Molecular analysis of the graviperception signal transduction in the flagellate Euglena gracilis: Involvement of a transient receptor potential-like channel and a calmodulin

Euglena gracilis, a unicellular, photosynthetic flagellate is a model system for environmentally controlled behavior responses. The organism shows pronounced negative gravitaxis. This movement is based on physiological mechanisms, which in the past had been only indirectly assessed. It was shown that mechano-sensitive calcium channels are involved in the gravitaxis response. Recent studies have demonstrated that members of the transient receptor potential (TRP) family function as mechano-sensitive channels in several different cell types. We have sequenced part of a TRP gene in Euglena and applied RNA interference (RNAi) to confirm that these channels are involved in graviperception. It was found that RNAi against the putative TRP channel abolished gravitaxis. The genes of three calmodulins were sequences in Euglena, one of which was previously known in its protein structure (cal 1). The other two were unknown (cal 2 and cal 3). Cal 2 has been analyzed in detail. The biosynthesis of the corresponding proteins of cal 1 and cal 2 was inhibited by means of RNA interference to see whether this blockage impairs gravitaxis. RNAi of cal 1 leads to a long-term loss of free swimming in the cells (while euglenoid movement persists). It induced pronounced cell form aberrations and the division of cells was hampered. After recovery from RNAi the cell showed precise negative gravitaxis again. Thus cal 1 does not seem to be involved in gravitaxis. In contrast, the blockage of cal 2 has no pronounced influence on motility and cell form but leads to a complete loss of gravitactic orientation for more than 30 days showing that this calmodulin is an element in the signal transduction chain. The data are discussed in the context of the current model of the gravitaxis signal transduction chain in Euglena gracilis.     

On maximum luminosities of X-ray bursts

In this paper we address problems associated with the observation of super-Eddington luminosities in X-ray burst sources and discuss some possible solutions.     

THE SIGNAL OF THE 11-YEAR SUNSPOT CYCLE IN THE UPPER TROPOSPHERE-LOWER STRATOSPHERE

The paper summarizes work by the authors over the past ten years on an apparent signal of the 11-year sunspot cycle in the lower stratosphere-upper troposphere. The signal appears as a basic, consistent pattern in correlations between heights of stratospheric constant-pressure levels, at least as high as 25 km, and the solar cycle in which the highest correlations are in the subtropics.The variation of the stratospheric heights in phase with the sunspot cycle are – in the areas of high correlations between the two – associated with temperature variations on the same time scale in the middle and upper troposphere. The spatial distribution of the correlations suggests that the year-to-year changes in tropical and subtropical vertical motions contain a component on the time scale of the solar cycle.In January and February the correlations with the sunspot cycle are smallest. The smallness of the correlations is owing to the fact that they are different in the east and west years of the quasi-biennial oscillation in the equatorial stratospheric winds. The correlation pattern in the east years is the same as in the other seasons and is statistically significant. In the west years the correlations are insignificant outside the arctic, and the positive correlation in the arctic in these years is related to the fact that major midwinter breakdowns of the cyclonic vortex in the west years so far have happened only at maxima in the solar cycle.Until recently reliable continuous series of analyses of the stratosphere were not available for the southern hemisphere. The U.S. National Centers for Environmental Prediction and the National Center for Atmospheric Research have now, however, issued a 23-year series of re-analyzed global data which has made it possible to detect the solar signal on the southern hemisphere. It turns out to be almost the same as that on the northern hemisphere.The correlations between total column ozone and the sunspot cycle are lowest in the equatorial regions, where ozone is produced, and in the subpolar regions, where the largest amounts are found. In the annual mean the largest correlations lie between 5° lat. and 30° lat. We suggest that this distribution of correlations is due to the fact that the subtropical heights of the constant-pressure surfaces in the ozone layer are higher in maximum than in minimum years of the sunspot cycle, and that the higher subtropical heights in the solar maxima depress the poleward transport of ozone through the subtropics and thus create an abundance of ozone.     

Ukrainian national space law from an international perspective

As one of the three former Soviet republics engaged in space activities, the Ukraine has had to formulate new national space legislation as a means of demonstrating a responsible attitude to the international security system, of harmonising its legislation with that of its international political and economic partners and of creating clear guidelines for investors. This article presents the background to the formation of Ukrainian space law, describes some of the new laws enacted and discusses these within the context of international space law. Particular attention is paid to the legal regulation of commercial activities, to dual-use issues and to the effect of international cooperation on Ukrainian space law development.     

Results of the parabolic flight tests of the rapunzel deployer

The tether assisted re-entry of small payloads is a highly interesting tool for space transportation especially for the return of small payloads from Space Station ISSA. The small tether mission Rapunzel was initiated in 1991 by the Institute of Astronautics, TU München and the Kayser-Threde Company, to design a low cost and feasible tether experiment for the verification of the tether assisted re-entry. Together with the Samara State Aerospace University, Russia, a mission concept on a Russian Resurs or Photon capsule was developed. Based on this mission a deployer has been designed, mainly based on technology of the textile industry, which insures high reliability at low cost. Recently a similar configuration is being discussed for the ESA-TSE mission.The main work during the recent time was the development and test of the breadboard model of the deployer system. After successfully completing initial ground tests with the deployer, further tests during the ESA Parabolic Flight campaign in November 1995 were conducted. After a short introduction of the overall mission scenario, the planned configuration in orbit, this paper will present the results of the microgravity test campaign onboard the KC-135 aircraft and compare them with the ground test. The deployer showed a good performance during all tests, including ejection of the end-mass, deployment, and braking. Problems that occurred during the tests will be discussed, and solutions for the detected flaws and the results of the redesign now in progress will be presented. These verifications have shown the feasibility of the concept and will lay the base for the planned development of the flight model of the deployer.     

Evolution of X-ray binaries: Achievements and advances

Three recent developments in the field of formation and evolution of neutron stars and black holes in binaries are addressed:

• The finding that there is a class of neutron stars, formed in interacting binaries, that do not receive kick velocities in their birth events. This finding is particularly important for our understanding of the formation – and formation rates – of double neutron stars. It is argued that these low-kick neutron stars, which tend to have low masses, are formed by a different physical mechanism than the neutron stars that receive large kick velocities at birth.

• The occurrence of velocity kicks in the formation events of stellar black holes.

• The nature of the companions of millisecond X-ray pulsars.

Studying the evolution of the hot universe with the X-ray evolving universe spectroscopy mission – XEUS

Europe is one of the major partners building the International Space Station (ISS) and European industry, together with ESA, is responsible for many station components including the Columbus Orbital Facility, the Automated Transport Vehicle, two connecting modules and the European Robotic Arm. Together with this impressive list of contributions there is a strong desire within the ESA Member States to benefit from this investment by utilizing the unique capabilities of the ISS to perform world-class science. XEUS is one of the astronomical applications being studied by ESA to utilize the capabilities of the ISS. XEUS will be a long-term X-ray observatory with an initial mirror area of 6 m² at 1 keV that will be expanded to 30 m² following a visit to the ISS. The 1 keV spatial resolution is expected to be 2–5″ half-energy-width. XEUS will consist of separate detector and mirror spacecraft (MSC) aligned by active control to provide a focal length of 50 m. A new detector spacecraft, complete with the next generation of instruments, will also be added after visiting the ISS. The limiting 0.1–2.5 keV sensitivity will then be 4 × 10⁻¹⁸ erg cm⁻² s⁻¹, around 200 times better than XMM-Newton, allowing XEUS to study the properties of the hot baryons and dark matter at high redshift.