Bacterial community in ancient Siberian permafrost as characterized by culture and culture-independent methods

The microbial composition of ancient permafrost sediments from the Kolyma lowland of Northeast Eurasia was examined through culture and culture-independent approaches. These sediments have been continuously frozen for 5,000 to 2-3 million years. A total of 265 Bacteria 16S rRNA gene sequences were amplified from the permafrost total-community genomic DNA and screened by amplified ribosomal 16S rRNA restriction analysis. Members of three major lineages were found: gamma-Proteobacteria (mostly Xanthomonadaceae), Actinobacteria, and Firmicutes. We also determined partial 16S rRNA gene sequences of 49 isolates from a collection of 462 aerobes isolated from these sediments. The bacteria included Actinomycetales (Arthrobacter and Microbacteriaceae); followed by the Firmicutes (Exiguobacterium and Planomicrobium); the Bacteroidetes (Flavobacterium); the gamma-Proteobacteria (Psychrobacter); and the alpha-Proteobacteria (Sphingomonas). Both culture and culture-independent approaches showed the presence of high and low G+C Gram-positive bacteria and gamma-Proteobacteria. Some of the 16S rRNA gene sequences of environmental clones matched those of Arthrobacter isolates. Two-thirds of the isolates grew at -2.5 degrees C, indicating that they are psychroactive, and all are closely related to phylogenetic groups with strains from other cold environments, mostly commonly from Antarctica. The culturable and non-culturable microorganisms found in the terrestrial permafrost provide a prototype for possible life on the cryogenic planets of the Solar System.     

Ultrastructural study of iron oxide precipitates: implications for the search for biosignatures in the Meridiani hematite concretions, Mars

Two terrestrial environments that have been proposed as analogs for the iron oxide precipitation in the Meridiani Planum region of Mars include the Rio Tinto precipitates and southern Utah marble concretions. Samples of two typical Utah iron oxide concretions and iron oxide precipitates in contact with biofilms from Rio Tinto have been studied to determine whether evidence could be found for biomediation in the precipitation process and to identify likely locations for fossil microorganisms. Scanning electron microscopy, energy dispersive X-ray, and gas chromatography-mass spectrometry (GC-MS) were used to search for biosignatures in the Utah marbles. The precipitation of iron oxides resembles known biosignatures, though organic compounds could not be confirmed with GC-MS analysis. In contrast, textural variations induced by biological activity are abundant in the modern Rio Tinto samples. Although no compelling evidence of direct or indirect biomediation was found in the Utah marbles, the ultrastructure of the iron oxide cement in the concretion suggests an inward growth during concretion precipitation from an initially spherical redox front. No indication for growth from a physical nucleus was found.     

Entrapment of bacteria in fluid inclusions in laboratory-grown halite

Cells of the bacterium Pseudomonas aeruginosa, which were genetically modified to produce green fluorescent protein, were entrapped in fluid inclusions in laboratory-grown halite. The bacteria were used to inoculate NaCl-saturated aqueous solutions, which were allowed to evaporate and precipitate halite. The number, size, and distribution of fluid inclusions were highly variable, but did not appear to be affected by the presence of the bacteria. Many of the inclusions in crystals from inoculated solutions contained cells in populations ranging from two to 20. Microbial attachment to crystal surfaces was neither evident nor necessary for entrapment. Cells occurred exclusively within fluid inclusions and were not present in the crystal matrix. In both the inclusions and the hypersaline solution, the cells fluoresced and twitched, which indicates that the bacteria might have remained viable after entrapment. The fluorescence continued up to 13 months after entrapment, which indicates that little degradation of the bacteria occurred over that time interval. The entrapment, fluorescence, and preservation of cells were independent of the volume of hypersaline solution used or whether the solutions were completely evaporated prior to crystal extraction. The results of this study have a wide range of implications for the long-term survival of microorganisms in fluid inclusions and their detection through petrography. The results also demonstrate the preservation potential for microbes in hypersaline fluid inclusions, which could allow cells to survive harsh conditions of space, the deep geologic past, or burial in sedimentary basins.     

TUGSAT-1/BRITE-Austria—The first Austrian nanosatellite

A nanosatellite to investigate the brightness oscillations of massive luminous stars by differential photometry is currently developed by a Canadian/Austrian team within the BRITE (Bright Target Explorer) project. The first Austrian satellite funded by the Austrian Space Program, called TUGSAT-1/BRITE-Austria, builds on the space heritage of the most successful Canadian CanX-2 and MOST missions. The satellite makes use of recent advances in miniaturized attitude determination and control systems. Precision three-axis stabilization by small reaction wheels and a star tracker provides the necessary accuracy for the photometer telescope to the arcminute level. This will provide to the astronomers photometric data of the most massive stars with unprecedented precision; data which cannot be obtained from the ground due to limitations imposed by the terrestrial atmosphere.The paper describes the spacecraft characteristics and the ground infrastructure being established in support of the BRITE mission which will consist of a constellation of up to four nearly identical satellites allowing to carry out long-term observation of stars (magnitude +3.5) not only with respect to brightness variations, but also in different spectrum ranges.     

