Possible mechanisms of plant cell wall changes at microgravity.

Space and clinostatic experiments revealed that changes of plant cell wall structure and its function depend on type of tissue and duration of influence. It was shown that clinostat conditions reproduce the part of weightlessness biological effects. It is established that various responses of wall structural-metabolic organization occur at microgravity: changes of cell walls ultrastructure and organelles structure; decrease of synthesis of primary plant cell wall; rearrangements of polysaccharides content. It is shown that mechanisms of plant cell wall changes at microgravity are connected with decrease of cellulose crystallization, activation of pectolytic enzymes and rearrangement of calcium balance of apoplast and cytoplasm.     

Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

Chromosome instability of HPRT-mutant subclones induced by ionising radiation of various LET.

The induction of HPRT-mutations and survival of Chinese hamster cells (line B11ii-FAF28, clone 431) were studied after irradiation by 4He and 12C-ions of various LET (20-360 keV/micrometers), produced by the U-200 heavy ion accelerator. The RBE increases with LET up to the maximum at 100-200 keV/micrometers and then decreases. Cytogenetic analysis was performed on the HPRT-mutant subclones selected from unirradiated Chinese hamster V-79 cells and from HPRT-mutant subclones that arose after exposure to gamma-rays, 1 GeV protons and 14N-ions (LET-77 keV/micrometers), produced by the synchrophasotron and the U-400M heavy ion accelerator. Slow growing mutant subclones were observed. The cytogenetic properties of individual clones were highly heterogeneous and chromosome instability was observed in both spontaneous and radiation-induced mutants. Chromosome instability was highest among spontaneous mutants and decreased with increasing LET.     

Design of the TRIO system-on-chip for aerospace

Several design and testing aspects of the TRIO smart sensor data acquisition chip, developed by JHU/APL for NASA spacecraft applications are presented. TRIO includes a 10 bit self-corrected analog-to-digital converter (ADC), 16/32 analog inputs, a front end multiplexer with selectable aquisition time, a current source, memory, serial and parallel bus, and control logic. So far TRIO is used in many missions including Contour, Messenger, Stereo, Pluto, and the generic JPL X2000 spacecraft bus.     

Controlled solar sail transfers into the near-sun orbits

We present results of numerical simulations of trajectories of a space probe with a flat solar sail which moves from a circular Earth orbit to near-Sun regions. We consider the upper limit of the sail temperature as the basic restriction. We also examine the planar (ecliptic) solar sail transfer with gravity-assist flybys around planets.     

Perestroika and glasnost in the soviet space programme A personal view

For many years the world-famous Kettering Group of amateur satellite observers have confounded many more ‘official’ organization with their accucary in tracking Soviet space launches. In this article the group's founder, Geoffrey E. Perry, MBE, presents his personal perspective on current developments in the Soviet space programme. The USSR's increased openness about non-sensitive issues is to be welcomed, he concludes, but the Kettering Group has work to do yet.     

Next generation test support systems for the aircraft life cycle

The objective of this paper is to present the experience of EADS in using a common test support system for integration and qualification testing, aircraft ground equipment (AGE), in-service software maintenance facilities (ISMF) and ground crew training. EADS uses its test support system AIDASS (Advanced Integrated Data Acquisition and Stimulation System) in different phases of the aircraft life cycle. AIDASS is comprised of a real-time front-end system and a standard PC for the user interface. The EADS test support system is used in almost all phases of the aircraft life cycle, for instance, for integration and verification testing, as aircraft ground equipment, for in-service software maintenance and training of ground crews. This test support system is used for the software maintenance in the TORNADO program at different airforces in Europe. The airforces also share test setups with industry     

Analysis of queuing behavior of automatic ESM systems

Radar electronic support measures (ESM) systems detect active emitters in a given area and determine their identities and bearings. The high arrival rate of radar pulses in dense emitter environments demands fast automatic processing of arriving pulses so that the ESM system can fulfill its functions properly in real time. Yet, the performance analysis of automatic ESM system in real life Is difficult since both pulse arrivals and widths can be specified only probabilistically. The success of queuing theory in many applications such as computer communication networks and flow-control has encouraged designers to utilize queuing theory in qualifying and judging the performance of automatic ESM systems in dense emitter environments. The queuing behavior of these systems is analytically evaluated under different service disciplines and elaborate computer simulations validate the results. The analysis involves statistical modeling of arrival and departure processes as well as distribution of service times. It permits estimating the blocking probability due to high arrival rates of intercepted radar pulses or due to limited speed of the deinterleaver processor. Queuing analysis is shown to be quite useful to quantitatively assess tradeoffs in ESM systems design     

Time-dependent thermal X-ray afterglows from GRBS

Time-dependent thermal X-ray spectra are calculated from physically plausible conditions around GRB. It is shown that account for time-dependent ionization processes strongly affects the observed spectra of hot rarefied plasma. These calculations may provide an alternative explanation to the observed X-ray lines of early GRBs afterglows (such as GRB 011211). Our technique will allow one to obtain independent constraints on the GRB collimation angle and on the clumpiness of circumstellar matter.