An examination of the carbon isotope effects associated with amino acid biosynthesis

Stable carbon isotope ratios (delta(13)C) were determined for alanine, proline, phenylalanine, valine, leucine, isoleucine, aspartate (aspartic acid and asparagine), glutamate (glutamic acid and glutamine), lysine, serine, glycine, and threonine from metabolically diverse microorganisms. The microorganisms examined included fermenting bacteria, organotrophic, chemolithotrophic, phototrophic, methylotrophic, methanogenic, acetogenic, acetotrophic, and naturally occurring cryptoendolithic communities from the Dry Valleys of Antarctica. Here we demonstrated that reactions involved in amino acid biosynthesis can be used to distinguish amino acids formed by life from those formed by nonbiological processes. The unique patterns of delta(13)C imprinted by life on amino acids produced a biological bias. We also showed that, by applying discriminant function analysis to the delta(13)C value of a pool of amino acids formed by biological activity, it was possible to identify key aspects of intermediary carbon metabolism in the microbial world. In fact, microorganisms examined in this study could be placed within one of three metabolic groups: (1) heterotrophs that grow by oxidizing compounds containing three or more carbon-to-carbon bonds (fermenters and organotrophs), (2) autotrophs that grow by taking up carbon dioxide (chemolitotrophs and phototrophs), and (3) acetoclastic microbes that grow by assimilation of formaldehyde or acetate (methylotrophs, methanogens, acetogens, and acetotrophs). Furthermore, we demonstrated that cryptoendolithic communities from Antarctica grouped most closely with the autotrophs, which indicates that the dominant metabolic pathways in these communities are likely those utilized for CO(2 )fixation. We propose that this technique can be used to determine the dominant metabolic types in a community and reveal the overall flow of carbon in a complex ecosystem.     

Infrared spectra and radiation stability of H2O2 ices relevant to Europa

In this paper we present spectra of H2O2-containing ices in the near- and mid-infrared (IR) regions. Spectral changes on warming are shown, as is a comparison of near-IR bands of H2O and H2O2-containing ices. An estimate of the A-value (absolute intensity) for the largest near- IR feature of H2O2 is given. Radiation-decay half-lives are reported for 19 K and 80 K, and are related to the surface radiation doses on Europa. The radiation data show that H2O2 destruction is slower at 80 K than 19 K, and are consistent with the claim that icy material in the outermost micrometer of Europa's surface has been heavily processed by radiation.     

Microorganisms metabolizing on clay grains in 3-km-deep Greenland basal ice

We have discovered > 10(8) microbial cells/cm3 attached to clay grains in the bottom 13 m of the GISP2 (Greenland Ice Sheet Project) ice core. Their concentration correlates with huge excesses of CO2 and CH4. We show that Fe-reducing bacteria produce most of the excess CO2 and methanogenic archaea produce the excess CH4. The number of attached cells per clay grain is proportional to grain perimeter rather than to area, which implies that nutrients are accessed at grain edges. We conclude that Fe-reducing microbes immobilized on clay surfaces metabolize via "shuttle" molecules that transport electrons to grain edges, where they reduce Fe(III) ions at edges to Fe(II) while organic acid ions are oxidized to CO2. Driven by the concentration gradient, electrons on Fe(II) ions at grain edges "hop" to Fe(III) ions inward in the same edges and oxidize them. The original Fe(III) ions can then attach new electrons from shuttle molecules at the edges. Our mechanism explains how Fe-reducers can reduce essentially all Fe(III) in clay minerals. We estimate that the Fe(III) in clay grains in the GISP2 silty ice can sustain Fe-reducing bacteria at the ambient temperature of -9 degrees C for approximately 10(6) years. F420 autofluorescence imaging shows that > 2.4% of the cells are methanogens, which account for the excess methane.     