Estimation of dynamic characteristics of the <Emphasis Type="Italic">International Space Station</Emphasis> from measurements of microaccelerations

We describe the results of determining the mass of the International Space Station using the data of MAMS accelerometer taken during correction of the station orbit on August 20, 2004. The correction was made by approach and attitude control engines (ACE) of the Progress transporting spacecraft. The engines were preliminary calibrated in an autonomous flight using the onboard device for measuring apparent velocity increment. The method of calibration is described and its results are presented. The error in station mass determination is about 1%. The same data of MAMS and similar data obtained during the orbit correction on August 26, 2004 were used for the analysis of high-frequency vibrations of the station mainframe caused by operation of the ACE of Progress. Natural frequencies of the ACE are determined. They lie in the frequency band 0.024–0.11 Hz. ACE operation is demonstrated to result in a substantial increase of microaccelerations onboard the station in the frequency range 0–1 Hz. The frequencies are indicated at which disturbances increase by more than an order of magnitude. The study described was carried out as a part of the Tensor technological experiment.     

Microperturbations on the <Emphasis Type=Italic>International Space Station</Emphasis> during physical exercises of the crew

The results of a preliminary analysis of microperturbations on the International Space Station during physical exercises of the crew are presented. The goal of this paper is to identify the parameters of perturbations when physical exercises are performed. The results of measurements by sensors of microaccelerations of both Russian and American segments during physical exercises in the service module of the Russian segment are analyzed.     

Erratum to: Postvolcanic Aerosols: Measurements of Altitude and Particle Sizes with Twilight-Sky Polarimetry

Cosmic Research - An Erratum to this paper has been published: https://doi.org/10.1134/S0010952521110010     

Doppler characteristics of HF spread Doppler clutter recorded at Alice Springs,Australia

Mean night-time peak power, Doppler shift and Doppler width of spread Doppler clutter (SDC) received by a high frequency backscatter radar located at Alice Springs, Australia from 2000 to 2018 is presented as a function of azimuth, sunspot number, time of year and frequency. The sampled region covers 90 degrees from West to North and includes the northern and southern equatorial anomalies.SDC peak power diminished across all azimuths during the winter solstice from around May to August (local winter) coinciding with the global decrease in F layer density due to the annual non-seasonal F2 anomaly but was generally constant during the equinoxes. In contrast, SDC Doppler width and inbound Doppler shift both increased during the equinoxes and exhibited azimuthal dependence related to the eastward equatorial plasma drift.SDC peak power increased with increasing sunspot number with frequency dependence during winter but not summer. Inbound Doppler shift and Doppler width increased with increasing sunspot number during equinox but not solstice with a strong dependence on azimuth and a weak dependence on frequency.     

A novel satellite image encryption algorithm based on hyperchaotic systems and Josephus problem

With the rapid growth of the number of Earth observation satellite (EOS) supporting critical applications, it is required to improve the security techniques to protect the sensitive data and images during the transmission between the satellites and the ground stations. This paper introduces a new satellite image encryption algorithm based on the Linear Feedback Shift Register (LFSR) generator, SHA 512 hash function, hyperchaotic systems, and Josephus problem. LFSR generates a matrix that is used to construct the 512-bits value of the hash function. These bits are used to set the initial values and parameters of the proposed encryption algorithm. Firstly, the six dimensions (6-D) hyperchaotic system is divided into three parts, where every two equations are considered as one part. Secondly, the 1-D hyperchaotic logistic-tent system is considered as the controller to select one part. The selected part is used to generate a matrix that is XORed with the original image. Thirdly, the scrambling operation by Josephus sequences is applied to the output of the previous step by scrambling the rows and the columns according to the selected part to produce the pre-encrypted image. Finally, if the number of iterations is less than the required number which is considered as a parameter of the secret key, the previous operations will be repeated in the pre-encrypted image; otherwise, the pre-encrypted image is considered as the final cipher image. Experimental and analyses results show that the proposed algorithm has good performance in terms of high level of security, large enough key-space, tolerance to Single Event Upsets (SEU) as well as low time complexity.     

Confirmation of gravisensitivity in the slime mold physarum polycephalum under near weightlessness

We have investigated Physarum polycephalum, a unicellular organism with no special gravity receptors, on its ability to react to gravity. The first experiments were 0 g-simulation experiments on the fast-rotating clinostat conducted with plasmodial strands of this acellular slime mold. In these earth-bound experiments the observed parameters were periodicity of the contractions and dilatations of the strand's ectoplasm as well as the periodicity and velocity of the striking cytoplasmic (endoplasmic) shuttle streaming. During 0 g-simulation these parameters showed significant changes indicating the existence of a gravisensitivity of the slime mold.

The Space-Shuttle experiment (ESA-Biorack in D 1-Mission) should demonstrate the validity of the 0 g-simulation on the fast-rotating clinostat. The experiment was designed in a way enabling the registration of the same parameters as on the clinostat (using the light microscope in combination with a photo diode and a cinecamera). Only one of the two planned measurement sessions was fully successful and provided us with data confirming the results gained on the fast-rotating clinostat: The slime mold showed under real near weightlessness in the D 1-Space Shuttle Mission a transient frequency increase in its contraction rhythmicity and a (steady) increase in the streaming velocity of its endoplasm.