Induction and repair of DNA strand breaks in bovine lens epithelial cells after high LET irradiation.

The lens epithelium is the initiation site for the development of radiation induced cataracts. Radiation in the cortex and nucleus interacts with proteins, while in the epithelium, experimental results reveal mutagenic and cytotoxic effects. It is suggested that incorrectly repaired DNA damage may be lethal in terms of cellular reproduction and also may initiate the development of mutations or transformations in surviving cells. The occurrence of such genetically modified cells may lead to lens opacification. For a quantitative risk estimation for astronauts and space travelers it is necessary to know the relative biological effectiveness (RBE), because the spacial and temporal distribution of initial physical damage induced by cosmic radiation differ significantly from that of X-rays. RBEs for the induction of DNA strand breaks and the efficiency of repair of these breaks were measured in cultured diploid bovine lens epithelial cells exposed to different LET irradiation to either 300 kV X-rays or to heavy ions at the UNILAC accelerator at GSI. Accelerated ions from Z=8 (O) to Z=92 (U) were used. Strand breaks were measured by hydroxyapatite chromatography of alkaline unwound DNA (overall strand breaks). Results showed that DNA damage occurs as a function of dose, of kinetic energy and of LET. For particles having the same LET the severity of the DNA damage increases with dose. For a given particle dose, as the LET rises, the numbers of DNA strand breaks increase to a maximum and then reach a plateau or decrease. Repair kinetics depend on the fluence (irradiation dose). At any LET value, repair is much slower after heavy ion exposure than after X-irradiation. For ions with an LET of less than 10,000 keV micrometers-1 more than 90 percent of the strand breaks induced are repaired within 24 hours. At higher particle fluences, especially for low energetic particles with a very high local density of energy deposition within the particle track, a higher proportion of non-rejoined breaks is found, even after prolonged periods of incubation. At the highest LET value (16,300 keV micrometers-1) no significant repair is observed. These LET-dependencies are consistent with the current mechanistic model for radiation induced cataractogenesis which postulates that genomic damage to the surviving fraction of epithelial cells is responsible for lens opacification.     

Energetic ion emission for active spacecraft control

Future space missions aiming at the accurate measurement of cold plasmas and DC to very low frequency electric fields will require that the potential of their conductive surfaces be actively controlled to be near the ambient plasma potential. In the near-Earth space these spacecraft are usually solar-cell powered; consequently, parts of their surface are most of the time exposed to solar photons. Outside the plasmasphere, a positive surface potential due the dominance of surface-emitted photoelectrons over ambient plasma electrons is to be expected. Photo- and ambient electrons largely determine the potential and positive values between a few Volts up to 100 V have been observed. Active ion emission is the obvious solution of this problem. A liquid metal ion emitter and a saddle field ion emitter are nearing the stage of flight unit fabrication. We will attempt to clamp the spacecraft potential to values close to the plasma potential. We present first results from vacuum chamber tests and describe the emission behaviour and characteristics of emitters producing, respectively, In⁺ and N₂⁺ beams with an energy of ≥ 5 keV.     

The detection of energetic cometary and solar particles by the EPONA instrument on the Giotto mission

EPONA is an energetic particle detector system incorporating totally depleted silicon surface barrier layer detectors. Active and passive background shielding will be employed and, by applying various techniques, particles of different species, including electrons, protons, alpha particles and pick-up ions of cometary origin may be detected over a wide spectrum of energies extending from the tens of KeV into the MeV range.

The instrument can operate in two modes namely (a) in a cruise phase or storage mode and (b) in a real time mode. During the real time mode, observations at high spatial (octosectoring) and temporal (0.5s) resolution in the cometary environment permit studies to be made of accelerated particles at the bow shock and/or in the tail of the comet. In conjunction with magnetic field measurements on board Giotto, observations of energetic electrons and their anisotropies can determine whether the magnetic field lines in the cometary tail are open or closed. Further, the absorption of low energy solar particles in the cometary atmosphere can be measured and such data would provide an integral value of the pertaining gas and dust distribution. Solar particle background measurements during encounter may also be used to correct the measurements of other spacecraft borne instruments potentially vulnerable to such radiation.

Solar particle flux measurements, obtained during the cruise phase will, when combined with simultaneous observations made by other spacecraft at different heliographic longitudes, provide information concerning solar particle propagation in the corona and in interplanetary space.     

Long-term survival of bacterial spores in space.

On board of the NASA Long Duration Exposure Facility (LDEF), spores of Bacillus subtilis in monolayers (10(6)/sample) or multilayers (10(8)/sample) were exposed to the space environment for nearly six years and their survival was analyzed after retrieval. The response to space parameters, such as vacuum (10(-6) Pa), solar electromagnetic radiation up to the highly energetic vacuum-ultraviolet range (10(9) J/m2) and/or cosmic radiation (4.8 Gy), was studied and compared to the results of a simultaneously running ground control experiment. If shielded against solar ultraviolet (UV)-radiation, up to 80 % of spores in multilayers survive in space. Solar UV-radiation, being the most deleterious parameter of space, reduces survival by 4 orders of magnitude or more. However, up to 10(4) viable spores were still recovered, even in completely unprotected samples. Substances, such as glucose or buffer salts serve as chemical protectants. With this 6 year study in space, experimental data are provided to the discussion on the likelihood of "Panspermia".     