巴尔迪克-径向铆接--满足最精确的铆接成形要求

巴尔迪克的铆接法可铆接以前被认为不可铆接的材料,并能得到完美的铆接面。材料沿径向滚动可防止产生扭应力,从而得到高质量的铆接面并具有很高的抗疲劳强度     

Modeling Particle Fluxes and Absorbed Dose for Protection from Cosmic Rays Using the SHIELD Transport Code

Cosmic Research - A special version of the SHIELD transport code has been developed intended for radiation protection purposes in space. The calculation of the fluxes of primary and secondary...     

Investigation of Satellite Trace (ST) and Multi-reflected Echo (MRE) ionogram signatures and its possible correlation to nighttime spread F development from Cyprus over the solar mini-max (2009–2016)

Multi-reflected echoes (MREs) and satellite traces (STs) are referred in literature as ionogram signatures of Travelling Ionospheric Disturbances (TIDs) which is a phenomenon that apparently drives spread F development mainly at nighttime mid-latitude ionosphere. A long-term statistical study has been undertaken to investigate the morphological aspect of these signatures over the lower midlatitude European station of Nicosia, Cyprus (35.19°N, 33.38°E geographic; magnetic dip. 29.38°N) by inspecting all ionograms recorded by the DPS-4D digisonde in the interval 2009–2016. The results underline the systematic manifestation of these TID signatures over Cyprus with a possible (although not quite clear) solar activity dependence and a distinctive seasonal and diurnal occurrence rate with a seasonal peak of STs during summer and of MREs during January to April. Based on the experimental results of the present study, the seasonal occurrence rate of MREs and STs is found to increase by 75% and 56% during high solar activity periods. Satellite traces are well known ionogram signatures of TIDs and mostly correlated to the nighttime spread F formation. The occurrence of mid-latitude spread Fs over European longitude sector normally increases during summer. The occurrences of TIDs are also prominent at this interval of the year over nighttime mid-latitude ionosphere. The presence of MREs as an ionogram signature of TIDs over mid-latitude ionosphere is unique in nature.     

Statistical evaluation of GLONASS amplitude scintillation over low latitudes in the Brazilian territory

The ionospheric scintillation, generated by the ionospheric plasma irregularities, affects the radio signals that pass through it. Their effects are widely studied in the literature with two different approaches. The first one deals with the use of radio signals to study and understand the morphology of this phenomenon, while the second one seeks to understand and model how much this phenomenon interferes in the radio signals and consequently in the services to which these systems work. The interest of several areas, particularly to those that are life critical, has increased using the concept of satellite multi-constellation, which consists of receiving, processing and using data from different navigation and positioning systems. Although there is a vast literature analyzing the effects of ionospheric scintillation on satellite navigation systems, the number of studies using signals received from the Russian satellite positioning system (named GLONASS) is still very rare. This work presents for the first time in the Brazilian low-latitude sector a statistical analysis of ionospheric scintillation data for all levels of magnetic activities obtained by a set of scintillation monitors that receive signals from the GLONASS system. In this study, data collected from four stations were used in the analysis; Fortaleza, Presidente Prudente, São José dos Campos and Porto Alegre. The GLONASS L-band signals were analyzed for the period from December 21, 2012 to June 20, 2016, which includes the peak of the solar cycle 24 that occurred in 2014. The main characteristics of scintillation presented in this study include: (1) the statistical evaluation of seasonal and solar activity, showing the chances that an user on similar geophysical conditions may be susceptible to the effects of ionospheric scintillation; (2) a temporal analysis based on the local time distribution of scintillation at different seasons and intensity levels; and (3) the evaluation of number of simultaneously affected channels and its effects on the dilution of precision (DOP) for GNSS users are also presented in order to alert the timetables in which navigation will be most susceptible to such effects, as well as statistics on simultaneously affected channels. Relevant results about these statistical characteristics of scintillation are presented and analyzed providing relevant information about availability of a navigation system.     

Strength design model for thin-walled structures

In this paper, the finite element analysis of the stress-strain state for cylindrical structures is discussed. In this case, various fundamental hypotheses can be used in the framework of a model that is of interest for design and optimization. Numerical examples are presented to evaluate the possibilities of this model.