Repair of DNA double-strand breaks and cell killing by charged particles.

It has been suggested that it is not simple double-strand breaks (dsb) but the non-reparable breaks which correlate well with the high biological effectiveness of high LET radiations for cell killing (Kelland et al., 1988; Radford, 1986). We have compared the effects of charged particles on cell death in 3 pairs of cell lines which are normal or defective in the repair of DNA dsbs. For the cell lines SL3-147, M10, and SX10 which are deficient in DNA dsb repair, RBE values were close to unity for cell killing induced by charged particles with linear energy transfer (LET) up to 200 keV/micrometer and were even smaller than unity for the LET region greater than 300 keV/micrometer. The inactivation cross section (ICS) increased with LET for all 3 pairs. The ICS of dsb repair deficient mutants was always larger than that of their parents for all the LET ranges, but with increasing LET the difference in ICS between the mutant and its parent became smaller. Since a small difference in ICS remained at LET of about 300 keV/micrometer, dsb repair may still take place at this high LET, even if its role is apparently small. These results suggest that the DNA repair system does not play a major role in protection against the attack of high LET radiations and that a main muse of cell death is non-reparable dsb which are produced at a higher yield compared with low LET radiations. No correlation was observed between DNA content or nuclear area and ICS.     

Adaptive detection schemes in compound-Gaussian clutter

Radar detection of coherent pulse trains embedded in compound-Gaussian disturbance with partially known statistics is discussed. We first give a thorough derivation of two recently proposed adaptive detection structures. Next, we derive a different detection scheme exploiting the assumption that the clutter is wide-sense stationary. Resorting to the theory of circulant matrices, in fact, we demonstrate that the estimation of the structure of the clutter covariance matrix can be reduced to the estimation of its eigenvalues, which in turn can be (efficiently) done via fast Fourier transform codes. After a thorough performance assessment, mostly carried on via computer simulations, the results show that the newly proposed detector achieves better performance than the two previously introduced adaptive detectors. Moreover, a sensitivity analysis shows that, even though this detector does not strictly guarantee the constant false alarm rate property with respect to the clutter covariance matrix, it is robust, in the sense that its performance is only slightly affected by variations in the clutter temporal correlation     

Effect of track structure and radioprotectors on the induction of oncogenic transformation in murine fibroblasts by heavy ions

The oncogenic potential of high-energy ⁵⁶Fe particles (1 GeV/nucleon) accelerated with the Alternating Gradient Synchrotron at the Brookhaven National Laboratory was examined utilizing the mouse C3H 10T1/2 cell model. The dose-averaged LET for high-energy ⁵⁶Fe is estimated to be 143 keV/μm with the exposure conditions used in this study. For ⁵⁶Fe ions, the maximum relative biological effectiveness (RBE_max) values for cell survival and oncogenic transformation were 7.71 and 16.5 respectively. Compared to 150 keV/μm ⁴He nuclei, high-energy ⁵⁶Fe nuclei were significantly less effective in cell killing and oncogenic induction. The prostaglandin E₁ analog misoprostol, an effective oncoprotector of C3H 10T1/2 cells exposed to X rays, was evaluated for its potential as a radioprotector of oncogenic transformation with high-energy ⁵⁶Fe. Exposure of cells to misoprostol did not alter ⁵⁶Fe cytotoxicity or the rate of ⁵⁶Fe-induced oncogenic transformation.     

EXOSAT LE-CMA observations of the Vela supernova remnant

Presented here are Exosat LE1-CMA images of fields in the Vela SNR. Soft X-ray emission is observed in the north of the remnant indicating a filamentry structure. No X-ray emission was seen in two fields to the west and south-west which have optical filaments but were not studied by Einstein. The Vela pulsar is observed and is significantly broader than a point source indicating the presence of a small nebula as seen by the Einstein HRI. The flux seen from the point source in the CMA is consistent with blackbody radiation from a 10⁶ degree neutron star of approx. 10 km radius. A bright ridge of emission is seen north of the pulsar which may be part of the extended synchroton nebula seen in the 2–10 keV range.     

Ground assisted rendezvous with geosynchronous satellites for the disposal of space debris by means of Earth-oriented tethers

Previous studies have shown that extended length Earth-oriented tethers in the geosynchronous (GEO) region can be used to re-orbit satellites to disposal orbits. One such approach involves the extension of a GEO based tether, collection of a debris object, and retraction of the tether, which transfers the retracted configuration to a higher energy orbit for debris disposal. The re-extension of the tether after debris disposal returns the configuration to the near-GEO altitude. The practical feasibility of such a system depends on the ability to collect GEO debris objects, attach them to a deployed tether system, and retract the tethers for transfer to the disposal orbits.This study addresses the collection and delivery of debris objects to the deployed tether system in GEO. The investigation considers the number, type and the characteristics of the debris objects as well as the collection tug that can be ground controlled to detect, rendezvous and dock with the debris objects for their delivery to the tethers system.A total of more than 400 objects are in drift orbits crossing all longitudes either below or above the geostationary radius. More than 130 objects are also known to librate around the stable points in GEO with periods of libration up to five or more years. A characterization of the position and velocity of the debris objects relative to the collection tug is investigated. Typical rendezvous performance requirements for uncooperative GEO satellites are examined, and the similarities with other approaches such as the ESA's CX-OLEV commercial mission proposal to extend the life of geostationary telecommunication satellites are noted.     

