Effects of carbon ions on primary cultures of mouse brain cells.

Primary mixed cultures of astrocytes and microglia were obtained from neonatal mice, and were irradiated with high-LET carbon ions. Immunohistochemical staining showed astrocytes survived more prominently than microglia. Tagged with specific antibodies, astrocytes and microglia surviving after irradiation were counted by flow cytometry. Decreases in the number of microglia and astrocytes were detected at a dose as small as 2 Gy when Day 5 cultures were irradiated with 13 keV/micrometer carbon ions. When the cultures were irradiated on Day 10, the dose-dependent decrease of microglia was more prominent for 13 keV/micrometer carbon ions than 70 keV/micrometer carbon ions. Astrocytes showed a marginal decrease at Day 10 and Day 14. We concluded that microglia are more sensitive than astrocytes to carbon ions and X-rays, and that the radiosensitivity of microglia depends on both differentiation/proliferation status and radiation quality.     

External Cue Effects on Memory for Spatial Location within a Rotated Task Field

Abstract

We propose a mathematical model of the Path Integration (PI) process. Its core assumption is that orientations of a path are summarized by circular probability distributions. We compare our model with classical, deterministic models of PI and find that, although they are indistinguishable in terms of information encoded, the probabilistic model is more parsimonious when considering navigation strategies. We show how sensory events can enrich the probability distributions memorized, resulting in a continuum of navigation strategies, from PI to stimulus-triggered response. We analyze the combination of circular probability distributions (e.g., multicue fusion), and demonstrate that, contrary to the linear case, adding orientation cues does not always increase reliability of estimates. We discuss experimental predictions entailed by our model.     

Short-Lived Radionuclides in Meteorites: Constraints on Nebular Timescales for the Production of Solids

Variations in the abundances of short-lived radionuclides such as ²⁶Al (τ_1/2 ≈ 0.74 Ma) and ⁵³Mn (τ_1/2 ≈ 3.7 Ma) in meteoritic solids may be used to infer relative formation intervals of these solids in the nebula at precisions of less than 1 Ma. In a strict chronometric interpretation of the isotopic variations, whereby criteria such as spatial and temporal isotopic homogeneity and closed system isotopic evolution are met, solid formation occurred in the nebula for at least several million years. This is longer than some theoretical and astronomical estimates for the duration of the active nebula. The evidence for live ⁴¹Ca (τ_1/2 ≈ 0.10 Ma) in meteoritic inclusions further indicates that the onset of solid formation occurred quite early, perhaps within a few hundred thousand years after the onset of the collapse of the sun's parent molecular cloud. Failure of the chronometric interpretation may arise for a variety of reasons, including but not limited to, the late, inhomogeneous injection of material from a nearby stellar source or the local production of short-lived radionuclides by an energetic particle irradiation, e. g., from T Tauri (X-wind) or galactic cosmic ray sources. Although some isotopic evidence exists that the criteria required for a strict chronometric interpretation are not met by each of the short-lived chronometers, there is no compelling reason to shorten the interval of solid formation in the nebula to less than 1 Ma. This revised version was published online in June 2006 with corrections to the Cover Date.     

Atomic Physics for Atmospheric Composition Measurements

The atomic physics relevant to the interpretation of solar spectra produced by plasmas at temperatures ≳ 105 K are discussed. Methods for determining relative abundance ratios are presented and examples provided from the Coronal Diagnostic Spectrometer on board SOHO. In particular, the Fe/Si ratio in the corona is found to be close to photospheric; the Mg/Ne ratio in the transition region is found to vary by an order of magnitude in different solar features. The Mg/Ne ratios in supergranule cell centres and the network are separated for the first time, although no significant differences are found. This revised version was published online in June 2006 with corrections to the Cover Date.     

Benefits and risks of using electrodynamic tethers to de-orbit spacecraft

By using electrodynamic drag to greatly increase the orbital decay rate, an electrodynamic space tether can remove spent or dysfunctional spacecraft from low Earth orbit (LEO) rapidly and safely. Moreover, the low mass requirements of such tether devices make them highly advantageous compared to conventional rocket-based de-orbit systems. However, a tether system is much more vulnerable to space debris impacts than a typical spacecraft and its design must be proved to be safe up to a certain confidence level before being adopted for potential applications. To assess space debris related concerns, in March 2001 a new task (Action Item 19.1) on the “Potential Benefits and Risks of Using Electrodynamic Tethers for End-of-life De-orbit of LEO Spacecraft” was defined by the Inter-Agency Space Debris Coordination Committee (IADC). Two tests were proposed to compute the fatal impact rate of meteoroids and orbital debris on space tethers in circular orbits, at different altitudes and inclinations, as a function of the tether diameter to assess the survival probability of an electrodynamic tether system during typical de-orbiting missions. IADC members from three agencies, the Italian Space Agency (ASI), the Japan Aerospace Exploration Agency (JAXA) and the US National Aeronautics and Space Administration (NASA), participated in the study and different computational approaches were specifically developed within the framework of the IADC task. This paper summarizes the content of the IADC AI 19.1 Final Report. In particular, it introduces the potential benefits and risks of using tethers in space, it describes the assumptions made in the study plan, it compares and discusses the results obtained by ASI, JAXA and NASA for the two tests proposed. Some general conclusions and recommendations are finally extrapolated from this massive and intensive piece of research.     

