Free radicals induced in solid DNA by heavy ion bombardment.

Free radical formation after heavy-ion bombardment was studied in solid, polycristalline pellets of DNA-constituents by analysing the dose-yield curves and the spectra obtained by ESR-spectroscopy at low (< 100 K) and ambient temperatures. The dose-yield curves were found to correlate with those found after X-irradiation but shifted to higher doses and lower saturation concentrations. The corresponding radical yields (per 100 eV) exhibit values which are one to two orders of magnitudes lower. The structural aspects as revealed from powder ESR-spectra gave a complex inter-relation between substance, LET, dose and irradiation temperature, which is discussed in terms of mechanistic models.     

Space telescope studies of Jupiter and Saturn simulated by Voyager observations

Space Telescope (ST) observations of Jupiter and Saturn will offer a unique opportunity for monitoring their changing meteorological characteristics. They will provide higher spatial and temporal resolution for composition and vertical structure studies than have been available to date. We have simulated the planetary camera observations of Jupiter and Saturn by Voyager images of the appropriate spatial scale. With this data set we have investigated the meteorological properties of these atmospheres which can be studied at these scales. In addition we have considered the advances obtainable with the high resolution spectrometer on ST compared with observations from ground-based and other Earth-orbiting satellites. These studies will provide insight into the scientific gain and possible problems in the use of ST for planetary studies.     

Assessing exposure to cosmic radiation on board aircraft.

The assessment of exposure to cosmic radiation on board aircraft is one of the preoccupations of organizations responsible for radiation protection. The cosmic radiation particle flux increases with altitude and latitude and depends on the solar activity. The radiation exposure has been estimated on several airlines using transatlantic, Siberian and transequatorial routes on board subsonic and supersonic aircraft, to illustrate the effect of these parameters. Measurements have been obtained with a tissue equivalent proportional counter using the microdosimetric technique. Data have been collected at maximum solar activity in 1991-92 and at minimum in 1996-98. The lowest mean dose rate measured was 3 microSv/h during a Paris-Buenos Aires flight in 1991; the highest was 6.6 microSv/h during a Paris-Tokyo flight using a Siberian route and 9.7 microSv/h on Concorde in 1996-97. The mean quality factor is around 1.8. The corresponding annual effective dose, based on 700 hours of flight for subsonic aircraft and 300 hours for Concorde, can be estimated between 2 mSv for least-exposed routes and 5 mSv for more exposed routes.     

Physiological, biochemical and molecular processes associated with gravitropism in roots of maize.

This research aims to characterize regulation of the principal cytosolic protein kinases in maize, cultivar 'Merit' root tips, since much evidence indicates that stimuli which modulate the gravitropic response in this system act through regulation of activity of these enzymes. To this end, we have cloned a maize protein kinase belonging to a group of plant protein kinases with a catalytic domain similar in primary structure to the second messenger-regulated protein kinases known in animal and fungal systems. However, both the unique structural features conserved among plant protein kinases in this group, and lack of evidence for cyclic nucleotide signalling in plants point to operation of a novel protein kinase regulatory mechanism in plants. In order to test effects of possible regulators on protein kinase activity, we developed a sensitive method for detecting regulation of autophosphoryl labelling of protein kinases in unfractionated maize protein extracts. Regulation of protein kinase autophosphorylation in these extracts was different from that known in animals and fungi, further suggesting operation of unique protein kinase regulatory mechanisms in plants. Previous research has shown that light, or factors modulated by light, regulate plant protein kinase activity. We found that protein kinase activity was co-immunoprecipitated with the plant photoreceptor phytochrome, and was associated with phytochrome by high-affinity chemical interactions. Far-red reversibility of red-light regulation of phytochrome phosphorylation by the associated protein kinase indicates that it may modulate or transduce the light signals which lead to gravitropic sensitivity in 'Merit' maize.     