New evidence for strong non-thermal effects in Tycho’s supernova remnant

For the case of Tycho’s supernova remnant (SNR), we present the relation between the blast wave and contact discontinuity radii calculated within the nonlinear kinetic theory of cosmic ray (CR) acceleration in SNRs. It is demonstrated that these radii are confirmed by recently published Chandra measurements which show that the observed contact discontinuity radius is very close to the shock radius. Therefore a consistent explanation of these observations can be given in terms of efficient CR acceleration which makes the medium more compressible.     

Tissue responses to low protracted doses of high LET radiations or photons: early and late damage relevant to radio-protective countermeasures.

Early and late murine tissue responses to single or fractionated low doses of heavy charged particles, fission-spectrum neutrons or gamma rays are considered. Damage to the hematopoietic system is emphasized, but results on acute lethality, host response to challenge with transplanted leukemia cells and life-shortening are presented. Low dose rates per fraction were used in some neutron experiments. Split-dose lethality studies (LD 50/30) with fission neutrons indicated greater accumulation of injury during a 9 fraction course (over 17 days) than was the case for gamma-radiation. When total doses of 96 or 247 cGy of neutrons or gamma rays were given as a single dose or in 9 fractions, a significant sparing effect on femur CFU-S depression was observed for both radiation qualities during the first 11 days, but there was not an earlier return to normal with dose fractionation. During the 9 fraction sequence, a significant sparing effect of low dose rate on CFU-S depression was observed in both neutron and gamma-irradiated mice. CFU-S content at the end of the fractionation sequence did not correlate with measured LD 50/30. Sustained depression of femur and spleen CFU-S and a significant thrombocytopenia were observed when a total neutron dose of 240 cGy was given in 72 fractions over 24 weeks at low dose rates. The temporal aspects of CFU-S repopulation were different after a single versus fractionated neutron doses. The sustained reduction in the size of the CFU-S population was accompanied by an increase in the fraction in DNA synthesis. The proliferation characteristics and effects of age were different for radial CFU-S population closely associated with bone, compared with the axial population that can be readily aspirated from the femur. In aged irradiated animals, the CFU-S proliferation/redistribution response to typhoid vaccine showed both an age and radiation effect. After high single doses of neutrons or gamma rays, a significant age- and radiation-related deficiency in host defense mechanisms was detected by a shorter mean survival time following challenge with transplantable leukemia cells. Comparison of dose-response curves for life shortening after irradiation with fission-spectrum neutrons or high energy silicon particles indicated high initial slopes for both radiation qualities at low doses, but for higher doses of silicon, the effect per Gy decreased to a value similar to that for gamma rays. The two component life-shortening curve for silicon particles has implications for the potential efficacy of radioprotectants. Recent studies on protection against early and late effects by aminothiols, prostaglandins, and other compounds are discussed.     

A Simple Real-Time Incoherent Optical Correlator

The Theory and experimental results of a simple optical correlator are given. The optical components consist only of an incoherent source, condensing, collimating, and transforming lenses, and a photodetector. For real-time operation, a reference signal on a photographic transparency is correlated with an unknown signal on an ultrasonic light modulator. The output is a function of time proportional to the correlation function's amplitude rather than the squared amplitude generated by other optical correlators. When used for correlation in radar signal processing, the optical processor may be used for both pulse coding and decoding (pulse expansion and compression) and the decoded signal is shown to be described by Woodward's ambiguity function. The experimental results demonstrated the validity of the theory in both real and scaled time. However, developmental work is still required to improve the operation.     

Biological UV dosimeters in simulated space conditions.

Polycrystalline uracil thin layers participate in the phage and uracil response (PUR) experiment, assigned to the biological dosimetry of the extraterrestrial solar radiation on the International Space Station (ISS). In ground based experiments (experiment verification tests), the following space parameters were simulated and studied: temperature, vacuum and short wavelength UV (UV-C, down to 200 nm) radiation. The closed uracil samples proved to be vacuum-tight for 7 days. In the tested temperature range (from -20 to +40 degrees C) the uracil samples are stable. The kinetic of dimer formation (dimerization) and reversion (monomerization) of uracil dimers due to short wavelength UV radiation was detected, the monomerization efficiency of the polychromatic deuterium lamp is higher than that of the germicidal lamp. A mathematical model describing the kinetic of monomerization-dimerization was constructed. Under the influence of UV radiation the dimerization-monomerization reactions occur simultaneously, thus the additivity law of the effect of the various wavelengths is not applicable.