

Performance and reliability of the NASA Biomass Production Chamber.

The Biomass Production Chamber (BPC) at the Kennedy Space Center is part of the Controlled Ecological Life Support System (CELSS) Breadboard Project. Plants are grown in a closed environment in an effort to quantify their contributions to the requirements for life support. Performance of this system is described. Also, in building this system, data from component and subsystem failures are being recorded. These data are used to identify problem areas in the design and implementation. The techniques used to measure the reliability will be useful in the design and construction of future CELSS. Possible methods for determining the reliability of a green plant, the primary component of a CELSS, are discussed.     

Galactic cosmic ray transport methods: past, present, and future.

The development of the theory of high charge and energy (HZE) ion transport is reviewed. The basic solution behavior and approximation techniques will be described. An overview of the HZE transport codes currently available at the Langley Research Center will be given. The near term goal of the Langley program is to produce a complete set of one-dimensional transport codes. The ultimate goal is to produce a set of complete three-dimensional codes which have been validated in the laboratory and can be applied in the engineering design environment. Recent progress toward completing these goals is discussed.     

Cognitive deficits induced by 56Fe radiation exposure.

Exposing rats to particles of high energy and charge (e.g., 56Fe) disrupts neuronal systems and the behaviors mediated by them; these adverse behavioral and neuronal effects are similar to those seen in aged animals. Because cognition declines with age, and our previous study showed that radiation disrupted Morris water maze spatial learning and memory performance, the present study used an 8-arm radial maze (RAM) to further test the cognitive behavioral consequences of radiation exposure. Control rats or rats exposed to whole-body irradiation with 1.0 Gy of 1 GeV/n high-energy 56Fe particles (delivered at the alternating gradient synchrotron at Brookhaven National Laboratory) were tested nine months following exposure. Radiation adversely affected RAM performance, and the changes seen parallel those of aging. Irradiated animals entered baited arms during the first 4 choices significantly less than did controls, produced their first error sooner, and also tended to make more errors as measured by re-entries into non-baited arms. These results show that irradiation with high-energy particles produces age-like decrements in cognitive behavior that may impair the ability of astronauts to perform critical tasks during long-term space travel beyond the magnetosphere.     

Battery charger design for the Columbus MTFF power system

A novel pulsewidth-modulated (PWM) dc-dc converter topology is proposed for the battery charge regulator (BCR) of the Columbus Man-Tended Free-Flyer (MTFF) power system. The system is a regulated bus system. Bus voltage control is implemented at the input of the BCR. The use of a conventional buck topology with PWM conductance control at the input results in a second-order behavior. A study of new PWM dc-dc converter topologies has been made to attain a suitable topology. The proposed converter topology is designed and a breadboard including the control loop has been built and tested. The experimental results show that the converter operates according to the design constraints.     

Oscillations of axial plant organs.

The tips of roots and shoots commonly show lateral movements as they grow forwards. These occur as both circumnutations (with long periods and large amplitudes) and micronutations (with short periods and small amplitudes). Their properties are reviewed, with emphasis on roots, and possible ways in which they could be regulated are discussed. The mechanisms could include long-range controls (for circumnutations) that depend on transmissible signals using steps common to gravitropism, and short-range controls (for micronutations) that operate within the elongation zone. The former are a property of the apex as a whole, while the latter may be confined to localized groups of cells. Simulation of nutations is presented with a view to isolating key physiological processes. However, this approach is limited by the current inadequate understanding of the growth mechanisms involved.     

A program to measure new energetic particle nuclear interaction cross sections.

The Transport Collaboration, consisting of researchers from institutions in France, Germany, Italy and the USA, has established a program to make new measurements of nuclear interaction cross sections for heavy projectiles (Z > or = 2) in targets of liquid H2, He and heavier materials. Such cross sections directly affect calculations of galactic and solar cosmic ray transport through matter and are needed for accurate radiation hazard assessment. To date, the collaboration has obtained data using the LBL Bevalac HISS facility with 20 projectiles from 4He to 58Ni in the energy range 393-910 MeV/nucleon. Preliminary results from the analysis of these data are presented here and compared to other measurements and to cross section prediction formulae.     

Suboptimal joint probabilistic data association

A significant problem in multiple target tracking is the hit-to-track data association. A hit is a received signal from a target or background clutter which provides positional information. If an incorrect hit is associated with a track, that track could diverge and terminate. Prior methods for this data association problem include various optimal and suboptimal two-dimensional assignment algorithms which make hit-to-track associations. Another method is to assign a weight for the reasonable hits and use a weighted centroid of those hits to update the track. The method of weighting the hits is known as joint probabilistic data association (JPDA). The authors review the JPDA approach and a simple ad hoc approximation and then introduce a new suboptimal JPDA algorithm. Examples which compare an optimal two-dimensional assignment algorithm with the ad hoc and the new suboptimal JPDA formulation are given     

Performance analysis for DOA estimation algorithms: unification,simplification, and observations

Subspace based direction-of-arrival (DOA) estimation has motivated many performance studies, but limitations such as the assumption of an infinite amount of data and analysis of individual algorithms generally exist in these performance studies. The authors have previously proposed a unified performance analysis based on a finite amount of data and achieved a tractable expression for the mean-squared DOA estimation error for the multiple signal classification (MUSIC). Min-Norm, estimation of signal parameters using rotational invariance techniques (ESPRIT), and state-space realization algorithms. However, this expression uses the singular values and vectors of a data matrix, which are obtained by the highly nonlinear transformation of the singular value decomposition (SVD). Thus the effects of the original data parameters such as numbers of sensors and snapshots, source coherence and separations were not explicitly analyzed. The authors unify and simplify this previous result and derive a unified expression based on the original data parameters. They analytically observe the effects of these parameters on the estimation error     

Target motion analysis with multiple arrays: performance analysis

The performance analysis of source trajectory estimation using measurements provided by multiple platforms (or arrays) is studied. In numerous practical situations, the maneuvering ability of the receiver (e.g., a ship towing linear arrays) is limited, leading to the assumption that the observer motion is rectilinear and uniform. Even if this hypothesis appears quite restrictive, practical and tactical considerations fully justify its interest. This leads to consider multiple (platform) target motion analysis (denoted MTMA) and to analyze the performance of such trajectory estimation methods