Thermal cyclotron radiation from a hot coronal loop with helical magnetic field

The spectral and polarization properties of thermal cyclotron radio emission from a hot coronal loop with a current along the axis are computed. The magnetic field is supposed to have a component along the loop axis as well as a poloidal part due to the current, both components being of comparable magnitude. In this specific configuration a helical magnetic field is present with a remarkable minimum of its absolute value along the loop axis and a maximum at its periphery. The presence of one or two maxima of magnetic field value along the line of sight results in increasing optical thickness of the gyroresonance layers at appropriate frequencies in the microwave band and, therefore, in enhanced radio emission at those harmonics which are optically thin (for example,s=4). These cannot be observed in models with the commonly employed magnetic field configuration (longitudinal along the loop axis).We show that the frequency spectrum of thermal cyclotron radiation from a hot coronal loop with a helical magnetic field differs from that of the standards-component source (with smooth frequency characteristics and polarization corresponding toe-mode) in that plenty of fine structures (line-like features and cut-offs) are present and theo-mode is prevalent in some frequency intervals. The enhanced radio emission at high harmonics and the complicated form of frequency spectrum in the model considered imply that some microwave sources, which are poorly explained in traditional models of solar active regions, may be associated with helical magnetic fields in hot coronal loops. Computations allow one to indicate spectral and polarizational peculiarities of local sources testifying to the presence of a helical magnetic field.     

Performance and reliability of the NASA Biomass Production Chamber.

The Biomass Production Chamber (BPC) at the Kennedy Space Center is part of the Controlled Ecological Life Support System (CELSS) Breadboard Project. Plants are grown in a closed environment in an effort to quantify their contributions to the requirements for life support. Performance of this system is described. Also, in building this system, data from component and subsystem failures are being recorded. These data are used to identify problem areas in the design and implementation. The techniques used to measure the reliability will be useful in the design and construction of future CELSS. Possible methods for determining the reliability of a green plant, the primary component of a CELSS, are discussed.     

Galactic cosmic ray transport methods: past, present, and future.

The development of the theory of high charge and energy (HZE) ion transport is reviewed. The basic solution behavior and approximation techniques will be described. An overview of the HZE transport codes currently available at the Langley Research Center will be given. The near term goal of the Langley program is to produce a complete set of one-dimensional transport codes. The ultimate goal is to produce a set of complete three-dimensional codes which have been validated in the laboratory and can be applied in the engineering design environment. Recent progress toward completing these goals is discussed.     

Cognitive deficits induced by 56Fe radiation exposure.

Exposing rats to particles of high energy and charge (e.g., 56Fe) disrupts neuronal systems and the behaviors mediated by them; these adverse behavioral and neuronal effects are similar to those seen in aged animals. Because cognition declines with age, and our previous study showed that radiation disrupted Morris water maze spatial learning and memory performance, the present study used an 8-arm radial maze (RAM) to further test the cognitive behavioral consequences of radiation exposure. Control rats or rats exposed to whole-body irradiation with 1.0 Gy of 1 GeV/n high-energy 56Fe particles (delivered at the alternating gradient synchrotron at Brookhaven National Laboratory) were tested nine months following exposure. Radiation adversely affected RAM performance, and the changes seen parallel those of aging. Irradiated animals entered baited arms during the first 4 choices significantly less than did controls, produced their first error sooner, and also tended to make more errors as measured by re-entries into non-baited arms. These results show that irradiation with high-energy particles produces age-like decrements in cognitive behavior that may impair the ability of astronauts to perform critical tasks during long-term space travel beyond the magnetosphere.     

Battery charger design for the Columbus MTFF power system

A novel pulsewidth-modulated (PWM) dc-dc converter topology is proposed for the battery charge regulator (BCR) of the Columbus Man-Tended Free-Flyer (MTFF) power system. The system is a regulated bus system. Bus voltage control is implemented at the input of the BCR. The use of a conventional buck topology with PWM conductance control at the input results in a second-order behavior. A study of new PWM dc-dc converter topologies has been made to attain a suitable topology. The proposed converter topology is designed and a breadboard including the control loop has been built and tested. The experimental results show that the converter operates according to the design constraints.