Regulative development of Xenopus laevis in microgravity.

To test whether gravity is required for normal amphibian development, Xenopus laevis females were induced to ovulate aboard the orbiting Space Shuttle. Eggs were fertilized in vitro, and although early embryonic stages showed some abnormalities, the embryos were able to regulate and produce nearly normal larvae. These results demonstrate for the first time that a vertebrate can ovulate in the virtual absence of gravity, and that the eggs can develop to a free-living stage.     

Microorganisms and enzyme activity in permafrost after removal of long-term cold stress

Associations of immobilized microbial cells and organic-mineral complexes containing active enzymes are resistant to long-term (from tens of thousands to millions of years) effects of extremely low temperatures. This association enables the cells to restore their metabolic activity during permafrost thawing, because interactions with the heterogenous medium is made possible by the availability of active immobilized enzymes. The long-term effect of the cold probably favors an adaptational change of microbial metabolism that activates enzymes and cells during thawing.     

Delta-electron emission in fast heavy ion atom collisions.

Biological damages such as mutations, chromosomal aberrations etc. are a consequence of biochemical changes mostly in the DNA. With ionizing radiation, these chemical changes are due to primary ionization events and secondary ionization effects caused by the primarily produced electrons. Differences in the biological response of densely ionizing radiation, like heavy charged particles, in comparison to sparsely ionizing radiation, such as X- or gamma-rays, are mainly due to the differences in the production of the so called delta-electrons. Therefore, the emission process of electrons i.e. the cross section for the primary ionization event as well as the energy and angular distribution of the emitted electrons should be understood in detail. The delta-electron emission processes occuring in fast heavy ion atom collisions are explained qualitatively. The different spectral structures of electron emission arising from either the target or the projectile are explained in terms of simple models of the kinetics of momentum transfer induced by the COULOMB forces. In collisions of very heavy ions with matter, high nuclear COULOMB forces are created. These forces lead to a strong polarization of the electronic states of the participated electrons. The effects of this polarization are discussed.     

Fertilization and development of eggs of the South African clawed toad, Xenopus laevis, on sounding rockets in space.

Egg rotation and centrifugation experiments strongly suggest a role for gravity in the determination of the spatial structure of amphibian embryos. Decisive experiments can only be made in Space. Eggs of Xenopus laevis, the South African clawed toad, were the first vertebrate eggs which were successfully fertilized on Sounding Rockets in Space. Unfixed, newly fertilized eggs survived reentry, and a reasonable number showed a seemingly normal gastrulation but died between gastrulation and neurulation. Only a few reached the larval stage, but these developed abnormally. In the future, we intend to test whether this abnormal morphogenesis is due to reentry perturbations, or due to a real microgravity effect, through perturbation of the reinitiation of meiosis and other processes, or started by later sperm penetration.     

Identification and origin of plant pathogenic microorganisms in recirculating nutrient solutions.

Avoidance of root-infecting microorganisms was originally considered one of the advantages of cultivation of crops in a soilless, recirculating nutrient solution. However, to date, four viral, three bacterial and 21 fungal pathogens have been identified as causal agents of root disease in hydroponically-grown crops. Root-infecting fungi, particularly those which produce a motile stage known as a zoospore, have been the primary pathogens associated with extensive crop losses. Documented sources of these root pathogens in hydroponic systems include peat, surface water such as rivers and streams, and insects. The severity of disease caused by these introduced root pathogens is primarily governed by the genetic susceptibility of each crop and the temperature of the recirculating nutrient solution.     

Protein crystal growth aboard the U.S. space shuttle flights STS-31 and STS-32.

The first microgravity protein crystal growth experiments were performed on Spacelab I by Littke and John. These experiments indicated that the space grown crystals, which were obtained using a liquid-liquid diffusion system, were larger than crystals obtained by the same experimental system on earth. Subsequent experiments were performed by other investigators on a series of space shuttle missions from 1985 through 1990. The results from two of these shuttle flights (STS-26 and STS-29) have been described previously. The results from these missions indicated that the microgravity grown crystals for a number of different proteins were larger, displayed more uniform morphologies, and yielded diffraction data to significantly higher resolutions than the best crystals of these proteins grown on earth. This paper presents the results obtained from shuttle flight STS-32 (flown in January, 1990) and preliminary results from the most recent shuttle flight, STS-31 (flown in April, 1990).     

Precision orbit determination for TOPEX/Poseidon using TDRSS doppler tracking data

Precision orbit determination on the TOPEX/Poseidon (T/P) altimeter satellite is now being routinely achieved with sub-5cm radial and sub-15 cm total positioning accuracy using state-of-the-art modeling with precision tracking provided by a combination of: (a) global Satellite Laser Ranging (SLR) and Doppler Orbitography and Radiopositioning Integrated by Satellite (DORIS), or (b) the Global Positioning System (GPS) Constellation which provides pseudo-range and carrier phase observations. The geostationary Tracking and Data Relay Satellite System (TDRSS) satellites are providing the operational tracking and communication support for this mission. The TDRSS Doppler data are of high precision (0.3 mm/s nominal noise levels). Unlike other satellite missions supported operationally by TDRSS, T/P has high quality independent tracking which enables absolute orbit accuracy assessments. In addition, the T/P satellite provides extensive geometry for positioning a satellite at geostationary altitude, and thus the TDRSS-T/P data provides an excellent means for determining the TDRS orbits. Arc lengths of 7 and 10 days with varying degrees of T/P spacecraft attitude complexity are studied. Sub-meter T/P total positioning error is achieved when using the TDRSS range-rate data, with radial orbit errors of 10.6 cm and 15.5 cm RMS for the two arcs studied. Current limitations in the TDRSS precision orbit determination capability include mismodeling of numerous TDRSS satellite-specific dynamic and electronic effects, and in the inadequate treatment of the propagation delay and bending arising from the wet troposphere and ionosphere.     

Cloud profiling radar for the CloudSat mission

The Cloud Profiling Radar (CPR) for the upcoming CloudSat Mission is a spaceborne 94-GHz nadir-looking radar that measures the power backscattered by clouds as a function of distance from the radar. This sensor is expected to provide cloud measurements at a 500-m vertical resolution and a 1.5 km horizontal resolution. CPR will operate in a short-pulse mode and will yield measurements at a minimum detectable sensitivity of -28 dBZ.