Effects of spaceflight (STS-87) on tropisms and plastid positioning in protonemata of the moss Ceratodon purpureus.

Apical cells of moss protonemata represent a single-celled system that perceives and reacts to light (positive and negative phototropism) and to gravity (negative gravitropism). Phototropism completely overrides gravitropism when apical cells are laterally irradiated with relatively high red light intensities, but below a defined light intensity threshold gravitropism competes with the phototropic reaction. A 16 day-long exposure to microgravity conditions demonstrated that gravitropism is allowed when protonemata are laterally illuminated with light intensities below 140 nmol m-2s-1. Protonemata that were grown in darkness in microgravity expressed an endogenous tendency to grow in arcs so that the overall culture morphology resembled a clockwise spiral. However this phenomenon only was observed in cultures that had reached a critical age and/or size. Organelle positioning in dark-grown apical cells was significantly altered in microgravity. Gravisensing most likely involves the sedimentation of starch-filled amyloplasts in a well-defined area of the tip cell. Amyloplasts that at 1-g are sedimented were clustered at the apical part of the sedimentation zone in microgravity. Clustering observed in microgravity or during clino-rotation significantly differs from sedimentation-induced plastid aggregations after inversion of tip cells at 1-g.     

The influence of dose, dose-rate and particle fragmentation on cataract induction by energetic iron ions.

Because activities in space necessarily involve chronic exposure to a heterogeneous charged particle radiation field it is important to assess the influence of dose-rate and the possible modulating role of heavy particle fragmentation on biological systems. Using the well-studied cataract model, mice were exposed to plateau 600 MeV/amu 56Fe ions either as acute or fractionated exposures at total doses of 5 - 504 cGy. Additional groups of mice received 20, 360 and 504 cGy behind 50 mm of polyethylene, which simulates body shielding. The reference radiation consisted of 60Co gamma radiation. The animals were examined by slit lamp biomicroscopy over their three year life spans. In accordance with our previous observations with heavy particles, the cataractogenic potential of the 600 MeV/amu 56Fe ions was greater than for low-LET radiation and increased with decreasing dose relative to gamma-rays. Fractionation of a given dose of 56Fe ions did not reduce the cataractogenicity of the radiation compared to the acute regimen. Fragmentation of the beam in the polyethylene did not alter the cataractotoxicity of the ions, either when administered singly or in fractions.     

Lower and upper limits to comet p/halley solid material loss rate

A radiative hydrodynamic model of comet P/Halley is used to investigate quantitatively the constraints imposed onto the Comet loss rate in solids by both the in-situ data and the remote sensing data. In particular, for the first time, the in-situ data are used to fit composite spectra extending from near-IR wavelengths to the microwave region. Also, for the first time the uncertainty affecting the ejection velocity of large grains is taken into account. The results suggest that only future rendez-vous studies of the grain density in the vicinity of a comet would be able to provide definitely reliable values of the comet loss rate.     

A bioreactor system for the nitrogen loop in a Controlled Ecological Life Support System.

As space missions become longer in duration, the need to recycle waste into useful compounds rises dramatically. This problem can be addressed by the development of Controlled Ecological Life Support Systems (CELSS) (i.e., Engineered Closed/Controlled Eco-Systems (ECCES)), consisting of human and plant modules. One of the waste streams leaving the human module is urine. In addition to the reclamation of water from urine, recovery of the nitrogen is important because it is an essential nutrient for the plant module. A 3-step biological process for the recycling of nitrogenous waste (urea) is proposed. A packed-bed bioreactor system for this purpose was modeled, and the issues of reaction step segregation, reactor type and volume, support particle size, and pressure drop were addressed. Based on minimization of volume, a bioreactor system consisting of a plug flow immobilized urease reactor, a completely mixed flow immobilized cell reactor to convert ammonia to nitrite, and a plug flow immobilized cell reactor to produce nitrate from nitrite is recommended. It is apparent that this 3-step bioprocess meets the requirements for space applications.     

New instrumentation for optical measuring of oxygen in gas or dissolved in liquids.