The characteristics of sharp (small-scale) boundaries of solar wind plasma and magnetic field structures

We investigate properties of large (>20%) and sharp (<10 min) solar wind ion flux changes using INTERBALL-1 and WIND plasma and magnetic field measurements from 1996 to 1999. These ion flux changes are the boundaries of small-scale and middle-scale solar wind structures. We describe the behavior of the solar wind velocity, temperature and interplanetary magnetic field (IMF) during these sudden flux changes. Many of the largest ion flux changes occur during periods when the solar wind velocity is nearly constant, so these are mainly plasma density changes. The IMF magnitude and direction changes at these events can be either large or small. For about 55% of the ion flux changes, the sum of the thermal and magnetic pressure are in balance across the boundary. In many of the other cases, the thermal pressure change is significantly more than the magnetic pressure change. We also attempted to classify the types of discontinuities observed.     

Annual solar UV exposure and biological effective dose rates on the Martian surface.

The ultraviolet (UV) environment of Mars has been investigated to gain an understanding of the variation of exposure throughout a Martian year, and link this flux to biological effects and possible survival of organisms at the Martian surface. To gain an idea of how the solar UV radiation varies between different regions, including planned landing sites of two future Mars surface missions, we modelled the total solar UV surface flux throughout one Martian year for two different dust scenarios. To understand the degree of solar UV stress on micro-organisms and/or molecules essential for life on the surface of Mars, we also calculated the biologically effective dose (BED) for T7 and Uracil in relevant wavelength regions at the Martian surface as a function of season and latitude, and discuss the biological survival rates in the presence of Martian solar UV radiation. High T7/Uracil BED ratios indicate that even at high latitudes where the UV flux is significantly reduced, the radiation environment is still hostile for life due to the persisting UV-C component of the flux.     

Problems with the concept of the inflationary universe

The picture of an exponentially increasing, “inflationary” phase of the early universe (Guth 1981; Linde 1982; Albrecht and Steinhardt 1982) may point the way to an understanding of our present universe without reference to extremely specific initial conditions. The model rests, however, on several assumptions which deserve critical examination.     

Dayside reconnection during IMF northward: A possible foreshock effect

The northward and southward orientation of the interplanetary magnetic field (IMF) is usually considered as providing the external boundary conditions in the solar wind interaction with the Earth's magnetopause but it is the magnetic field in the magnetosheath that interacts with the Earth's magnetic field. In this paper, we consider the possibility that the wave activity in the foreshock region may affect the magnetic field orientation in the magnetosheath with time scales that might be geomagnetically effective. If magnetosheath magnetic field becomes disturbed on plasma streamlines which are connected to the quasi-parallel bow shock and foreshock, the magnetic field orientation on the inner magnetosheath may differ significantly from the undisturbed IMF. We present a model of dayside reconnection which may occur when the IMF northward and illustrate its effects on the erosion of the magnetopause.     

Pigment composition and concentrations within the plant (Ceratophyllum demersum L.) component of the STS-89 C.E.B.A.S. Mini-Module spaceflight experiment.

The Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) Mini-Module, a Space Shuttle middeck locker payload which supports a variety of aquatic inhabitants (fish, snails, plants and bacteria) in an enclosed 8.6 L chamber, was tested for its biological stability in microgravity. The aquatic plant, Ceratophyllum demersum L., was critical for the vitality and functioning of this artificial mini-ecosystem. Its photosynthetic pigment concentrations were of interest due to their light harvesting and protective functions. "Post-flight" chlorophyll and carotenoid concentrations within Ceratophyllum apical segments were directly related to the quantities of light received in the experiments, with microgravity exposure (STS-89) failing to account for any significant deviation from ground control studies.     

Solar UV irradiance variation during cycles 22 and 23

The Solar Ultraviolet Spectral Irradiance Monitor (SUSIM) aboard the Upper Atmosphere Research Satellite (UARS) has been measuring solar UV irradiances since October 1991, a period which includes the decline of solar cycle 22 followed by the rise of cycle 23. Daily solar measurements include scans over the wavelength range 115–410 nm at 1.1 nm resolution. As expected, the measured time series of UV irradiances exhibit strong periodicities in solar cycle and solar rotation. For all wavelengths, the UV irradiance time series are similar to that of the Mg II core-to-wing ratio. During solar cycle 22, the irradiance of the strong Ly- line varied by more than a factor of two. The peak-to-peak irradiance variation declined with increasing wavelength, reaching 10% just below the Al edge at 208 nm. Between the Al edge and 250 nm the variation was 6–7%. Above 250 nm, the variation declines further until none is observed above 290 nm. Preliminary results for the first portion of cycle 23 indicate that the far UV below the Al edge is rising at about the same rate as the Mg II index while the irradiances in the Ly- emission line and for wavelengths longer than the Al edge are rising more slowly — even after accounting for the lower level of activity of cycle 23.