Loading effects on rat craniomandibular morphology: a system for gravity studies

Gravity effects on muscle and bone are a major impediment to long-term space travel. We introduce a model for studying these effects, the craniomandibular system. Some advantages of this system include: (1) craniomandibular morphology is determined by epigenetic factors including gravity, (2) relatively light forces can significantly alter its morphology, and (3) soft diet and tooth loss produce effects that are similar to those produced in lower limbs by weightlessness. In the study, implants made either of gold (experimental group) or lightweight acrylic (controls) were attached to adult rats' mandibles. After 13 weeks, the animals' skulls and mandibles were dissected. Pair-wise comparisons indicated that the experimental animals showed significantly shortened and narrowed cranial bases, and significant changes in the posterior zygomatic arch region. These results indicate that simulated macrogravity influences bone remodeling in the adult craniomandibular system.     

Plasma irregularities in unstable outer ionosphere according to the Intercosmos-Bulgaria-1300 satellite data

The results of the satellite low-latitude and mid-latitude measurements of the disturbed plasma concentration, electron temperature, and quasi-stable electric field at heights of ～900 km after sunset are discussed. It is shown that the sharp fronts of changes in the electron temperature and plasma density observed in the experiment onboard the Intercosmos-Bulgaria-1300 satellite in the low-latitude (and equatorial) outer ionosphere can be related to damping of the oscillations of plasma electrons at local decreases of the plasma density (plasma “pits”) and formation of the vortex plasma structures at density and temperature gradients, which promotes conservation of ionosphere irregularities and makes the fronts of concentration variations steeper. Nonmonotonic variations in the plasma conductivity for the ionosphere currents in unstable plasma can be a cause of observed nonmonotonic disturbances of the vertical component of the “constant” electric field.     

Detection of ultrahigh-energy cosmic rays and neutrinos by radio method using artificial lunar satellites

An estimate of the feasibility of the ultrahigh-energy cosmic ray and neutrino detection using a lunar satellite-borne radio receiver is presented. The data obtained in the proposed experiment will make resolving the current contradictions in the ultrahigh-energy cosmic ray spectra measured with the major ground-based instruments possible. Moreover, they will enable us to considerably extend the accessible energy range and to check predictions of various models of the origin of the highest-energy particles in the Universe. At the same time the lunar radio detector provides a means of searching for ultrahigh-energy neutrinos with a high sensitivity combined with a very large target effective mass.     

Numerical simulation of effects of the August 11, 1999 solar eclipse in the outer ionosphere

The results of a numerical simulation of such parameters of the topside ionosphere as concentration N _e and temperature T _e of electrons, and concentration n(H⁺) and fluxes along the magnetic field lines Φ(H⁺) of H⁺ ions at an altitude of ～2000 km for the conditions of the August 11, 1999 solar eclipse are presented. The calculations were performed using the Global Self-consistent Model of the Thermosphere, Ionosphere, and Protonosphere of the Earth (GSM TIP). It is shown that during the eclipse, in addition to a region of decreased values of T _e in the Northern Hemisphere and in the magnetically conjugate region in the Southern Hemisphere, regions of electron heating emerge in both hemispheres. Simultaneously, an extended region of decreased values of N _e comes into existence and moves behind the Moon’s shadow. Regions with decreased (down to ～30%) and enhanced (up to ～50%) concentrations of H⁺ ions are detected in the global distribution of these ions.     

Spaceflight experiments with Arabidopsis starch-deficient mutants support a statolith-based model for graviperception.

In order to help resolve some of the controversy associated with ground-based research that has supported the starch-statolith theory of gravity perception in plants, we performed spaceflight experiments with Arabidopsis in Biorack during the January 1997 and May 1997 missions of the Space Shuttle. Seedlings of wild-type (WT) Arabidopsis, two reduced-starch strains, and a starchless mutant were grown in microgravity and then were given either a 30, 60, or 90 minute gravity stimulus on a centrifuge. By the 90 min 1-g stimulus, the WT exhibited the greatest magnitude of curvature and the starchless mutant exhibited the smallest curvature while the two reduced starch mutants had an intermediate magnitude of curvature. In addition, space-grown plants had two structural features that distinguished them from the controls: a greater number of root hairs and an anomalous hypocotyl hook structure. However, the morphological changes observed in the flight seedlings are likely to be due to the effects of ethylene present in the spacecraft. (Additional ground-based studies demonstrated that this level of ethylene did not significantly affect gravitropism nor did it affect the relative gravitropic sensitivity among the four strains.) Nevertheless, this experiment on gravitropism was performed the "right way" in that brief gravitational stimuli were provided, and the seedlings were allowed to express the response without further gravity stimuli. Our spaceflight results support previous ground-based studies of these and other mutants since increasing amounts of starch correlated positively with increasing sensitivity to gravity.     

Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.

The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.     

New insights into Titan's organic chemistry in the gas and aerosol phases.

Titan, the largest satellite of Saturn, with a dense atmosphere very rich in organics, and many couplings in the various parts of its "geofluid", is a reference for studying prebiotic chemistry on a planetary scale. New data have been obtained from experiments simulating this organic chemistry (gas and aerosol phases), within the right ranges of temperature and a careful avoiding of any chemical contamination. They show a very good agreement with the observational data, demonstrating for the first time the formation of all the organic species already detected in Titan atmosphere including, at last, C4N2, together with many other species not yet detected in Titan. This strongly suggests the presence of more complex organics in Titan's atmosphere and surface, including high molecular weight polyynes and cyanopolyynes. The NASA-ESA Cassini-Huygens mission has been successfully launched in October 1997. The Cassini spacecraft will reach the Saturn system in 2004 and become an orbiter around Saturn, while the Huygens probe will penetrate into Titan's atmosphere. In situ measurements, in particular from Huygens GC-MS and ACP instruments, will provide a detailed analysis of the organics present in the air, aerosols, and surface. This very ambitious mission should yield much information of crucial importance for our knowledge of the complexity of Titan's chemistry, and, more generally for the field of exobiology.     

Application of the graph-analytical method of tree risk assessment to study survivability of small trust liquid rocket engines

The present paper discusses a need to develop a methodology of predicting the reliability of small thrust liquid rocket engines with a flow section made of composite materials under actual operating conditions for their successful practical use in the propulsion systems.     

Technique of integral diagnostics for a radio-frequency inductively coupled plasma discharge unit of an RF ion thruster

A technique of integral diagnostics for an RF inductively coupled plasma gas discharge unit of an ion thruster is proposed. This technique includes a priori measurements of antenna coil electrical parameters in free space and in assembled state as well as experimental determination of the antenna coil currents with and without discharge.