Microorganisms and biomolecules in space environment experiment ES 029 on Spacelab-1.

Bacterial spores are proper test organisms for studying problems of space biology and exobiology. During the Spacelab 1 mission, studies on the limiting factors for survival of Bacillus subtilis spores in free space have been performed. An exposure tray on the pallet of Spacelab 1 accomodated 316 samples of dry spores for treatment with space vacuum and/or the following selected wavelengths of solar UV: > 170 nm, 220 nm, 240nm, 260nm and 280 nm. After recovery, inactivation, mutation induction, reparability, and photochemical damages in DNA and protein have been studied. The results contribute to the understanding of the mechanisms of increased UV sensitivity of bacterial spores in vacuo and to a better assessment of the chance of survival of resistant forms in space and of interplanetary transfer of life.     

On the conditions of gas inclusions formation in melts under zero gravity state (after “Pirin” experiment)

Thermodynamics relations have been considered under which gas inclusions may form in melts (closed systems), due to both volatility of components and the presence of dissolved gases. It has been shown that in the melts with sufficient volatility of components, gas inclusions may form under equilibrium conditions if the ampoule is wetted well by the melt, whereas in case of low wettability they form under non-equilibrium state only. The foaming in the tellurium-selenium system has been analysed (“Pirin”, KP-4 experiment).In the melts containing dissolved gases, gas inclusions form under conditions of deviation from equilibrium and it is the main mechanism of outgassing. It has been demonstrated that the melt foaming method using hydrid compounds may be applied to the materials exhibiting a hysteresis of temperature dependence of diluted hydrogen concentration. Production of foam aluminium under zero gravity state has been considered as an example (“Pirin”, SP-3 experiment).     

Organisation of a unified system of energetic calibration of X-ray experiments

X-ray data obtained by the Prognoz 5,6,7 and 8 hard X-ray photometers are compared with the measurements carried out by similar instruments aboard the Solrad 11, ISEE 3, SMM and Hinotori satellites. Using the method of relative amplitude analysis, the apparent disagreement in the energy discrimination level calibration between the instruments is pointed out. The results of the comparison and the possible sources of disagreement are given. We suggest an international effort be made to develop a system of uniform pre-launch calibration of photometers based on a reference calibration source.     

The organic aerosols of Titan

A dark reddish organic solid, called tholin, is synthesized from simulated Titanian atmospheres by irradiation with high energy electrons in a plasma discharge. The visible reflection spectrum of this tholin is found to be similar to that of high altitude aerosols responsible for the albedo and reddish color of Titan. The real (n) and imaginary (k) parts of the complex refractive index of thin films of Titan tholin prepared by continuous D.C. discharge through a 0.9 N₂/0.1 CH₄ gas mixture at 0.2 mb is determined from x-ray to microwave frequencies. Values of n (1.65) and k (0.004 to 0.08) in the visible are consistent with deductions made by ground-based and spaceborne observations of Titan. Many infrared absorption features are present in k(λ), including the 4.6 μm nitrile band. Molecular analysis of the volatile component of this tholin was performed by sequential and non-sequential pyrolytic gas chromatography/mass spectrometry. More than one hundred organic compounds are released; tentative identifications include saturated and unsaturated aliphatic hydrocarbons, substituted polycyclic aromatics, nitriles, amines, pyrroles, pyrazines, pyridines, pyrimidines, and the purine, adenine. In addition, acid hydrolysis produces a racemic mixture of biological and non-biological amino acids. Many of these molecules are implicated in the origin of life on Earth, suggesting Titan as a contemporary laboratory environment for prebiological organic chemistry on a planetary scale.     

Flare build up: 21 May 1980

The decaying solar active region that crossed the central meridian on May 20, 1980 at latitude S13° produced a major flare (2B/X1) at 2054 on May 21. This region was a target of the international Flare Buildup Study and was well observed. The buildup was characterized by little flare activity during two days prior to the major flare but a great deal of activity in the filament that separated the opposite magnetic polarities of the active region. Large proper motions of sunspots and magnetic fields suggest that the magnetic field was stressed prior to the flare. The immediate trigger of the flare appears to have been an eruption of new magnetic flux in the center of the active region. The new flux erupted in a configuration that decreased the net flux of the active region and contributed to the decay of the region.     

