Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

Usefulness of daily +2Gz load as a countermeasure against physiological problems during weightlessness.

Adaptation to head-down-tilt bed rest as a simulated microgravity leads to an abnormality of reflex control of circulation, hypovolemia and reduction of exercise capacity. We hypothesized that this cardiovascular deconditioning and reduction of exercise capacity could be prevented by a daily 1 hr centrifugation at +2Gz. To test this hypothesis, twenty healthy male subjects underwent 4 day of 6 degrees head-down-tilt bed rest. Ten of them were exposed to a +2Gz load for up to 30 min twice per day (the Gz group). The remaining 10 were not exposed to a Gz load (the no-Gz group). We estimated autonomic cardiovascular control by power spectral analysis of blood pressure and R-R interval variability, and baroreflex regulation by the transfer function analysis and the sequence method, before and after bed rest. Further, we measured hematocrit as an index of changes in plasma volume and maximal oxygen consumption as an index of exercise capacity, before and after bed rest. Result: In the no-Gz group, heart rate increased after bed rest. The high frequency power of R-R interval variability as an index of cardiac parasympathetic nervous activity, baroreflex gains estimated by transfer function analysis and the sequence method as index of the integrated arterial-cardiac baroreflex function decreased significantly. Associated with these changes, the ratio of low to high frequency power of R-R as an indicator of cardiac sympathovagal balance tended to increase after bed rest in the no-Gz group. However, those showed no significant changes after bed rest in the Gz group. Hematocrit increased after bed rest in the no-Gz group. It also tended to increase in the Gz group, however it did not achieve statistical significance. Maximal oxygen consumption decreased significantly to similar extent in both the groups. Conclusion: This result suggested that 1) a daily 1hr +2Gz load produced by a centrifuge might eliminate the changes in autonomic cardiovascular control during simulated weightlessness; 2) furthermore, it might partly reverse hypovolemia induced by bed rest; 3) however, it could not prevent the decreases in exercise capacity.     

Local-area helioseismology as a diagnostic tool for solar variability

Dynamical and thermal variations of the internal structure of the Sun can affect the energy flow and result in variations in irradiance at the surface. Studying variations in the interior is crucial for understanding the mechanisms of the irradiance variations. “Global” helioseismology based on analysis of normal mode frequencies, has helped to reveal radial and latitudinal variations of the solar structure and dynamics associated with the solar cycle in the deep interior. A new technique, - “local-area” helioseismology or heliotomography, offers additional potentially important diagnostics by providing three-dimensional maps of the sound speed and flows in the upper convection zone. These diagnostics are based on inversion of travel times of acoustic waves which propagate between different points on the solar surface through the interior. The most significant variations in the thermodynamic structure found by this method are associated with sunspots and complexes of solar activity. The inversion results provide evidence for areas of higher sound speed beneath sunspot regions located at depths of 4–20 Mm, which may be due to accumulated heat or magnetic field concentrations. However, the physics of these structures is not yet understood. Heliotomography also provides information about large-scale stable longitudinal structures in the solar interior, which can be used in irradiance models. This new diagnostic tool for solar variability is currently under development. It will require both a substantial theoretical and modeling effort and high-resolution data to develop new capabilities for understanding mechanisms of solar variability.     

Multiple hypothesis track maintenance with possibly unresolved measurements

In surveillance problems dense clutter/dense target situations call for refined data association and tracking techniques. In addition, closely spaced targets may exist which are not resolved. This phenomenon has to be considered explicitly in the tracking algorithm. We concentrate on two targets which temporarily move in close formation and derive a generalization of MHT methods on the basis of a simple resolution model.     

The Detection Performance of the Siebert and Dicke-Fix CFAR Radar Detectors

The Siebert and the Dicke-fix CFAR radar detectors, used to maintain a constant false alarm rate (CFAR) in radar receivers under very similar circumstances, are considered. The Siebert detector represents the maximum-likelihood detection procedure for a signal in Gaussian noise of unknown power level, whereas the Dicke-fix makes use of a bandpass limiter to normalize the input and thus ensure a constant false alarm rate. The detection performance of the two detectors is determined and a comparison shows that over a wide range of parameters, the Dicke-fix introduces a loss which is approximately 1 B larger than for the Siebert detector.     

The observation of the galactic anticenter region by the balloon borne gamma-ray telescope Natalya-I

This paper presents the results of measurements of gamma radiation with energies above 5 MeV, from the galactic anticenter region. The balloon-borne gamma ray telescope “Natalya-I”, was launched on 6 November, 1980 from the Tata Institute of Fundamental Research's Balloon Facility (Hyderabad, India) and reached ceiling altitude of 35 km. The results on the accelerator calibration of the telescope, using a “tagged” gamma ray beam are also presented.     

INMARSAT integrity channels for global navigation satellite systems

The transmission of integrity information using a signal format compatible with the Global Positioning System (GPS) and relayed through a geostationary satellite repeater, which will be critical in achieving high integrity and availability of global navigation by satellite is discussed. The inclusion of navigation repeaters designed to fulfil this function, the next generation of INMARSAT spacecraft, INMARSAT-3 is examined. The global navigation satellite system (GNSS) integrity channel (GIC) will employ pseudorandom codes in the same family as, but distinct from, the codes reserved by GPS. The data format of the basic integrity channel is designed to convey user range error information for 24 to 40 satellites. A closed-loop timing compensation technique will be used at the uplinking Earth station, to make the signal's clock and carrier Doppler variations identical to those that would result from an onboard signal source. Therefore, the INMARSAT-3 satellites will increase the number of useful navigation satellites available to any user, and can also function as sources of precise timing. There is also a possibility that wide area differential corrections can be carried on the same signal     

On the role of meteoritic impacts in the formation of organic molecules

It is suggested that the UV radiation, and shock and plasma phenomena which accompanied the hypervelocity impacts of solid bodies (meteorites and comets) onto the surface of the young Earth may have contributed to the synthesis of prebiotic organic molecules in the primitive atmosphere in a larger amount than was thought previously. The mechanisms responsible for this synthesis are discussed using information obtained from recent experimental and theoretical work on macroscopic hypervelocity impacts.     

The instrument IKS and its calibration

The IKS infrared spectro-photometer will fly on board the VEGA platforms. It is designed to characterize the size, temperature and emissivity of the Comet Halley nucleus, to identify the major gaseous components of the inner coma and to detect the emission of the cometary grains. This paper presents the “calibration” experiments required to reduce the raw data: (i) absolute wavelength calibration of the filter wheels; (ii) modeling of the internal signal, as a function of the temperature of the different sub-systems; (iii) absolute and spectral responsivities of each of the spectrometric and photometric channels, as a function of the wavelength and position of the source in the field of view. Finally, we shall indicate the expected S/N ratios.