Gravisensing in plants and fungi.

The principle of establishing and maintaining a gravitropic set point angle depends on gravisensing and a subsequent cascade of events that result in differential elongation of the responsive structures. Since gravity acts upon masses, the gravisensing mechanisms of all biological systems must follow the same principle, namely the sensing of some force due to differential acceleration of the perceiving entity and a reference structure. This presentation will demonstrate that gravisensing can be accomplished by various means, ranging from cytoskeletal organization, mechano-elastic stress to perturbation of electric signals. However, several arguments indicate that sedimentation of either dense plastids (statoliths), the entire protoplast, or a combination of these represents the primary step in graviperception in plants. In fungi, nuclei and cytoskeletal proteins are believed to form a network capable of gravisensing but sedimenting organelles that may function as statoliths have been identified. Theoretical and practical limitations of gravisensing and detection of acceleration forces necessitate microgravity experiments to identify the primary perceptor, subsequent biochemical mechano-transduction, and biological response processes.     

Influence of long-term hyper-gravity on the reactivity of succinic acid dehydrogenase and NADPH-diaphorase in the central nervous system of fish: a histochemical study.

In the course of a densitometric evaluation, the histochemically demonstrated reactivity of succinic acid dehydrogenase (SDH) and of NADPH-diaphorase (NADPHD) was determined in different brain nuclei of two teleost fish (cichlid fish Oreochromis mossambicus, swordtail fish Xiphophorus helleri), which had been kept under 3g hyper-gravity for 8 days. SDH was chosen since it is a rate limiting enzyme of the Krebs cycle and therefore it is regarded as a marker for metabolic and neuronal activity. NADPHD reactivity reflects the activity of nitric oxide synthase. Nitric oxide (NO) is a gaseous intercellular messenger that has been suggested to play a major role in several different in vivo models of neuronal plasticity including learning. Within particular vestibulum-connected brain centers, significant effects of hyper-gravity were obtained, e.g., in the magnocellular nucleus, a primary vestibular relay ganglion of the brain stem octavolateralis area, in the superior rectus subdivision of the oculomotoric nucleus and within cerebellar eurydendroid cells, which in teleosts possibly resemble the deep cerebellar nucleus of higher vertebrates. Non-vestibulum related nuclei did not respond to hyper-gravity in a significant way. The effect of hyper-gravity found was much less distinct in adult animals as compared to the circumstances seen in larval fish (Anken et al., Adv. Space Res. 17, 1996), possibly due to a development correlated loss of neuronal plasticity.     

HIGH SPEED FLOW DESIGN USING THE THEORY OF OSCULATING CONES AND AXISYMMETRIC FLOWS

Ｃｏｎｆｉｇｕｒａｔｉｏｎａｅｒｏｄｙｎａｍｉｃｄｅｓｉｇｎｃｏｎｃｅｐｔｓａｒｅａｉｍｅｄａｔｔｈｅｄｅｆｉｎｉｔｉｏｎｏｆｆｉｎｄｉｎｇａｉｒｆｒａｍｅａｎｄｐｒｏｐｕｌｓｉｏｎｃｏｍｐｏｎｅｎｔｇｅｏｍｅｔｒｉｅｓｗｉｔｈａｃｏｎｔｒｏｌｅｄｆ...     

On the nature of coronal loops above the quiet sun network

The structure and dynamics of a box in a stellar corona can be modeled employing a 3D MHD model for different levels of magnetic activity. Depending on the magnetic flux through the surface the nature of the resulting coronal structures can be quite different. We investigate a model of an active region for two sunspots surrounded by magnetic field patches comparable in magnetic flux to the sunspots. The model results in emission from the model corona being concentrated in loop structures. In Gudiksen and Nordlund (2005) the loops seen in EUV and X-ray emission outline the magnetic field, following the general paradigm. However, in our model, where the magnetic field is far from a force-free state, the loops seen in X-ray emission do not follow the magnetic field lines. This result is of interest especially for loops as found in areas where the magnetic field emerging from active regions interacts with the surrounding network.