The optical oxygen sensor is a novel device for the determination of oxygen in gases or dissolved in liquids. It is based on the measurement principle of fluorescence quenching, which is completely different from that of polarographic oxygen sensors (today the most widespread devices of oxygen detection). The new instrument offers features and advantages, which render it not only a realistic alternative, but, for specific applications, make it superior to existing electrochemical methods. The system is based on low-cost semiconductor devices (light-emitting diodes, photodiodes, low-cost analogue and digital components) and new LED-compatible oxygen-sensitive membranes. The flow cell of the instrument may be thermostatted and the sensor can be calibrated by a simple two-point calibration procedure. The optical oxygen sensor is particularly suitable for measuring dissolved oxygen in respirometry, since no oxygen is consumed by the device and the signal is independent of sample flowrate or stirring speed. Typical fields of application are monitoring of oxygen in ground and drinking water, in process control in bioreactors and in breath gas and blood gas analysis.     

Analysis of radiation risk from alpha particle component of solar particle events.

The solar particle events (SPE) will contain a primary alpha particle component, representing a possible increase in the potential risk to astronauts during an SPE over the often studied proton component. We discuss the physical interactions of alpha particles important in describing the transport of these particles through spacecraft and body shielding. Models of light ion reactions are presented and their effects on energy and linear energy transfer (LET) spectra in shielding discussed. We present predictions of particle spectra, dose, and dose equivalent in organs of interest for SPE spectra typical of those occurring in recent solar cycles. The large events of solar cycle 19 are found to have substantial increase in biological risk from alpha particles, including a large increase in secondary neutron production from alpha particle breakup.     

MEMS Electronically Steerable Antennas for Fire Control Radars

Large apertures are of great benefit to applications that are prime powered limited as is found on aerostat and other airborne platforms. Electronically scanned array antennas are often proposed for these applications. However, increasing the aperture area with conventional array technology is met with prohibitive cost, weight, and prime power increases because of the dense spacing of phase shifters and/or active T/R modules. This discusses the recent development of RF MEMS (Microelectromechanical System) switch technology and the use of these switches in a Radanttrade lens configuration for arrays of approximately 10 m² at X-band. A proof-of-concept 0.4 m² MEMS Electronically Steerable Antenna (ESA) containing 25,000 MEMS switches has been successfully designed, fabricated, and tested. The 0.4 m² MEMS ESA was then integrated with an AN/APG-67 radar system to form the MEMS Demonstration Radar System. The MEMS Demonstration Radar System successfully detected both airborne and ground moving targets during a series of extensive radar demonstrations. This is believed to be the first large scale employment of MEMS switches in a scanning antenna and radar system. The low-cost, lightweight, and low power technology demonstrated can enable weight and power constrained platforms with electronic steering.     

Distance estimation at 60 GHz

An FM-CW radar front-end was fabricated in an integrated manner at 60 GHz by using the NRD guide. Main emphasis was placed on compactness in size and high-precise operation in performance. The fabricated radar consists of an FM Gunn oscillator, a balanced mixer, and a planar antenna fed by leaky NRD guide with a mechanically beam-scanning performance. All circuit components and the antenna were contained in a compact housing of 170 /spl times/ 140 mm in area and 25 mm in thickness, and thus, a thin type of millimeter-wave radar front-end was successfully developed. Moreover, an error of distance estimation was measured to be less than a distance of 0.7 m.     

Operational testing of valve regulated lead acid batteries incommercial aircraft

Valve regulated lead acid (VRLA) batteries provide electrical performance that is virtually identical to sintered plate nickel-cadmium battery systems. In addition, the VRLA batteries offer the user a no-maintenance battery and other enhanced features that make this a very desirable battery for aircraft applications. In field trials, where VRLA batteries were substituted for nickel-cadmium batteries, the VRLA provided the user with a high reliability turbine engine starting battery under a wide variety of climatic conditions     

Nondestructive Characterization of RBSOA of High-Power Bipolar Transistors

Reverse-bias safe operating area (RBSOA) of high-power Darlington transistors is characterized using a 120 A/1000 V nondestructive reverse-bias second breakdown tester designed and fabricated at Virginia Polytechnic Institute and State University. Elaborate RBSOA characteristics are generated with different forward/reverse base drives and collector current levels. The effects of elevated case temperature and second-base drive on RBSOA of four-terminal Darlington devices are also discussed.