Particle and Joule heating of the neutral polar thermosphere in cusp region using Atmosphere Explorer-C satellite measurements

Simultaneous measurements taken by instruments on the Atmosphere Explorer - C satellite were used to compare electron and proton particle energy deposition, Joule heating, and neutral density perturbations in the region of the cusp.Altitude profiles of Joule heating, electron energy deposition, and electron density are derived using measurements taken by the satellite as input to a computer model. Electric fields are calculated using ion drift measurements. Figures are presented for a representative orbital pass.A peak Joule heating rate of 0.059 Wm^?2 occurred in the cusp region with a peak of 0.025 Wm^?2 in the evening auroral electrojet. Peak volume heating rates corresponding to these regions were 1.4 × 10^?6Wm^?3 and 7.10^?7 Wm^?3, both occurring at an altitude of 115 km. Particle energy deposition was about an order of magnitude less than Joule heating. Large neutral density perturbations are related to regions of heating.     

Localization performance of arrays subject to phase errors

The passive localisation of radiating sources using an array subject to random perturbations in sensor phases is presented. All source signals as well as additive noises observed at the sensors are assumed to be independent identically distributed zero-mean Gaussian random processes. Cramer-Rao bounds are derived for source bearings and ranges for the phase errors at each sensor. It is shown that accurate phase calibration can be achieved when the number of sources exceeds a certain minimum. The locations of the calibrating sources need not be known a priori and need only satisfy mild regularity conditions. A calibration procedure is proposed which uses maximum-likelihood techniques     

Computational fluid dynamics and material processing (buoyancy and thermocapillary convections in melts during directional crystallization)

Modelisation and solution of heat and mass transfer problems relevant for material processing are generally hard to be handled, as they often involve 3D unsteady flows, viscous mixtures, phase changes, moving liquid-solid fronts, deforming liquid-gas interfaces, etc.… For space applications, material processing benefits of reduced buoyancy convection but can be faced to a strongly increased complexity due to variable g, mainly in manned flight.

Computational techniques used to analyse fluid motions in material processing, accounting for free surface, crystallization front and bulk convection in melt, are reviewed with emphasis to directional crystallization. Hydrodynamics stability and bifurcation analysis are shown to be useful complementary tools for correlating data, and for a better understanding of the physical laws. This last point will be illustrated in the case of the onset of oscillations in metallic melts.     

Identification of specific gravity sensitive signal transduction pathways in human A431 carcinoma cells.

Epidermal growth factor (EGF) activates a well characterized signal transduction cascade in human A431 epidermoid carcinoma cells. The influence of gravity on EGF-induced EGF-receptor clustering and early gene expression as well as on actin polymerization and actin organization have been investigated. Different signalling pathways induced by the agents TPA, forskolin and A23187 that activate gene expression were tested for sensitivity to gravity. EGF-induced c-fos and c-jun expression were decreased in microgravity. However, constitutive beta-2 microglobulin expression remained unaltered. Under simulated weightlessness conditions EGF- and TPA-induced c-fos expression was decreased, while forskolin- and A23187-induced c-fos expression was independent of the gravity conditions. These results suggest that gravity affects specific signalling pathways. Preliminary results indicate the EGF-induced EGF-receptor clustering remained unaltered irrespective of the gravity conditions. Furthermore, the relative filamentous actin content of steady state A431 cells was enhanced under microgravity conditions and actin filament organization was altered. Under simulated weightlessness actin filament organization in steady state cells as well as in EGF-treated cells was altered as compared to the 1 G reference experiment. Interestingly the microtubule and keratin organization in untreated cells showed no difference with the normal gravity samples. This indicates that gravity may affect specific components of the signal transduction circuitry.