Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

The observation of the galactic anticenter region by the balloon borne gamma-ray telescope Natalya-I

This paper presents the results of measurements of gamma radiation with energies above 5 MeV, from the galactic anticenter region. The balloon-borne gamma ray telescope “Natalya-I”, was launched on 6 November, 1980 from the Tata Institute of Fundamental Research's Balloon Facility (Hyderabad, India) and reached ceiling altitude of 35 km. The results on the accelerator calibration of the telescope, using a “tagged” gamma ray beam are also presented.     

Implementation of a new characteristic parameter into the IRI sub-peak electron density profile

The shape of electron density profile in the International Reference Ionosphere could be improved significantly if the height h_g and electron density N_g of the F region sub-peak inflexion point were entered in the set of the profile standard parameters. To study variations of these important parameters, the N(h) analysis of the statistically-summarized ionograms at the latitudes of 40–80°N of the Eastern hemisphere has been carried out for the two-hours intervals of local time, three seasons (winter, equinox and summer) and two levels of solar activities characterized by Covington indicesF_10.7 = 100 and 200. It is shown that the parameters of the inflexion point can be expressed in most cases via the peak parameters of the F2 layer ash_g= 0.8 hmF2 and N_g= 0.5 NmF2.     

Satellite observations of upper atmosphere O3 and HNO3 from the Limb Infrared Monitor of the Stratosphere (LIMS) experiment on Nimbus 7

The Limb Infrared Monitor of the Stratosphere (LIMS) experiment is a limb scanning infrared sounder designed to measure vertical temperature profiles and the concentrations of key chemical compounds which are important in the stratospheric ozone-nitrogen photochemistry. This paper describes results from the O₃ and HNO₃ channels with emphasis on validation of the data. Similar discussions of results from the other channels are presented in two companion papers published in these proceedings.     

FIGARO: An experiment for pulsar and variable source studies in the MeV range

We present a large area, balloon borne, NaI(Tl) detector for low-energy gamma rays with temporal signature : FIGARO.The main detector is a mosaic of 12 NaI(Tl) tiles 22.5 × 15 × 5 cm, for a total geometric area of 4050 cm².In the energy band 140 keV - 6 MeV, the expected background counting rate at float altitude is in the range of two to three thousands counts per second.For pulsar analysis the expected 3δ sensitivity for 5 hours exposition time is 2.5 10^?4 ph/cm².s.MeV (150–500 keV) 1.5 10^?4 ph/cm².s.MeV (1–6 MeV). This performance, together with the large effective area and the relatively short duration of a balloon flight, make FIGARO particularly suitable for the identification of sources by means of temporal analysis.For objectives in the Northern sky, including the Crab pulsar, a transmediterranean flight is planned for the summer of 1982 ; a Southern mission is scheduled in Brazil for the fall of 1983 (Vela, PSR 1822-09).     

Initial results from COMPTEL onboard GRO

COMPTEL is the first imaging telescope to explore the MeV gamma-ray range (0.7 to 30 MeV). At present, it is performing a complete sky survey. In later phases of the mission selected celestial objects will be studied in more detail. The data from the first year of the mission have demonstrated that COMPTEL performs very well. First sky maps of the inner part of the Galaxy clearly identify the plane as a bright MeV-source (probably due to discrete sources as well as diffuse radiation). The Crab and Vela pulsar lightcurves have been measured with unprecedented accuracy. The quasars 3C273 and 3C279 have been seen for the first time at MeV energies. Both quasars show a break in their energy spectra in the COMPTEL energy range. The 1.8 MeV line from radioactive ²⁶A1 has been detected from the central region of the Galaxy and a first sky map of the inner part of the Galaxy has been obtained in the light of this line. Upper limits to gamma-ray line emission at 847 keV and 1.238 MeV from SN 1991T have been derived. Upper limits to the interstellar gamma-ray emissivity have been determined at MeV-energies. Several cosmic gamma-ray bursts within the field-of-view have been located with an accuracy of about 1°. On 1991 June 9, 11 and 15, COMPTEL observed gamma-ray emission (continuum and line) from three solar flares. Also neutrons were detected from the June 9 and June 15 flares.     

Pulsar studies with GRO-COMPTEL

Pulsar measurements performed by the experiment COMPTEL, aboard the Compton Gamma Ray Observatory, are described. The main results refer to the Crab and Vela pulsars whose pulse shape characteristics are given in some detail and light curves are compared with those above 50 MeV, as observed by the COS-B satellite. No other gamma-ray pulsars have been detected to date by COMPTEL, the upper limit on the pulsed signal from Geminga being compatible with indications by other experiments.     

Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems.

Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. Grant numbers: 99-04-96017, 00-07-9011.     

Transformation of a delta: Converting estuaries to lakes

In this century large-scale transformations have been carried out in the delta of the Rhine, Meuse and Scheldt in The Netherlands. The following principal consequences are considered: the loss of estuarine ecosystems, the change in the process of sedimentation and erosion, and the development of new aquatic and terrestrial ecosystems. The ecological consequences of the transformation of the Grevelingen estuary into a salt-water lake and some surprising developments are described briefly. The need for a limited restoration of the water exchange between the lake and the North Sea is indicated, as well as the major impacts of the exchange. The necessity for the maintenance or restoration of estuaries, the ecological development and the possibilities of the new lakes, and the management of these lakes by engineering structures are discussed. Careful land-use planning and ecosystem management, backed by research, are needed to make the fullest possible use of the potential offered by new lakes. It is also concluded that salt lakes have interesting perspectives.     

The structural features of the germanium-silicon solid solution crystals obtained under microgravity

Structural features of germanium-silicon solid solution crystals have been investigated and silicon distribution in the crystals has been studied. All the crystals obtained under microgravity had, in spite of good external shape and facetting, a poorer internal structure than those obtained on Earth. The distribution of silicon have been shown to be non-uniform. High dislocation densities were observed.